COMPARATIVE ANALYSIS OF GUT BACTERIAL ABUNDANCE AND DIETARY HABITS OF TWO ETHNIC COMMUNITIES OF WEST BENGAL, INDIA - A PILOT STUDY

Souradip  Basu; Gaurab Aditya  Dhar; Kaustav Das; Mahashweta Mitra Ghosh; Rajat Banerjee; Subrata Sankar Bagchi; Sayak Ganguli

doi:10.48165/abr.2024.26.01.56

Authors

Souradip Basu Postgraduate and Research Department of Microbiology,Kolkata - 700 016, West Bengal (India)
Gaurab Aditya Dhar Postgraduate and Research Department of Biotechnology, St. Xavier's College (Autonomous), Kolkata - 700 016, West Bengal (India)
Kaustav Das Department of Anthropology, Bangabasi College, Kolkata - 700 009, West Bengal (India)
Mahashweta Mitra Ghosh Postgraduate and Research Department of Microbiology, Kolkata - 700 016, West Bengal (India)
Rajat Banerjee Department of Biotechnology and Dr. B.C. Guha Centre for Genetic Engineering and Biotechnology, University of Calcutta, Kolkata - 700 019, West Bengal (India)
Subrata Sankar Bagchi Dr. B.R. Ambedkar Chair, Department of Anthropology, University of Calcutta, Kolkata - 700 019, West Bengal (India)
Sayak Ganguli Postgraduate and Research Department of Biotechnology, St. Xavier's College (Autonomous), Kolkata - 700 016, West Bengal (India)

DOI:

https://doi.org/10.48165/abr.2024.26.01.56

Keywords:

Gut bacteriome, pristine gut, Sabar, Mech, metagenomics

Abstract

Tribal communities of West Bengal (India) are generously distributed in the major geographically distinct regions in easily distinguishable habitats. Most of these tribes prefer a non-urban lifestyle, both in the context of their livelihood and the maintenance of their diet.

Here, we report the gut bacterial assemblages along with the dietary habits of Proto-Australoid Sabar tribes of Purulia, Jhargram, and their adjacent villages, along with the foothill Mech tribes of Mongolian descent of North Kamakhyaguri in West Bengal.Anthropometric measures such as height, weight, and BMI were evaluated to understand the nutritional status of participants. In order to evaluate gut bacterial assembly, 16S rRNA from the first fecal matter was subjected to Illumina Miseq™ sequencing. Quality control (FASTQC), taxonomic (QIIME2), and functional (iVikodak) profiling were performed for metagenomic analysis.The study revealed that Holdemanella, Catenibacterium, and Bacteroides exhibited the highest abundances of 11.03%, 20.50%, and 35.91% across the Sabar male, female, and kid, respectively; while Prevotella, Bifidobacterium, and Bacteroides exhibited the highest abundances of 40.51%, 17.8%, and 29.96% across the Mech male, female, and kid, respectively.This study evaluated the interplay between dietary practices, indigenous lifestyle, gut bacteriome structure, and function in the Sabar and Mech tribes.

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