DETERMINATION OF ANTIFUNGAL AND ANTIOXIDANT POTENTIAL OF LEAF AND FRUIT EXTRACTS OF GUAVA (Psidium guajava Linn.)
DOI:
https://doi.org/10.48165/abr.2024.26.01.26Keywords:
Antifungal, antioxidant, CAT, DPPH, extracts, FRAP, PODAbstract
The present study was aimed to evaluate the antifungal and antioxidant properties of leaf and fruit extracts of guava (Psidium guajava Linn.). Antifungal assays were conducted to assess the efficacy of extracts against clinically relevant fungal strains, viz., Aspergillus niger MTCC-4325, Penicillium chrysogenum MTCC-2539, Candida albicans MTCC-7315, and Fusarium solani MTCC-3309. Besides, enzyme activity for catalase (CAT) and peroxidase (POD) were assayed to elucidate the antioxidant potential of extracts. The radical scavenging activities of extracts were determined using 2,2-diphenyl-1-picrylhydrazyl (DPPH), and ferric reducing antioxidant power (FRAP) assays to quantify the overall antioxidant capacity. Results indicated pronounced inhibitory activity against F. solani in methanolic extracts of leaves (21.66 ± 2.05) and fruits (24.66 ± 1.25), with aqueous extracts exhibiting comparatively lesser inhibition across the tested fungal strains. Moreover, the enzyme activity assays revealed higher CAT and POD activities in fresh leaf extract as compared to the fruit extract. Notably, DPPH assay underscored the superior antioxidant potential of Psidium leaf extracts relative to fruit extracts, while FRAP assay showed higher radical scavenging capacity of methanolic extract as compared to the aqueous extract.
Downloads
References
Afanyibo, Y.G. 2018. Antimicrobial activities of Syzygium aromaticum (L.) Merr. & LM Perry (myrtaceae) fruit extracts on six standard microorganisms and their clinical counterpart. Open Access Library Journal, 5(12): 1. [https://doi.org/10.4236/oalib.1104951].
Alisi, C.S., Asiwe, E.S., Ene, C.A. and Alisi, P.N. 2018. Antioxidant and free radical scavenging properties of aqueous extract of Psidium guajava leaf. FUTO Journal Services, 4(1): 222-234. Batiha, G.E.S., Alkazmi, L.M., Wasef, L.G., Beshbishy, A.M., Nadwa, E.H. and Rashwan, E.K. 2020. Syzygium aromaticum L. (Myrtaceae): Traditional uses, bioactive chemical constituents, pharmacological and toxicological activities. Biomolecules. 10(2): 202. [https://doi.org/ 10.3390%2Fbiom10020202].
Antifungal and antioxidant potential of guava extract 231
Benzie, I.F. and Strain, J.J. 1996. The ferric reducing ability of plasma (FRAP) as a measure of antioxidant power: The FRAP assay. Analytical Biochemistry, 239(1): 70-76. Berkow, E.L., Lockhart, S.R. and Ostrosky-Zeichner, L. 2020. Antifungal susceptibility testing: Current approaches. Clinical Microbiology Reviews, 33(3): 10-1128. [https://doi.org/10.1128/ cmr.00069-19].
Besufekad, S.Y., Mekdes, M., Abebech, M., Delesa, D., Tekalign, D., Demitu, K. and Birtukan, B. 2017. The antimicrobial activity of leaf extracts of Myrtus communis. Journal of Microbial and Biochemical Technology, 9(6): 290-292.
Beyhan, Ö., Elmastas, M. and Gedikli, F. 2014. Total phenolic compounds and antioxidant capacity of leaf, dry fruit and fresh fruit of feijoa (Acca sellowiana, Myrtaceae). Journal of Medicinal Plants Research, 4(11): 1065-1072.
Bogha, T.T., Sawate, A.R., Kshirsagar, R.B. and Bochare, S.S. 2020. Studies on physical, chemical and mineral evaluation of guava (Psidium guajava L.). Pharma Innovation Journal, 9(3): 117- 119.
Burcher, J.T., DeLiberto, L.K., Allen, A.M., Kilpatrick, K.L. and Bishayee, A. 2023. Bioactive phyto compounds for oral cancer prevention and treatment: A comprehensive and critical evaluation. Medicinal Research Reviews. [https://doi.org/10.1002/med.21969].
Chandra, A.S. and Radhamany, P.M. 2022. Evaluation of in vitro antioxidant potential in different parts of Syzygium caryophyllatum (L.) Alston. Medicinal Plants-International Journal of Phytomedicines and Related Industries, 14(1): 153-161.
Das, M. and Goswami, S. 2019. Antifungal and antibacterial property of guava (Psidium guajava) leaf extract: Role of phytochemicals. International Journal of Health Science and Research, 9(2): 39-45.
de Araujo, F.F., Neri-Numa, I.A., de Paulo, Farias, D., da Cunha, G.R.M.C. and Pastore, G.M. 2019. Wild Brazilian species of Eugenia genera (Myrtaceae) as an innovation hotspot for food and pharmacological purposes. Food Research International, 121: 57-72.
Fierro, F., Vaca, I., Castillo, N.I., García-Rico, R.O. and Chávez, R. 2022. Penicillium chrysogenum, A vintage model with a cutting-edge profile in biotechnology. Microorganisms, 10(3): 573. [https://doi.org/10.3390/microorganisms10030573].
Gulcin, İ. 2020. Antioxidants and antioxidant methods: An updated overview. Archives of Toxicology, 94(3): 651-715.
Haida, Z. and Hakiman, M. 2019. A comprehensive review on the determination of enzymatic assay and nonenzymatic antioxidant activities. Food Science & Nutrition, 7(5): 1555-1563. Kaiser, A.E., Baniasadi, M., Giansiracusa, D., Giansiracusa, M., Garcia, M., Fryda, Z. and Bishayee, A. 2021. Sulforaphane: A broccoli bioactive phytocompound with cancer preventive potential. Cancers, 13(19): 4796. [https://doi.org/10.3390%2Fcancers13194796]. Khalaf, O.M., Abdel-Aziz, M.S., El-Hagrassi, A.M., Osman, A.F. and Ghareeb, M.A. 2021. Biochemical aspect, antimicrobial and antioxidant activities of Melaleuca and Syzygium species (Myrtaceae) grown in Egypt. International Journal of Physics: Conference Series, 1879: 2, p. 022062. [https://doi.org/10.1088/1742-6596/1879/2/022062].
Kiran, M.S., Betageri, V.S., Kumar, C.R., Vinay, S.P. and Latha, M.S. 2020. In-vitro antibacterial, antioxidant and cytotoxic potential of silver nanoparticles synthesized using novel Eucalyptus tereticornis leaves extract. Journal of Inorganic and Organometallic Polymers and Materials, 30: 2916-2925.
Mohammed, A.E. 2015. Green synthesis, antimicrobial and cytotoxic effects of silver nanoparticles mediated by Eucalyptus camaldulensis leaf extract. Asian Pacific Journal of Tropical Biomedicine, 5(5): 382-386.
Naglik, J.R., Gaffen, S.L. and Hube, B. 2019. Candidalysin: Discovery and function in Candida albicans infections. Current Opinion in Microbiology, 52: 100-109.
Nucci, M. and Anaissie, E. 2023. Invasive fusariosis. Clinical Microbiology Reviews, e00159-22. [https://doi.org/10.1128%2Fcmr.00159-22].
Richa Bhardwaj et al.
Oluwajobi, I., Kabiru, Y.A. and Jigam, A.A. 2019. Antibacterial and antifungal activities of aqueous leaves extract of some medicinal plants. GSC Biological and Pharmaceutical Sciences, 9(1): 62- 69.
Qamar, M., Akhtar, S., Ismail, T., Yuan, Y., Ahmad, N., Tawab, A., Ismail, A., Barnard, R.T., Cooper, M.A., Blaskovich, M.A.T. and Ziora, Z.M. 2021. Syzygium cumini (L.) Skeels fruit extracts: In vitro and in vivo anti-inflammatory properties. Journal of Ethnopharmacology, 271: 113805. [https://doi.org/10.1016/j.jep.2021.113805].
Sen, A. and Batra, A. 2012. Evaluation of antimicrobial activity of different solvent extracts of medicinal plant: Melia azedarach L. International Journal of Current Pharmaceutical Research, 4(2): 67-73.
Sita, K., Sehgal, A., Kumar, S. and Nayyar, H. 2022. Individual and combined effects of selenium and silica on enhancing the heat tolerance of lentil (Lens culinaris Medik.) genotypes. Plant Physiology Reports, 27(3): 481-497.
Talapko, J., Juzbašić, M., Matijević, T., Pustijanac, E., Bekić, S., Kotris, I. and Škrlec, I. 2021. Candida albicans - The virulence factors and clinical manifestations of infection. Journal of Fungi, 7(2): 79. [https://doi.org/10.3390/jof7020079].
Yu, J., Yang, M., Han, J. and Pang, X. 2022. Fungal and mycotoxin occurrence, affecting factors, and prevention in herbal medicines: A review. Toxin Reviews, 41(3): 976-994.
Zahin, M., Ahmad, I. and Aqil, F. 2017. Antioxidant and antimutagenic potential of Psidium guajava leaf extracts. Drug and Chemical Toxicology, 40(2): 146-153.
Downloads
Published
Issue
Section
How to Cite
