Status Of Apple Mosaic Virus In Kashmir Valley

B A Padder; M D Shah; Mushtaq Ahmad; Aflaq Hamid; T A Sofi; F A  Ahanger; Sahar Saleem

doi:10.48165/

Authors

B A Padder Division of Plant Pathology, S.K. University of Agricultural Sciences & Technology of Kashmir, Shalimar, Srinagar, Jammu & Kashmir 191 121, India
M D Shah Division of Plant Pathology, S.K. University of Agricultural Sciences & Technology of Kashmir, Shalimar, Srinagar, Jammu & Kashmir 191 121, India
Mushtaq Ahmad Division of Plant Pathology, S.K. University of Agricultural Sciences & Technology of Kashmir, Shalimar, Srinagar, Jammu & Kashmir 191 121, India
Aflaq Hamid Division of Plant Pathology, S.K. University of Agricultural Sciences & Technology of Kashmir, Shalimar, Srinagar, Jammu & Kashmir 191 121, India
T A Sofi Division of Plant Pathology, S.K. University of Agricultural Sciences & Technology of Kashmir, Shalimar, Srinagar, Jammu & Kashmir 191 121, India
F A Ahanger Division of Plant Pathology, S.K. University of Agricultural Sciences & Technology of Kashmir, Shalimar, Srinagar, Jammu & Kashmir 191 121, India
Sahar Saleem Division of Plant Pathology, S.K. University of Agricultural Sciences & Technology of Kashmir, Shalimar, Srinagar, Jammu & Kashmir 191 121, India

DOI:

https://doi.org/10.48165/

Keywords:

Apple mosaic virus, ApMV incidence, Kashmir, RT-PCR detection

Abstract

Leaves of apple cv. ‘Red Delicious’ and ‘Golden Delicious’ were randomly collected from commercial apple growing areas of four districts of Jammu and Kashmir State viz., Shopian, Pulwama, Bandipora and Kupwara for the detection of Apple mosaic virus (ApMV) by RT-PCR technique using coat protein gene-specific primers. ApMV was present in all the locations. RT-PCR amplified approximately 800 bp amplicons from 30 out of 276 leaf samples thereby confirmed the infection of ApMV in Kashmir. District Bandipora recorded highest disease incidence of 13.3% followed by Shopian (11.3%), Kupwara (10.2%) and Pulwama (8.6%). Nineteen of the 161 cv. ‘Red Delicious’ samples and 11 of the 115 ‘Golden Delicious’ samples were found RT-PCR positive.

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References

Bhargava, K.S. and Bist, N.S. 1957. Three virus diseases of hill fruits in Kumaon. Current Science, 26: 324–325.

Helguera, P.R., Docampo, D.M., Nome, F. and Ducasse, D.A. 2002. Enhanced detection of Prune dwarf virus in peach leaves by immunocapture-reverse transcription-polymerase chain reaction with nested polymerase chain reaction (IC-RT-PCR nested PCR). Journal of Phytopathology, 150: 94-96.

Mink, G. I. 1989. Apple chlorotic leafspot. pp. 8–19; 34-39 In: Virus and Virus-like Diseases of Pome Fruits and Simulating Non-infectious Disorders (eds. P.R. Fridlund). Pullman, USA/ Cooperative Extension College of Agriculture and Home Economics, Washington State University, Washington, USA.

B.A. Padder et al. 120

Moury, B., Cardin, L., Onesto, J.P., Candresse, T. and Poupet. A. 2000. Enzyme-linked immunosorbent assay testing of shoots grown in vitro and the use of immunocapture-reverse transcription polymerase chain reaction improve the detection of prunus necrotic ringspot virus in rose. Phytopathology, 90: 522-528.

Rana, T., Chandel, V., Kumar, Y., Ram, R., Hallan, V. and Zaidi, A.A. 2010 Molecular variability analyses of Apple chlorotic leaf spot virus capsid protein. Journal of Bioscience, 35: 605–615. Rwahnih, M.A., Turturo, C., Minafra, A., Saldarelli, P., Myrta, A., Pallás, V. and Savino, V. 2004. Molecular variability of apple chlorotic leaf spot virus in different hosts and geographical regions. Journal of Plant Pathology, 86: 117-122.

Salmon, M.A., Vendrame, M., Kummert, J. and Lepoivre, P. 2002. Detection of apple chlorotic leaf spot virus using 5’ nuclease assay with a fluorescent minor groove binder-DNA probe. Journal of Virological Methods, 104: 99-106.

Sutic, D.D., Ford, R.E., and Tosic, M.T. 1999. Virus diseases of fruit trees. pp. 345–347. In: Handbook of Plant Virus Diseases. CRC Press, Boca Raton, Florida, USA.

Thokchom, T., Rana, T., Hallan, V., Raja Ram and Zaidi, A.A. 2009. Molecular characterization of the Indian strain of Apple mosaic virus isolated from apple (Malus domestica). Phytoparasitica, 37: 375-379.

Ulubas, C. and Ertunc, F. 2005. Apple chlorotic leaf spot virus (ACLSV) status in Turkey and sensitive detection using advanced techniques. Turkish Journal of Agricultural Forestry, 29: 251-257.