Influence Of Iron On Phosphate Metabolism In Cyanobacterium Nostoc Calcicola
DOI:
https://doi.org/10.48165/Keywords:
Alkaline phosphatase, cyanobacterium, Nostoc calcicola, phosphate, ATPaseAbstract
The phosphate uptake, alkaline phosphates and total extractable ATP-pool were investigated in a cyanobacterium Nostoc calcicola Breb. under iron stress conditions. The phosphate uptake was maximum in cells raised on 200 µM Fe3+ supplemented Allen and Arnon medium, whereas 150 µM Fe3+ (used for routine culture of Nostoc calcicolas) supported lower phosphate uptake. The cells supplemented with higher iron (300-500 µM Fe3+) depicted reduction in phosphate uptake with maximum reduction observed in 500 µM Fe3+ supplemented cells. Similar results were observed with respect to the effect of iron on alkaline phosphatase activity. The alkaline phosphatase activity was minimum in cells raised on medium having 500 µM Fe3+. Also, the increase in ATPase activity showed corresponding reduction in total extractable ATP-pool. The reduction in ATP-pool is the resultant of lower phosphate level in cells under Fe3+ stress.
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Allen, M.B. and Arnon, D.I. 1955. Studies on nitrogen fixing blue green algae: Growth and nitrogen fixation by Anabaena cylindrica. Lemm. Plant and Cell Physiology, 30: 366-370. Applebury, M.E., Johnson, B.P. and Coleman, J.E. 1970. Phosphate binding to alkaline phosphatase. Journal of Biological Chemistry, 245: 4968-4975.
Asthana, R.K., Pandey, P.K. and Singh, S.P. 1990. Nickel regulation of photoautotrophy in a cyanobacterium. Water, Air and Soil Pollution, 52: 263-276.
Batterton, J.C. and Van Baalen. 1996. Phosphorus deficiency and phosphate uptake in the blue green algae Anacystis nidulans. Canadian Journal of Microbiology, 14: 341-348.
Bottomley, P.J. and Stewart, W.D.P. 1976. ATP pools and transients in the blue green algae Anabaena cylindrica. Archives in Microbiology, 108: 249-258.
Falkner, G., Horner, F. and Simonis, W. 1980. The regulation of the energy dependent phosphate uptake by the blue green algae Anacystis nidulans. Planta, 149: 562-569.
Fay, P. 1969. Matabolic activities of isolated spores of Anabaena cylindrical. Journal of Experimental Biology, 20: 100-109.
Fiske, C.H. and Subba Row. 1925. Colorimetric determination of phosphorus .Journal of Biological Chemistry, 66: 375-400.
Grillo, J.F. and Gibson, J. 1979. Regulation of phosphate accumulation in the unicellular cyanobacterium Synechococcus. Journal of Bacteriology, 140: 508-517.
Healey, F.P. 1973. Characteristics of phosphorus deficiency in Anabaena. Journal of Phycology, 9: 383-390.
Healey, F.P. 1982. Phosphate in the biology of cyanobacteria (Eds. N.G. Carr and B.A. Whitton). Blackwell Scientific Publication, Oxford, London.
Ihlenfeldt, M.J.A. and Gibson, J. 1975. Phosphate utilization and phosphate activity in Anacystis nidulans (synechococcus). Archives in Microbiology. 102: 23-28.
Larrson, C.M. and Olsson, T. 1979. Firefly assay of adenine nucleotide from algae: Comparison of extraction methods. Plant and Cell Physiology, 20: 145-155.
Lockau, W. and Pfeffer, S. 1982. A cyanobacterial ATPase distinct from the coupling factor of photophosphorylation. Z. Naturofersch. 37: 658-664.
Nalewajako, C. and Paul, B. 1985. Effect of manipulations of aluminum concentrations and pH on the phosphate uptake and photosynthesis of planktonic communities of two pre-Cambrian shield lakes. Canadian Journal of Fish and Aquatic Science, 42: 1946-1953.
Pandey, P.K., Singh, C.B. and Singh, S.P. 1992. Copper uptake in a cyanobacterium: Fate of selected photochemical reactions . Current Microbiology. 42: 332-335.
Petterson, A., Hallbom, L. and Bergman, B. 1988. Aluminum effect on uptake and metabolism of phosphate in Anabaena cylindrica. Plant Physiology, 86: 112-116.
Price, N.M. and Morell, F.M.M. 1990. Cadmium and cobalt substitution for zinc in marine diatoms. Nature, 344: 568-660.
Rai, L.C., Tyagi, B., Mallick, N., Husaini, Y., Singh, A.K. and Dubey, S.K. 1990. Effect of four heavy metals on the biology of Nostoc muscorum. Bio- metals, 2: 229-234.
Ross, R.D., Cooke, R.S., Burden, D.S. and James, C.S. 1990. Effect of herbicide MCPA, and the heavy matels, cadmium and nickel on the lipid composition, Mg ATPase activity and fluidity of plasma membranes from rice Oryza sativa (cv. Bahia) shoots. Journal of Experimental Botany, 41: 457- 462.
Ructer, J.G., Mecarty, J.J. and Carpenter, E.J. 1979. Toxic effects of copper on Oscillatoria (Trichodesmium) theibanti. Limnology and Oceanography, 24: 558-562.
Singh, S.P. and Yadava, V. 1984. Cadmium uptake in Anacystis nidulans: Effect of modifying factor. Journal of General and Applied Microbiology, 31: 39-48.
