Molecular And Antibody Based Diagnosis Of Bovine Viral Diarrhea Virus (Bvdv) Associated With Cattle From Mathura, Uttar Pradesh (India)

Authors

  • Puneet Varshney Department of Microbiology and Immunology, College of Veterinary Science and Animal Husbandry, Pandit Deen Dayal Upadhyay, Pashuchikitsa Vigyan Vishvavidhyalaya Evom Go Anusandhan Sansthan (DUVASU), Mathura, UP - 281 001, India
  • S D Qureshi Department of Microbiology and Immunology, College of Veterinary Science and Animal Husbandry, Pandit Deen Dayal Upadhyay, Pashuchikitsa Vigyan Vishvavidhyalaya Evom Go Anusandhan Sansthan (DUVASU), Mathura, UP - 281 001, India
  • A K Bhatia Department of Microbiology and Immunology, College of Veterinary Science and Animal Husbandry, Pandit Deen Dayal Upadhyay, Pashuchikitsa Vigyan Vishvavidhyalaya Evom Go Anusandhan Sansthan (DUVASU), Mathura, UP - 281 001, India
  • H K Pradhan High Security Animal Disease Laboratory, Indian Veterinary Research Institute, Anand Nagar, Bhopal, Madhya Pradesh - 462 021, India
  • N Mishra High Security Animal Disease Laboratory, Indian Veterinary Research Institute, Anand Nagar, Bhopal, Madhya Pradesh - 462 021, India
  • Sandeep Bhatia High Security Animal Disease Laboratory, Indian Veterinary Research Institute, Anand Nagar, Bhopal, Madhya Pradesh - 462 021, India

DOI:

https://doi.org/10.48165/

Keywords:

BVDV, RT-PCR, PBL’s, ELISA

Abstract

Bovine viral diarrhea (BVD) infection causing great  reproductive and productive losses is an immunosuppression  seen in all age groups of ruminants world-wide. In present study  out of 78 peripheral blood leucocyte (PBLs) samples 4 samples  were positive for BVDV antigen by pestivirus antigen-capture  ELISA, while none of the faecal samples was positive for BVDV  antigen by AGID and RT-PCR. The use of RT-PCR on all  PBLs samples was restricted to the above 4 positive samples  which yielded a 288 bp amplicon, while rest were found  negative. The BVDV positive samples were confirmed by restriction endonuclease analysis. The study revealed the presence of BVDV in Mathura region of Uttar Pradesh. 

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References

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Published

2012-10-03

Issue

Vol. 14 No. 2 (2012): Applied Biological Research (Jul) 2012

Section

Short Communications

How to Cite

Molecular And Antibody Based Diagnosis Of Bovine Viral Diarrhea Virus (Bvdv) Associated With Cattle From Mathura, Uttar Pradesh (India) . (2012). Applied Biological Research, 14(2), 215–219. https://doi.org/10.48165/