In vitro ANALYSIS OF BIOFILM FORMATION AND ANTIBIOTIC RESISTANCE OF UROPATHOGENIC Escherichia coli
DOI:
https://doi.org/10.48165/abr.2024.26.01.5Keywords:
Antibiotic resistance, biofilm, uropathogenic Escherichia coli, urinary tract infectionAbstract
Urinary tract infections are an exceedingly common worldwide problem, caused mostly by Gram-negative bacteria, especially Escherichia coli. Microbial biofilms are considered a serious public health problem. The potential of uropathogenic E. coli (UPEC) to produce biofilm was explored in the present study. The Congo red agar, tube- and tissue culture plate methods were used to evaluate the formation of biofilm by E. coli isolates. Of the 155 isolates, 101, 106 and 90 isolates were positive for these three methods, respectively. Subsequently, the sensitivity of E. coli isolates to antimicrobial agents (amikacin, cefepime, cefixime, cefotaxime, cefpodoxime, ceftazidime, ceftriaxone, ciprofloxacin, nitrofurantoin, gentamycin, nalidixic acid, ofloxacin and pefloxacin) was tested. There was no difference in the rate of biofilm detection between Congo red agar method and tube method. The antibiotic sensitivity test revealed that the biofilm producing isolates were multi-drug resistant. The study emphasized the necessity for developing alternative therapeutic approaches to overcome multi drug resistance arising from biofilm formation of UPEC.
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Arafa, S.H., Alshehri, W.A., Organji, S.R., Elbanna, K., Obaid, N.A., Aldosari, M.S., Asiri, F.H., Ahmad, I. and Abulreesh, H.H. 2022. Antimicrobial resistance, virulence factor-encoding genes, and biofilm-forming ability of community-associated uropathogenic Escherichia coli in Western Saudi Arabia. Polish Journal of Microbiology, 71(3): 325-339.
Behzadi, P., Urbán, E. and Gajdács, M. 2020. Association between biofilm-production and antibiotic resistance in uropathogenic Escherichia coli (UPEC): An in vitro study. Diseases, 8(2): 17. [https://doi.org/10.3390/diseases8020017].
Bekő, K., Nagy, E.Z., Grózner, D., Kreizinger, Z. and Gyuranecz, M. 2022. Biofilm formation and its impact on environmental survival and antibiotic resistance of Mycoplasma anserisalpingitidis strains. Acta Veterinaria Hungarica, 70(3): 184-191.
Bhando, T., Dubey, V. and Pathania, R. 2019. Biofilms in antimicrobial activity and drug resistance. pp. 109-139. In: Bacterial Adaptation to Co-resistance (eds. S. Mandal, and D. Paul). Springer, Singapore. [https://doi.org/10.1007/978-981-13-8503-2_6].
Cetin, N., Gencler, A. and Kavaz Tufan, A. 2020. Risk factors for development of urinary tract infection in children with nephrolithiasis. Journal of Paediatrics and Child Health, 56(1): 76-80. Chen, X.P., Ali, L., Wu, L.Y., Liu, C., Gang, C.X., Huang, Q.F., Ruan, J.H., Bao, S.Y., Rao, Y.P. and Yu, D. 2018. Biofilm formation plays a role in the formation of multidrug-resistant Escherichia coli toward nutrients in microcosm experiments. Frontiers in Microbiology, 9: [https://doi.org/10.3389/fmicb.2018.00367].
Clinical and Laboratory Standards Institute (CLSI). 2016. Performance Standards for Antimicrobial Susceptibility Testing. 26th Informational supplement; CLSI, Wayne, Pennsylvania, USA. Dash, D., Sarangi, G., Patro, P. and Chayani, N. 2018. Study of biofilm production in EIscherichia coli causing urinary tract infection and its correlation with antimicrobial resistance. Journal of the Academy of Clinical Microbiologists, 20(2): 88-91.
Goudarztalejerdi, A., Mohammadzadeh, A., Niazi, K. and Mohammad Mirzaei, M. 2022. High prevalence of multidrug resistance and biofilm-formation ability among avian Escherichia coli isolated from broilers in Iran. Microbial Drug Resistance (Larchmont, N.Y.), 28(2): 244-254.
Haji, S.H. 2018. Detection of biofilm formation in Pseudomonas aeruginosa isolates from clinical specimens. Zanco Journal of Pure and Applied Sciences, 30(4): 83-89.
Jamal, M., Ahmad, W., Andleeb, S., Jalil, F., Imran, M., Nawaz, M.A., Hussain, T., Ali, M., Rafiq, M. and Kamil, M.A. 2018. Bacterial biofilm and associated infections. Journal of the Chinese Medical Association, 81(1): 7-11.
Javed, S., Mirani, Z.A. and Pirzada, Z.A. 2020. Phylogenetic group B2 expressed significant biofilm formation among drug resistant uropathogenic Escherichia coli. Libyan Journal of Medicine, 16(1): 1845444. [https://doi.org/10.1080/19932820.2020.184544].
Karigoudar, R., Karigoudar, M.H., Wavare, S.M. and Mangalgi, S.S. 2019. Detection of biofilm among uropathogenic Escherichia coli and its correlation with antibiotic resistance pattern. Journal of Laboratory Physicians, 11(01): 17-22.
Kwok, T.Y., Ma, Y. and Chua, S.L. 2022. Biofilm dispersal induced by mechanical cutting leads to heightened food borne pathogen dissemination. Food Microbiology, 102: 103914. [https://doi.org/10.1016/j.fm.2021.103914].
Mittal, S., Sharma, M. and Chaudhary, U. 2015. Biofilm and multidrug resistance in uropathogenic Escherichia coli. Pathogens and Global Health, 109(1): 26-29.
Mohsenzadeh, A., Fazel, A., Bavari, S., Borji, S., Pourasghar, S., Azimi, T. and Sabati, H. 2021. Detecting of biofilm formation in the clinical isolates of Pseudomonas aeruginosa and
Biofilm formation and antibiotic resistance of E. coli 7
Escherichia coli: An evaluation of different screening methods. Journal of Current Biomedical Reports, 2(2): 56-61.
Muhammad, Z.A., Rao, T., Ahmad, W., Shahzeb, H, Samrana, A., Asma, A., Wafa, I., Muhammad, Y., Ali, A., Ojha, S.C., Liaqat, A., Naseer, R., Shehla Munir, Muhammad, I. and Sarfraz, A. 2021. pp.33-56. In: Biochemistry of Drug Resistance (eds. Ahmed, S., Ojha, C.S., Najam-ul Haq, M., Younus, M., Hashmi, M.Z.) Springer, Cham. [https://doi.org/10.1007/978-3-030- 76320-6_2].
Novais, ., Vuotto, C., Pires, J., Montenegro, C., Donelli, G., Coque, T.M. and Peixe, L. 2013. Diversity and biofilm-production ability among isolates of Escherichia coli phylogroup D belonging to ST69, ST393 and ST405 clonal groups. BMC Microbiology, 13(1): 144 [https://doi.org/10.1186/1471-2180-13-144].
O’Toole, G.A. 2011. Microtiter dish biofilm formation assay. Journal of Visualized Experiments, 47: 2437 [https://doi.org/10.3791/2437].
Panda, P.S., Chaudhary, U. and Dube, S.K. 2016. Comparison of four different methods for detection of biofilm formation by uropathogens. Indian Journal of Pathology and Microbiology, 59: 177- 179.
Ponnusamy, P., Vidhya, N. and Sevanan, M. 2012. In vitro biofilm formation by uropathogenic Escherichia coli and their antimicrobial susceptibility pattern. Asian Pacific Journal of Tropical Medicine, 5(3): 210-213.
Qian, W., Li, X., Yang, M., Liu, C., Kong, Y., Li, Y., Wang, T. and Zhang, Q. 2022. Relationship between antibiotic resistance, biofilm formation, and biofilm-specific resistance in Escherichia coli isolates from Ningbo, China. Infection and Drug Resistance, 15: 2865-2878.
Risal, G., Shrestha, A., Kunwar, S., Paudel, G., Dhital, R., Budha, M.B. and Nepal, R. 2018. Detection of biofilm formation by Escherichia coli with its antibiogram profile. International Journal of Community Medicine and Public Health, 5(9): 3771-3775.
Schmalz, G. and Cieplik, F. 2021. Biofilms on restorative materials. Monographs in Oral Science, 29: 155-194.
Sutton, S. 2011. Determination of inoculum for microbiological testing. Journal of GXP Compliance, 15(3): 49-53.
Siddhiqui, S., Afreen, U. and Kotgire, S. 2018. Evaluation of biofilm formation by three different methods and its antibiogram with special reference to indwelling medical devices from a tertiary care hospital. Annals of Pathology and Laboratory Medicine, 5(2): 171-176.
Sharma, D., Misba, L. and Khan, A.U. 2019. Antibiotics versus biofilm: An emerging battle ground in microbial communities. Antimicrobial Resistance and Infection Control, 8(1): 76. [https://doi.org/10.1186/s13756-019-0533-3].
Sultan, A.M. and Nabiel, Y. 2019. Tube method and Congo red agar versus tissue culture plate method for detection of biofilm production by uropathogens isolated from midstream urine: Which one could be better? African Journal of Clinical and Experimental Microbiology, 20(1): 60-66.
Tajbakhsh, E., Ahmadi, P., Abedpour-Dehkordi, E., Soleimani, N.A. and Khamesipour, F. 2016. Biofilm formation, antimicrobial susceptibility, serogroups and virulence genes of uropathogenic E. coli isolated from clinical samples in Iran. Antimicrobial Resistance and Infection Control, 5(1): [https://doi.org/10.1186/s13756-016-0109-4].
Zheng, S., Bawazir, M., Dhall, A., Kim, H.E., He, L., Heo, J. and Hwang, G. 2021. Implication of surface properties, bacterial motility, and hydrodynamic conditions on bacterial surface sensing and their initial adhesion. Frontiers in Bioengineering and Biotechnology, 12(9): 643722. [https://doi.org/10.3389/fbioe.2021.643722].
Zhou, Z.J., Sun, B.G. and Sun, L. 2015. Edwardsiella tarda Sip1: A serum-induced zinc metalloprotease that is essential to serum resistance and host infection. Veterinary Microbiology, 177(3-4): 332-340.
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