LARVICIDAL ACTIVITY OF Parthenium hysterophorus EXTRACTS, PREPARED IN HEXANE, ACETONE AND METHANOL SOLVENTS AGAINST Aedes aegypti MOSQUITO
DOI:
https://doi.org/10.48165/abr.2024.26.01.54Keywords:
Aedes aegypti, Botanicals, larvicidal, Parthenium hysterophorusAbstract
Aedes aegypti (A. aegypti) is responsible for higher rate of dengue, yellow fever and chikungunya in tropical and subtropical areas including India. We have examined the presence of bioactive compounds and larvicidal capability of Parthenium hysterophorus (P. hysterophorus) arial part extracts in the solvents of acetone, methanol and hexane against A. aegypti. Fully fed larva were collected from different residential areas of Gorakhpur and identified as A. aegypti larvae. Four aliquots 250, 500, 750 and 1000, mg/L used for 50% to 95% mortality in 24, 48, 72 and 96 hours (h). Phytochemical analysis revealed that methanol extract had maximum rich in saponin, flavonoid, terpenoid, alkaloid and phenols followed by acetone. But, hexane extracts showed only terpenoid and alkaloid activity. The hexane solvent extract showed the LC50 and LC90 were 1920.05, 1496.72, 979.83, 337.84 and 18883.78, 14213.81, 16754.35 and 1246.1 4, mg/L in 24 to 96 h, respectively. Acetone extracts also showed similar values. However, the methanolic extracts had the LC50 and LC90 as 1351.14, 1029,62, 657.77, 226.29 and 5618.12, 5995.96, 2860.23, 804.75. mg/L from 24-96 h. Our study showed that arial part of P. hysterophorus exhibited maximum toxicity in methanol solvent followed by acetone and hexane against A. aegypti larvae.
Downloads
References
Abbott, W.S. 1925. A method of computing the effectiveness of insecticides. Journal of Economic Entomology, 18: 265-267.
Amir, H., Butt, B.Z. and Vehra, S.E. 2017. Evaluation of larvicidal activity of Parthenium hysterophorus against Aedes aegypti. International Journal of Mosquito Research, 4: 1-4. Bansode, P.A., Ingole, K.B., Godase, P.N., Pawar, S.G. and Bhosale, V.S. 2016. Evaluation of mosquito larvicidal activity of Parthenium hysterophorus (Congress weed) against Culex quinquefasciatus. British Journal of Pharmaceutical and Medical Research, 1: 29-33.
472 Sanjeev Kumar and Sushil Kumar
Bilal, H. and Hassan, S.A. 2012. Plants secondary metabolites for mosquito control. Asian Pacific Journal of Tropical Disease, 2: 168-168.
Colon-Gonzalez, F.J., Sewe, M.O., Tompkins, A.M., Sjodin, H. and Casallas, A. 2021. Projecting the risk of mosquito-borne diseases in a warmer and more populated world: A multi-model, multi scenario intercomparison modelling study. Lancet Planetary Health, 5: 404-414.
Darsie, R.F. and Samanidou-Voyadjoglou, A.N. 1997. Keys for the identification of the mosquitoes of Greece. Journal of the American Mosquito Control Association-Mosquito News, 13: 247-254. Evans, H.C. 1997. Parthenium hysterophorus: A review of its weed status and the possibilities for biological control. Biocontrol News and Information, 18: 89-98.
Ghimire, K., Banerjee, J., Gupta, A.K. and Dahal, P. 2015. Phytochemical constituents and pharmacological uses of medicinal plant Achyranthes aspera: A review. World Journal of Pharmaceutical Research, 4: 470-489.
Goyal, M.H. and Shinde, L.V. 2020. Mosquito larvicidal efficacy of methanolic extract from seeds of Datura inoxia Mill. against Aedes aegypti (Linn.) with insight into GC-MS analysis. Journal of Entomological Research, 44: 107-112.
Harborne J.B. 1984. Phytochemical Methods: A Guide to Modern Techniques of Plant Analysis. Chapman and Hall. London, UK.
Hemalatha, P., Elumalai, D., Janaki, A., Babu, M., Velu, K. and Velayutham, K. 2015. Larvicidal activity of Lantana camara aculeata against three important mosquito species. Journal of Entomology and Zoology Studies, 3: 174-181.
Hillary, V.E., Ceasar, S.A. and Ignacimuthu, S. 2024. Efficacy of plant products in controlling disease vector mosquitoes: A review. Entomologia Experimentalis et Applicata, 172: 195-214. Isman, M.B. and Machial, C.M. 2006. Pesticides based on plant essential oils: from traditional practice to commercialization. Advances in Phytomedicine, 3: 29-44.
Jundare, P., Kshirsagar, S., Kandalkar, A. and Jaiswar, S. 2020. Study on Parthenium hysterphorous for mosquitoes larvicidal potential and the formulation of larvicidal sachet. American Journal of PharmTech Research, 10: 93-102.
Kumar, P., Shakya, R., Kumar, V., Kumar, D., Chauhan, R. and Singh, H. 2023. Chemical constituents and strong larvicidal activity of Solanum xanthocarpum among selected plants extracts against the malaria, filaria and dengue vectors. Journal of Vector Borne Diseases, 60: 18-31.
Kumar, S. and Kumar, S. 2024. Assessment of larvicidal efficacy of tropical plants Parthenium hysterophorus, hyptis suaveolens and Mentha arvensis aqueous leaf extracts against mosquito Aedes aegypti. World Journal of Pharmaceutical Research, 13: 611-624.
Kumar, S., Kapinder, and Kumar, S. 2021. Evaluation of mosquito larvicidal potential of indigenous plants of eastern Uttar Pradesh. pp. 43-48. In: Proceedings of the Present Status and Future Trends in Entomological and Wildlife Studies. July, 13-14, 2021, Himanshu Publications, Mohanlal Sukhodia University, Udaipur, Rajasthan.
Lakshmi, K.V., Sudhikumar, A.V. and Aneesh, E.M. 2018. Larvicidal activity of phytoextracts against dengue fever vector, Aedes aegypti - A review. Plant Science Today, 5: 167-174. Mdoe, F.P., Cheng, S.S., Msangi, S., Nkwengulila, G., Chang, S.T. and Kweka, E.J. 2014. Activity of Cinnamomum osmophloeum leaf essential oil against Anopheles gambiae. Parasites & Vectors, 7: 1-2.
Obomanu, F.G., Ogbalu O.K., Gabriel, U.U., Fekarurhobo, G.K. and Adediran, B.I. 2006. Larvicidal properties of Lepidagathis alopecuroides and Azadirachta indica on Anopheles gambiae and Culex quinquefasciatus. African Journal of Biotechnology, 5: 761-765.
Peeyush, K., Mishra, S., Malik, A. and Satya, S. 2011. Insecticidal properties of Mentha species: A review, Industrial Crops and Products, 34: 802-817.
Redfern, J., Kinninmonth, M., Burdass, D. and Verran, J. 2014. Using Soxhlet ethanol extraction to produce and test plant material (essential oils) for their antimicrobial properties. Journal of Microbiology & Biology Education, 15: 45-46.
Reichardt, C. and Welton, T. 2010. Solvents and Solvent Effects in Organic Chemistry, John Wiley & Sons, Weinheim, Germany.
Ricciutelli, M., Di Martino, P., Barboni, L. and Martelli, S. 2006. Evaluation of rapamycin chemical stability in volatile-organic solvents by HPLC. Journal of Pharmaceutical and Biomedical Analysis, 41: 1070-1074.
Robertson, J.L. Russell R.M., Preisler, H.K. and Savin, N.E. 2007. Bio-assays with Arthropods: A POLO Computer Program. Taylor and Francis/ CRC Press, Boca Raton, Florida, USA. Rueda, LM. 2004. Pictorial keys for the identification of mosquitoes (Diptera: Culicidae) associated with dengue virus transmission. Zootaxa, 589(1): 1-60.
Sanjeev, K. and Sushil, K. 2022. Efficacy of phytochemicals against mosquito larvae: An update to integrated mosquito management. International Journal of Zoological Investigations, 8: 305-319. Sarita, K., Nair, G., Singh, A.P., Batra, S., Wahab, N. and Warikoo, R. 2022. Evaluation of the larvicidal efficiency of stem, roots and leaves of the weed, Parthenium hysterophorus (family: Asteraceae) against Aedes aegypti L. Asian Pacific Journal of Tropical Diseases, 2: 395-400., Selvi, V., Arivoli, S. and Tennyson, S. 2024. Toxic effect of green seaweeds on the larval instars of vector mosquitoes. Advances in Zoology and Botany, 12: 63-75.
Shaalan, E.A.S., Canyon, D., Younes, M.W.F., Abdel-Wahab, H. and Mansour, A.H. 2005. A review of botanical phytochemicals with mosquitocidal potential. Environment International, 31: 1149- 1166.
Shinde, L., Goyal, M., Bayas, R. and Patil, S. 2018. Review on potential eco-friendly biolarvicides against dengue vector: Aedes aegypti (Linn) (Diptera: Culicidae). International Journal of Life Sciences Research, 6: 61-80.
Srivastava, A., Bartaya, R., Tonks, S., Srivastava, S.S. and Kumari, M. 2008. Larvicidal activity of an indigenous plant, Centratherum anthelminticum. Journal of Environmental Biology, 29: 669- 672.
Tarekegn, M., Wolde-Hawariat, Y., Dugassa, S. and Tekie, H. 2021. Evaluation of larvicidal activities of Parthenium hysterophorus L. against Anopheles arabiensis (Diptera: Culicidae), the major malaria vector in Ethiopia. International Journal of Tropical Insect Science, 41: 1461-1499.
WHO. 2017. Global Diffusion of eHealth: Making Universal Health Coverage Achievable: Report of the Third Global Survey on eHealth. Geneva, Switzerland.
WHO. 2022. Laboratory Testing for Zika Virus and Dengue Virus Infections: Interim Guidance. 14 July 2022. WHO, Geneva, Switzerland.
WHO. 2022. WHO Guidelines for Malaria. 3 June 2022. WHO, Geneva, Switzerland. WHO. 2024. Disease Outbreak News, Dengue - Global Situation. [https://www.who.int/ emergencies/ disease-outbreak-news/item/2024-DON518].
WHO. Year? Guidelines for Laboratory and Field Testing of Mosquito Larvicides. World Health Organization, Geneva, Switzerland. [WHO_CDS_WHOPES_GCDPP.13.pdf, 2005-2015]. Yang, T., Lu, L., Fu, G., Zhong, S. and Ding, G. 2009. Epidemiology and vector efficiency during a dengue fever outbreak in Cixi,Zhejiang province,China. Journal of VectorEcology, 34: 148-154. Yu, K.X., Jantan, I., Ahmad, R. and Wong, C.L. 2014. The major bioactive components of seaweeds and their mosquitocidal potential. Parasitology Research, 113: 3121–3141.