BIOEXTRACTION OF IRON THROUGH Bacillus sp. SP10 FROM MINERAL  OXIDE AND ITS APPLICATION AS PLANT MICRONUTRIENT

M AMary Deva Prasanna; K Gokulraj; S Rajakumar; PM Ayyasamy

doi:10.48165/abr.2024.26.01.15

Authors

M AMary Deva Prasanna Department of Microbiology, Periyar University, Salem – 636 011, Tamil Nadu (India)
K Gokulraj Department of Microbiology, Periyar University, Salem – 636 011, Tamil Nadu (India)
S Rajakumar Department of Marine Biotechnology, Bharathidasan University, Tiruchirappalli – 620 024, Tamil Nadu (India)
PM Ayyasamy Department of Microbiology, Periyar University, Salem – 636 011, Tamil Nadu (India)

DOI:

https://doi.org/10.48165/abr.2024.26.01.15

Keywords:

Bacillus sp, column study, ferric hydroxide, iron mineralization

Abstract

Iron is an essential micronutrient for all living organisms because of its critical role in metabolic processes. In plants it plays a key role in the synthesis of chlorophyll, some vital enzymatic and metabolic processese. However, most of the iron in nature is present as precipitates or in insoluble forms. Therefore, biological mechanism has been employed to mineralize Fe(III) oxides into Fe(II) through microbial action to increase the availability of soluble iron. An iron reducing bacterial strain was isolated based on its tolerant limits and iron mineralizing ability. The strain Bacillus sp. SP10 effectively mineralized iron in synthetic medium possessing 1% starch and pH 7. In a 25 day’s column study, the soluble Fe(II) as micronutrient showed a gradual increase in each seepage collected from different treatment columns. Similarly, the plants grown on soil obtained from treatment D (i.e. 1% starch + 1% Bacillus sp. SP10 inoculum + 0.25% anthraquinone-2,6-disulphonate) column displayed good growth and maximum shoot length, mainly due to the increased accessibility of iron through microbial mineralization. The mineralization of Fe in soil was established through SEM and EDX analysis.

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References

Ahemad, M. 2014. Remediation of metalliferous soils through the heavy metal resistant plant growth promoting bacteria: paradigms and prospects. Arabian Journal of Chemistry, 12: 1365-1377. Ahuti, S. 2015. Industrial growth and environmental degradation. International Education and Research Journal, 1(5): 1-3.

Asha, L.P. and Sandeep, R.S. 2013. Review on bioremediation - Potential tool for removing environ mental pollution. International Journal of Basic and Applied Chemical Science, 3(3): 21-33. Ayyasamy, P.M., Chun, S. and Lee, S. 2009. Desorption and dissolution of heavy metals from contaminated soil using Shewanella sp. (HN-41) amended with various carbon sources and synthetic soil organic matters. Journal of Hazardous Materials, 161: 1095-1102. Ayyasamy, P.M. and Lee, S. 2009. Redox transformation and biogeochemical interaction of heavy metals in Korean soil using different treatment columns in the presence of Shewanella sp. Chemosphere, 77(4): 501-509.

Ayyasamy, P.M. and Lee, S. 2012. Biotransformation of heavy metals from soil in synthetic medium enriched with glucose and Shewanella sp. HN-41 at various pH. Geomicrobiology Journal, 29(9): 843-851.

Bond, D.R. and Lovley, D.R. 2002. Reduction of Fe(III) oxide by methanogens in the presence and absence of extracellular quinines. Environmental Microbiology, 4: 115-124. Bonneville, S., Cappellen, P.V. and Behrends, T. 2004. Microbial reduction of iron (III) oxyhydroxides: Effects of mineral solubility and availability. Chemical Geology, 212: 255-268. Buchanan, R.E. and Gibbons, N.E. 1974. Bergey's Manual of Determinative Bacteriology (8th edn.). Williams & Wilkins Co., Baltimore, Madison, USA.

Byrne, R.H., Luo, Y.R. and Young, R.W. 2000. Iron hydrolysis and solubility revisited: Observations and comments on iron hydrolysis characterizations. Marine Chemistry, 70: 23-35.

Bioextraction of iron from synthetic mineral oxide 109

Chakraborty, S.P., Mahapatra, S.K. and Roy, S. 2011. Biochemical characters and antibiotic susceptibility of Staphylococcus aureus isolates. Asian Pacific Journal of Tropical Biomedicine, 1(3): 212-216.

Garbisu, C. and Alkorta, I. 2001. Phytoextraction: A cost-effective plant-based technology for the removal of metals from the environment. Bioresource Technology, 77: 229-236. Harry Pyenson and Tracy, P.H. 1945. A 1,10-phenanthroline method for the determination of iron in powdered milk. Journal of Dairy Science, 28: 401-412.

Kanwal, R., Ahmed, T., Tahir, S.S. and Rauf, N. 2004. Resistance of Bacillus cereus and E. coli towardslead, copper, iron,manganese and arsenic.Pakistan Journal ofBiological Sciences,7: 6-9. Lovley, D.R., Philips, E.J.P., Gorby, Y.A. and Landa, E.R. 1991. Microbial reduction of uranium. Nature, 350: 413-416.

Lovley, D.R. and Phillips, E.J.P. 1988. Novel mode of microbial energy metabolism: Organic carbon oxidation to dissimilatory reduction of iron or manganese. Applied and Environmental Microbiology. 54(6): 1472-1480.

Ma, J.F. and Ling, H.Q. 2009. Iron for plants and humans. Plant Soil, 325(1-2): 1-3. Man, Y., Xu, T., Adhikari, B., Zhou, C., Wang, Y. and Wang, B. 2021. Iron supplementation and iron-fortified foods: A review. Critical Reviews in Food Science and Nutrition, 62: 16. [https://doi.org/10.1080/10408398.2021.1876623].

Mgbemene, C.A., Nnaji, C.C. and Nwozor, C. 2016. Industrialization and its backlash: Focus on climate change and its consequences. Journal of Environmental Science and Technology, 9(4): 301-316.

Nikolic, M. 2018. Plant responses to iron deficiency and toxicity and iron use efficiency in plants. Plant Micronutrient Use Efficiency, 2018: 55-69.

Pierre, J.L., Fontecave, M. and Crichton, R.R. 2002. Chemistry for an essential biological process: The reduction of ferric iron. Bio Metals, 15: 341-346.

Rickard, D. 2012. Sedimentary iron Biochemistry. pp. 85-119. In: Developments in Sedimentology, Vol. 65 [https://doi.org/10.1016/B978-0-444-52989-3.00003-9].

Romheld, V. and Nikolic, M. 2006. Iron. pp. 329-350. In: Handbook of Plant Nutrition (eds. A.V. Baker and D.J. Pilbeam), CRC Press, Boca Raton Florida, USA.

Santos, E.O. and Martins, M.L.L. 2003. Effect of the medium composition on formation of amylase by Bacillus sp. Brazilian Archives of Biology and Technology, 46: 129-134.

Santos, H.F., Carmo, F.L., Paes, J.E.S., Rosado, A.S. and Peixoto, R.S. 2011. Bioremediation of mangroves impacted by petroleum. Water, Air and Soil Pollution, 216: 329-350. Saryono, Finna, P., Usman, P., Wahyu, P.N. and Aulia, A. 2019. Isolation and identification of bacteria and actinomycetes isolated from wilting banana plants (Musa sp.). Materials Science and Engineering, 532-012028. [DOI 10.1088/1757-899X/532/1/012028].

Sivakami, G., Priyadarshini, R., Baby, V., Rajakumar, S. and Ayyasamy, P.M. 2012. Bioremediation of ferric iron in synthetic metal oxide using Bacillussp. (SO-10). Journal of Current Perspectives in Applied Microbiology, 1(2): 34-41.

Sun, Y., Wang, W., Zheng, F., Zhang, S., Wang, F. and Liu, S. 2020. Phytotoxicity of iron-based materials in mung bean: Seed germination test. Chemosphere, 251: 126432. [https://doi.org/10.1016/j.chemosphere.2020.126432].

Valko, M., Jomova, K., Rhodes, C.J., Kuca, K. and Musilek, K. 2016. Redox and non-redox metal induced formation offree radicals and theirrole in human disease.Archives ofToxicology, 90: 1-37. Vargas, M., Kashefi, K., Blunt-Harris, E.L. and Lovley, D.R. 1998. Microbiological evidence for Fe(III) reduction on early earth. Nature, 395: 65-67.

Verma, S. and Kuila, A. 2019. Bioremediation of heavy metals by microbial process. Environmental Technology & Innovation, 14: 100369. [https://doi.org/10.1016/j.eti.2019.100369]. Yi, W., Wang, B. and Qu, D. 2012. Diversity of isolates performing Fe(III) reduction from paddy soil fed by different organic carbon sources. African Journal of Biotechnology, 11(19): 4407-4417.