ASSESSMENT OF Mycobacterium paratuberculosis BIO-LOAD IN PATIENTS SUFFERING WITH THYROID AND ARTHRITIS DISORDERS USING MULTIPLE TESTS
DOI:
https://doi.org/10.48165/abr.2024.26.01.63Keywords:
Crohn’s disease, Indian bison type, IS900 PCR, indigenous ELISA, Mycobacterium avium subspecies paratuberculosisAbstract
Johne's disease in ruminants is caused by Mycobacterium avium paratuberculosis (MAP), which has been linked to inflammatory bowel disease and Crohn's disease in humans. Milk from infected ruminants is considered the primary source of transmission. This study aimed to estimate the presence of MAP in clinical samples (blood and serum) from confirmed thyroid and arthritis cases. Samples were collected from the Community Healthcare Center, Bhangel campus, Noida NCR region, India.A total of 111 blood samples (76 thyroid and 35 arthritis) were collected and analyzed using IS900 blood PCR, indigenous ELISA, and TaqMan probe qPCR IS1311 PCR_REA. Among the serum samples, 19 (17.1%) tested positive for MAP antibodies via ELISA (thyroid: 18.4%; arthritis: 14.2%). For the blood samples, MAP was detected in 10.8% and 11.7% of cases using IS900 PCR and TaqMan probe qPCR, respectively.The bio-incidence of MAP in thyroid patients ranged from 9.2–14.2%, while in arthritis patients it ranged from 10.5–14.2%, as determined by IS900 PCR and TaqMan probe qPCR. A substantial agreement was observed between ELISA and PCR results. Molecular strain typing using IS1311 PCR_REA identified the prevalent biotype as the ‘Indian Bison type.’This study suggests moderate exposure of the human population to MAP in the Noida NCR region. The presence of the ‘Indian Bison type’ biotype indicates a potential zoonotic transmission pathway from cattle products to humans. To validate these findings, further research with larger cohorts of thyroid and arthritis patients is recommended.
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Aitken, J.M., Aitken, J.E. and Agrawal, G. 2024. Mycobacterium avium ssp. paratuberculosis and Crohn’s disease-diagnostic microbiological investigations can inform new therapeutic approaches. Antibiotics, 13(2): 158. [https://doi.org/10.3390/antibiotics13020158].
Azzam, N., Nounou, H., Alharbi, O., Aljebreen, A. and Shalaby, M. 2012. CARD15/NOD2, CD14 and Toll-like 4 receptor gene polymorphisms in Saudi patients with Crohn’s disease. International Journal of Molecular Sciences, 13(4): 4268. [https://doi.org/10.3390/ijms13044268].
Chaubey, K.K., Gupta, R.D., Gupta, S., Singh, S.V., Bhatia, A.K., Jayaraman, S., et al., 2016. Trends and advances in the diagnosis and control of paratuberculosis in domestic livestock. Veterinary Quarterly, 36(4): 203-227.
Chaubey, K.K., Singh, S.V., Singh, P.K., Gupta, S., Khandelwal, V., Choudhary, P.K., et al., 2020. Detection of anti-Mycobacterium avium subspecies paratuberculosis antibodies in thyroid and type-1 diabetes patients. Indian Journal of Biotechnology, 19: 49-52.
Collins, M.T. 2002. Interpretation of a Commercial Bovine Paratuberculosis Enzyme-Linked Immunosorbent Assay by Using Likelihood Ratios. Clinical and Diagnostic Laboratory Immunology, 9(6): 1367-1371.
Collins, M.T., Lisby, G., Moser, C., Chicks, D., Christensen, S., Reichelderfer, M., et al., 2000. Results of multiple diagnostic tests for Mycobacterium avium subsp. paratuberculosis in patients with inflammatory bowel disease and in controls. Journal of Clinical Microbiology, 38(12): 4373-4381.
Cossu, A., Ferrannini, E., Fallahi, P., Antonelli, A. and Sechi, L.A. 2013. Antibodies recognizing specific Mycobacterium avium subsp. paratuberculosis MAP3738c protein in type 1 diabetes mellitus children are associated with serum Th1 (CXCL10) chemokine. Cytokine, 61: 337-339.
D’Amore, M., Lisi, S., Sisto, M., Cucci, L. and Dow, C.T. 2010. Molecular identification of Mycobacterium avium subspecies paratuberculosis in an Italian patient with Hashimoto’s thyroiditis and Melkersson-Rosenthal syndrome. Journal of Medical Microbiology, 59: 137-139.
Dow, C.T. 2011. Mycobacterium paratuberculosis and autism: is this a trigger? Medical Hypotheses, 77(6): 977-981.
558 Sheetal Rajput and Saurabh Gupta
Dow, C.T. 2024. Mycobacterium avium ss. paratuberculosis and human disease: Bridging infection and autoimmunity. Infection and Autoimmunity, 2024. 559-581. [https://doi.org/10.1016/B978- 0-323-99130-8.00026-X].
Dow, C.T. and Alvarez, B.L. 2022. Mycobacterium paratuberculosis zoonosis is a one health emergency. Ecohealth, 19(2), 164-174.
Fiocchi, C. 1998. Inflammatory bowel disease: etiology and pathogenesis. Gastroenterology, 115(1): 182-205.
Goyette, P., Labbe, C., Trinh, T.T., Xavier, R.J. and Rioux, J.D. 2007. Molecular pathogenesis of inflammatory bowel disease: Genotypes, phenotypes and personalized medicine. Annals of Medicine, 39(3): 177-199.
Greenstein, R.J. 2003. Is Crohn’s disease caused by a mycobacterium? Comparisons with leprosy, tuberculosis, and Johne’s disease. Lancet Infectious Diseases, 3(8): 507-514. Gupta, S., Chaubey, K.K., Agarwal, P., Kuenstner, J.T., Parashar, D. and Singh, S.V. 2021. Therapeutic management of Mycobacterium avium subspecies paratuberculosis infection with complete resolution of symptoms and disease in a patient with advanced inflammatory bowel syndrome. Molecular Biology Reports, 48(10): 7013-7020.
Gupta, S., Singh, S.V., Gururaj, K., Chaubey, K.K., Singh, M., Lahiri, B., et al., 2017. Comparison of IS900 PCR with ‘Taqman probe PCR’and ‘SYBR green Real time PCR’assays in patients suffering with thyroid disorder and sero-positive for Mycobacterium avium subspecies paratuberculosis. Indian Journal of Biotechnology, 16(2): 228-234.
Gupta, S., Singh, S.V., Singh, M., Chaubey, K.K., Karthik, K., Bhatia, A.K., et al., 2019. Vaccine approaches for the “therapeutic management” of Mycobacterium avium subspecies paratuberculosis infection in domestic livestock. The Veterinary Quarterly, 39(1): 143-152.
Hemati, Z., Derakhshandeh, A., Haghkhah, M., Chaubey, K. K., Gupta, S., Singh, M., et al., 2019. Mammalian cell entry operons; novel and major subset candidates for diagnostics with special reference to Mycobacterium avium subspecies paratuberculosis infection. Veterinary Quarterly, 39(1): 65-75.
Jones, D.B., Coulson, A.F.W. and Duff, G.W. 1993. Sequence homologies between hsp60 and autoantigens. Immunology Today, 14(3): 115-118.
Kayali, S., Fantasia, S., Gaiani, F., Cavallaro, L.G., Luigi De’angelis, G., Laghi, L. and Gavazzeni, H. 2024. NOD2 and Crohn’s disease clinical practice: From epidemiology to diagnosis and therapy, Rewired. Inflammatory Bowel Diseases. izae075. [https://doi.org/10.1093/ibd/izae075].
Singh, S.V., Sohal, J.S., Kumar, N., Gupta, S., Chaubey, K.K., Rawat, K.D., et al., 2014. Recent approaches in diagnosis and control of mycobacterial infections with special reference to Mycobacterium avium subspecies paratuberculosis. Advances in Animal Veterinary Sciences, 2 (1S): 1-12.
Kumar, S., Singh, S.V., Singh, A.V., Singh, P.K., Sohal, J.S. and Maitra, A. 2010. Wildlife (Boselaphus tragocamelus)–small ruminant (goat and sheep) interface in the transmission of ‘Bison type’ genotype of Mycobacterium avium subspecies paratuberculosis in India. Comparative Immunology, Microbiology and Infectious Diseases, 33(2): 145-159.
Millar, D., Ford, J., Sanderson, J., Withey, S., Tizard, M., Doran, T. and Hermon-Taylor, J. 1996. IS900 PCR to detect Mycobacterium paratuberculosis in retail supplies of whole pasteurized cows’ milk in England and Wales. Applied and Environmental Microbiology, 62(9): 3446-3452.
Moghadam, M., Ghaemi, E.A., Akbari, H., Razavi Nikoo, H., and Zamani, S. 2022. Mycobacterium avium subsp. paratuberculosis and Hashimoto’s thyroiditis: Is MAP the trigger? Frontiers in Cellular and Infection Microbiology, 12: 972929. [doi: 10.3389/fcimb.2022.972929].
Momotani, E., Romona, N.M., Yoshihara, K., Momotani, Y., Hori, M., Ozaki, H., et al., 2012. Molecular pathogenesis of bovine paratuberculosis and human inflammatory bowel diseases. Veterinary Immunology and Immunopathology, 148(1–2): 55-68.
Moudgil, K.D., Chang, T.T., Eradat, H., Chen, A.M., Gupta, R.S., Brahn, E. and Sercarz, E.E. 1997. Diversification of T cell responses to carboxy-terminal determinants within the 65-kD heat-
Mycobacterium paratuberculosis bio-load assessment in patients 559
shock protein is involved in regulation of autoimmune arthritis. The Journal of Experimental Medicine, 185(7): 1307-1316.
Naser, S.A., Collins, M.T., Crawford, J.T., and Valentine, J.F. 2009. Culture of Mycobacterium avium subspecies paratuberculosis (MAP) from the blood of patients with Crohn’s disease: A follow up blind multi center investigation. The Open Inflammation Journal, 2: 22-23.
Naser, S.A., Ghobrial, G., Romero, C. and Valentine, J.F. 2004. Culture of Mycobacterium avium subspecies paratuberculosis from the blood of patients with Crohn’s disease. Lancet, 364(9439): 1039–1044.
Naser, S.A., Thanigachalam, S., Dow, C.T. and Collins, M.T. 2013. Exploring the role of Mycobacterium avium subspecies paratuberculosis in the pathogenesis of type 1 diabetes mellitus: A pilot study. Gut Pathogens, 5(1): 14. [https://doi.org/10.1186/1757-4749-5-14].
Ogura, Y., Bonen, D.K., Inohara, N., Nicolae, D.L., Chen, F.F., Ramos, R. et al., 2001. A frameshift mutation in NOD2 associated with susceptibility to Crohn’s disease. Nature, 411(6837): 603- 606.
Ozana, V., Hruska, K. and Sechi, L.A. 2022. Neglected facts on Mycobacterium avium subspecies paratuberculosis and type 1 diabetes. International Journal of Molecular Sciences, 23(7): 3657- [doi: 10.3390/ijms23073657}.
Sechi, L.A., Rosu, V., Pacifico, A., Fadda, G., Ahmed, N. and Zanetti, S. 2008. Humoral immune responses of type 1 diabetes patients to Mycobacterium avium subsp. paratuberculosis lend support to the infectious trigger hypothesis. Clinical and Vaccine Immunology, 15(2): 320-326.
Sevilla, I., Singh, S.V, Garrido, J.M., Aduriz, G., Rodríguez, S., Geijo, M.V. et al., 2005. Molecular typing of Mycobacterium avium subspecies paratuberculosis strains from different hosts and regions. Revue Scientifique et Technique, 24(3): 1061-1066.
Shankar, H., Singh, S.V., Singh, P.K., Singh, A.V., Sohal, J.S. and Greenstein, R.J. 2010. Presence, characterization, and genotype profiles of Mycobacterium avium subspecies paratuberculosis from unpasteurized individual and pooled milk, commercial pasteurized milk, and milk products in India by culture, PCR, and PCR-REA methods. International Journal of Infectious Diseases,
14(2): 121-126.
Singh, A.V., Singh, S.V., Singh, P.K., Sohal, J.S. and Singh, M.K. 2011. High prevalence of Mycobacterium avium subspecies paratuberculosis (‘Indian bison type’) in animal attendants suffering from gastrointestinal complaints who work with goat herds endemic for Johne’s disease in India. International Journal of Infectious Diseases, 15(10): e677-e683.
Singh, S.V., Kuenstner, J.T., Davis, W.C., Agarwal, P., Kumar, N., Singh, D., Gupta, S. et al., 2016. Concurrent resolution of chronic diarrhea likely due to Crohn’s disease and infection with Mycobacterium avium paratuberculosis. Frontiers in Medicine, 3: 49. [doi: 10.3389/fmed.2016.00049].
Singh, S.V., Singh, P.K., Singh, A.V., Sohal, J.S., Kumar, N., Chaubey, K.K. et al., 2014a. ‘Bio-load’ and bio-type profiles of Mycobacterium avium subspecies paratuberculosis infection in the domestic livestock population endemic for Johne’s disease: A survey of 28 years (1985-2013) in India. Transboundary and Emerging Diseases, 61: 43-55.
Singh, S.V., Singh, A.V., Singh, P.K., Gupta, V.K., Kumar, S. and Vohra, J. 2007. Sero-prevalence of paratuberculosis in young kids using ‘Bison type’, Mycobacterium avium subsp. paratuberculosis antigen in plate ELISA. Small Ruminant Research, 70(2-3): 89-92.
Singh, S.V., Kumar, N., Sohal, J.S., Singh, A.V., Singh, P.K., Agrawal, N.D. et al., 2014b. First mass screening of the human population to estimate the bio-load of Mycobacterium avium sub-species paratuberculosis in North India. Journal of Public Health and Epidemiology, 6(1): 20-29.
Whittington, R.J., Marsh, I.B. and Whitlock, R.H. 2001. Typing of IS 1311 polymorphisms confirms that bison (Bison bison) with paratuberculosis in Montana are infected with a strain of Mycobacterium avium subsp. paratuberculosis distinct from that occurring in cattle and other domesticated livestock. Molecular and Cellular Probes, 15(3): 139-145.
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