First Report Of Dpp-Iii In Plants: Its Subcellular  Localization In Germinating Mung Bean (Vigna Radiata) Seeds  And Distribution In Plant Parts

Drukshakshi Jodha; Pooja Attri; Tejinder P Khaket; Jasbir Singh

doi:10.48165/

Authors

Drukshakshi Jodha Department of Biochemistry, Kurukshetra University, Kurukshetra, Haryana - 136 119 (India)
Pooja Attri Department of Biochemistry, Kurukshetra University, Kurukshetra, Haryana - 136 119 (India)
Tejinder P Khaket Department of Biochemistry, Kurukshetra University, Kurukshetra, Haryana - 136 119 (India)
Jasbir Singh Department of Biochemistry, Kurukshetra University, Kurukshetra, Haryana - 136 119 (India)

DOI:

https://doi.org/10.48165/

Keywords:

Arg-Arg-4-mβNA, cytosolic, DPP-III, germinating seeds Vigna radiata

Abstract

Arginyl-arginyl-4-methoxy-β-naphthylamide (Arg-Arg-4mβNA) hydro lyzeing activity is known as dipeptidylpeptidase-III (DPP-III). DPP-III enzyme hydrolyzes various bioactive peptides, including enkephalins, angiotensins, gastric peptides and Arg-Arg-4mβNA. We report the presence of DPP-III enzyme in plants for the first time. The enzyme was noticed in mung bean [Vigna radiata] seeds germinated at 25ºC for 48 h. The crude enzyme preparation had optimum activity at alkaline pH of 8.0. Sub-cellular distribution of this enzyme revealed its cytosolic localization. Enzyme activity when investigated in different parts of mung bean plant revealed maximum activity in seeds followed by roots, stem and leaves. The ubiquitous distribution of this enzyme signifies house keeping role of this enzyme in plant physiology. Maximum activity in seeds indicates its possible role in germination.

Downloads

Download data is not yet available.

References

Caldwell, J.B. and Sparrow, L.G. 1976. Partial purification and characterization of two peptide hydrolases from pea seeds. Plant Physiology, 57: 795-798.

Chan, S.A., Toursarkissian, K., Sweeney, J.P. and Jones, T.H. 1985. Dipeptidyl aminopeptidases and aminopeptidases in Dictyostelium discoideum. Biochemical and Biophysical Research Communication, 127: 962-968.

Davy, A., Thomsen, K.K., Juliano, M.A., Alves, L.C., Svendsen, I. and Simpson, D. 2000. Purification and characterization of barely dipeptidyl peptidase IV. Plant Physiology, 122: 425-431. Dhanda, S. and Singh, H. 2010. Distribution of dipeptidylpeptidase III homologue in brain and vital organ in goat (Capra hircus). Indian Journal of Animal Sciences, 79: 715-718.

Dhanda, S., Singh, H., Singh, J. and Singh, T.P. 2007. Isolation purification and characterization of a DPP-III homologue from goat brain. Protein Expression and Purification, 52: 297-305. Ellis, S. and Nuenke, J.M. 1967. Dipeptidyl arylamidase III of the pituitary: Purification and characterization. Journal of Biological Chemistry, 242: 4623-4629.

MacDonald, J.K. and Barrett, A.J. 1986. Mammalian Proteases: A Glossary and Bibliography, Volume 2. Academic Press, London, UK.

Mazzocco, C., Fukasawa, K.M., Auguste, P. and Puiroux, J. 2003. Characterization of functionally expressed dipeptidyl aminopeptidase III from Drosophila melanogaster. European Journal of Biochemistry, 270: 3074-3082.

Ohkubo, I., Yao-Hua, L., Maeda, T., Yamamoto, Y., Yamane, T., Du, P.G. and Nishi, K. 2000. Molecular cloning and immunohistochemical localization of rat dipeptidylpeptidase III. Forensic Science International, 113: 147-151.