ASYMBIOTIC SEED GERMINATION OF AN ANTICANCEROUS TERRESTRIAL ORCHID Eulophia nuda Lindl. AND ITS ADULTERANT Geodorum densiflorum (Lam) Schltr.
DOI:
https://doi.org/10.48165/abr.2024.26.01.30Keywords:
Adulteration, anticancer properties, asymbiotic seed germination, Eulophia, Geodorum, Ex situ conservationAbstract
Eulophia nuda Lindl. corms are used to cure cancer and is overexploited by the traditional medicine practitioners and medicinal plant traders. The corms of Geodorum densiflorum look morphologically similar to the corms of Eulophia nuda, hence it is used as an adulterant to the corms of Eulophia nuda, consequetly, the population of G. densiflorum is also facing the exploitation pressure. In present study, asymbiotic seed germination of both the terrestrial orchids was achieved using six media like Vacin and Went (WV) medium, Malmgren modified (MM) terrestrial orchid medium, modified Kundson C medium, ½ MS medium, Lindemann medium (LM) and BM1 medium along with three photoperiods (0/24, 16/8, and 24/0 h L/D). Eulophia nuda showed highest asymbiotic seed germination on MM medium (80.55 %) and the best light treatment was 0/24 h L/D. The highest asymbiotic seed germination of G densiflorum was on VW (84.07%), LM (83.29%) and BM 1(81.02%) and the best light treatment was 24/0 h L/D.
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Arditti, J., Michaud, J.D. and Oliva, A.P. 1981. Seed germination of North American orchids. I. Native California and related species of Calypso, Epipactis, Goodyera, Piperia, and Platanthera. Botanical Gazette, 142(4): 442-453.
Bhadra, S. and Hossain, M. 2003. In vitro germination and micropropagation of Geodorum densiflorum (Lam.) Schltr., an endangered orchid species. Plant Tissue Culture, 13: 165-171. Bhatt, D.R., Jethva, K.D. and Zaveri, M.N. 2018. In-vitro cytotoxicity studies of the therapeutic orchid: Eulophia nuda. Journal of Pharmacognosy and Phytochemistry, 7: 680–683. Chang, C., Hu, W.H., Chen, Y.C., Su, Y.L and Chiu, Y.T. 2010. In vitro flowering and mating system of Eulophia graminea Lindl. Botanical Studies, 51: 357-362.
Dawande, V. and Gurav, R. 2015. In-vitro seed germination and effect of growth regulators on subsequent development of protocorms of Eulophia nuda Lindl. International Journal of Applied Sciences and Biotechnology, 3: 243-247.
Uma Thakur and Nitin Dongarwar
Dongarwar, N. and Thakur, U. 2014. Artificial pollination and its role in orchid conservation. A glimpse of current vistas in Plant Science Research. Nagpur, India. Hislop College Publication Cell. 10: 123-140.
Galetto, L., Bernardello, G. and Rivera, G.L. 1997. Nectar, nectaries, flower visitors, and breeding system in five terrestrials Orchidaceae from central Argentina. Journal of Plant Research, 110: 393-403.
Gunawan, E., Agustini, V. and Simaremare, E.S. 2020. Investigation phytochemical screening, antibacterial activity, and cytotoxic ethanolic extract of Geodorum densiflorum (lam.) Schltr from Papua. International Journal of Pharmaceutical Research, 12: 323-329
Hada, S., Yadav, D.K., Roat, P. and Kumari, N. 2020. Eulophia nuda: A review of its traditional uses, phytochemistry and pharmacology. Pharmaceutical Chemistry Journal, 54: 40–45. Hossain, M.S., Sayeed, Mohammed Abu, Sayeed, Mohammed Aktar, Ehsanul, M. and Chowdhury, H. 2012. Investigation on in-vitro cytotoxic, antibacterial and phytochemical screening of ethyl acetate extract of Geodorum densiflorum (Lam.) Schltr. The Pharma Innovation 1: 108-113. Jagtap, S., Deokule, S. and Bhosle, S. 2008. Ethnobotanical uses of endemic and RET plants by Pawra tribe of Nandurbar district, Maharashtra. Indian Journal of Traditional Knowledge, 7(2): 311- 315
Johnson, T.R., Stewart, S.L., Dutra, D., Kane, M.E. and Richardson, L. 2007. Asymbiotic and symbiotic seed germination of Eulophia alta (Orchidaceae) - Preliminary evidence for the symbiotic culture advantage. Plant Cell Tissue Organ Culture, 90: 313-323.
Kabir, K.M.A., Amin, R., Hasan, I., Asaduzzaman, A.K.M., Khatun, H. and Kabir, S.R. 2020. Corrigendum to Geodorum densiflorum rhizome lectin inhibits Ehrlich ascites carcinoma cell growth by inducing apoptosis through the regulation of BAX, p53 and NF-κB genes expression. International Journal of Biological Macromolecules. 125: 92-98.
Keerthiga, M. and Anand, S.P. 2014. Physicochemical, preliminary phytochemical analysis and antibacterial activity against clinical pathogens of medicinally important orchid Geodorum densiflorum (Lam) Schltr. International Journal of Pharmacy and Pharmaceutical Sciences, 6: 558-561.
Khatun, F., Nasrin, N., Monira, S., Asaduzzaman, M. and Apu, A.S. 2013. Assessment of neuropharmacological and analgesic potentials of Geodorum densiflorum (Lam.) Schltr. root extracts in experimental animals. Pharmacology Online, 3: 16-22.
Kshirsagar, R.D., Kanekar, Y.B., Jagtap, S.D., Upadhyay, S.N., Rao, R., Bhujbal, S.P. and Kedia, J.N. 2010. Phenanthrenes of Eulophia ochreata Lindl. International Journal of Green Pharmacy. 4: 147-152.
McAlister, B.G. and Van Staden, J. 1998. In vitro culture of Eulophia species. South African Journal of Botany, 64: 264-266.
Muthukrishnan, S., Kumar, T.S. and Rao, M.V. 2013. Effects of different media and organic additives on seed germination of Geodorum densiflorum (Lam) Schltr.—An endangered orchid. International Journal of Scientific Research 2: 23-26.
Nagulwar, V. and Nandgave, M. 2016. Pharmacognostic and pharmacological activity of Eulophia nuda Lind - A review. International Journal of Pharma and Bio Sciences, 7(3): 308-311. Narkhede, A., Kasote, D., Kuvalekar, A., Harsulkar, A. and Jagtap, S. 2016. Amarkand: A comprehensive review on its ethnopharmacology, nutritional aspects and taxonomy. Journal of Intercultural Ethnopharmacology, 5(2): 198-204.
Rasmussen, H., Andersen, T.F. and Johansen, B. 1990. Temperature sensitivity of in vitro germination and seedling development of Dactylorhiza majalis (Orchidaceae) with and without a mycorrhizal fungus. Plant, Cell and Environment, 13(2): 171-177.
Roy, J. and Banerjee, N. 2001. Cultural requirements for in vitro seed germination, protocorm growth and seedling development of Geodorum densiflorum (Lam.) Schltr. Indian Journal of Experimental Biology, 39: 1041-1047.
Shroti, M.R.K. and Upadhyay, R. 2014. In vitro micropropagation of Eulophia nuda Lind an endangered terrestrial orchid through plb’ (protocorm like bodies). International Journal of Plant, Animal and Environmental Sciences, 4(1): 172-176.
Singh, A. and Duggal, S. 2009. Medicinal orchids - An overview. Ethnobotanical Leaflets 3: 3-9 Thakur, U. and Dongarwar, N. 2017. Asymbiotic seed germination, seedling development and tuberization of Peristylus lawii Wight (Orchidaceae), a rare terrestrial orchid. Hislopia Journal. 10: 59-72.
Thomas, P., Newman, M., Svengsuksa, B. and Ketphanh, S. 2006. A Review of CITES Appendices I and II Plant Species. From Lao PDR. 3-52. Darwin Initiative for the Survival of the Species Project 163-13-007. Khammouane Province, Lao PDR, Vietnam.
Tuchinda, P., Udchachon, J., Khumtaveeporn, K., Taylor, W.C., Engelhardt, L.M. and White, A.H. 1988. Phenanthrenes of Eulophia nuda. Phytochemistry, 27: 3267-3271.
Van Waes, J.M. and Debergh, P.C. 1986. In vitro germination of some Western European orchids. Physiologia Plantarum, 67(2): 253-261.
Yamazaki, J. and Miyoshi, K. 2006. In vitro asymbiotic germination of immature seed and formation of protocorm by Cephalanthera falcata (Orchidaceae). Annals of Botany, 98(6): 1197-1206. Zettler, LW. and McInnis Jr., T.M. 1994. Light enhancement of symbiotic seed germination and
development of an endangered terrestrial orchid (Platanthera integrilabia). Plant Science, 102: 133-138.
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