Molecular And Antibody Based Diagnosis Of Bovine Viral  Diarrhea Virus (Bvdv) Associated With Cattle From  Mathura, Uttar Pradesh (India)

Puneet Varshney; S D Qureshi; A K Bhatia; H K  Pradhan; N Mishra; Sandeep Bhatia

doi:10.48165/

Authors

Puneet Varshney Department of Microbiology and Immunology, College of Veterinary Science and Animal Husbandry, Pandit Deen Dayal Upadhyay, Pashuchikitsa Vigyan Vishvavidhyalaya Evom Go Anusandhan Sansthan (DUVASU), Mathura, UP - 281 001, India
S D Qureshi Department of Microbiology and Immunology, College of Veterinary Science and Animal Husbandry, Pandit Deen Dayal Upadhyay, Pashuchikitsa Vigyan Vishvavidhyalaya Evom Go Anusandhan Sansthan (DUVASU), Mathura, UP - 281 001, India
A K Bhatia Department of Microbiology and Immunology, College of Veterinary Science and Animal Husbandry, Pandit Deen Dayal Upadhyay, Pashuchikitsa Vigyan Vishvavidhyalaya Evom Go Anusandhan Sansthan (DUVASU), Mathura, UP - 281 001, India
H K Pradhan High Security Animal Disease Laboratory, Indian Veterinary Research Institute, Anand Nagar, Bhopal, Madhya Pradesh - 462 021, India
N Mishra High Security Animal Disease Laboratory, Indian Veterinary Research Institute, Anand Nagar, Bhopal, Madhya Pradesh - 462 021, India
Sandeep Bhatia High Security Animal Disease Laboratory, Indian Veterinary Research Institute, Anand Nagar, Bhopal, Madhya Pradesh - 462 021, India

DOI:

https://doi.org/10.48165/

Keywords:

BVDV, RT-PCR, PBL’s, ELISA

Abstract

Bovine viral diarrhea (BVD) infection causing great reproductive and productive losses is an immunosuppression seen in all age groups of ruminants world-wide. In present study out of 78 peripheral blood leucocyte (PBLs) samples 4 samples were positive for BVDV antigen by pestivirus antigen-capture ELISA, while none of the faecal samples was positive for BVDV antigen by AGID and RT-PCR. The use of RT-PCR on all PBLs samples was restricted to the above 4 positive samples which yielded a 288 bp amplicon, while rest were found negative. The BVDV positive samples were confirmed by restriction endonuclease analysis. The study revealed the presence of BVDV in Mathura region of Uttar Pradesh.

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References

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