Sterilization Strategies For Aseptic In Vitro Establishment  And Survival Of Shoot Tip Explants In Gerbera Jamesonii Bolus

Imtiyaz; T  Nazki; Z A Rather; Nelofar; G Hussain; M A Mir

doi:10.48165/

Authors

Imtiyaz S.K. University of Agricultural Sciences and Technology of Kashmir, Shalimar, Srinagar, Jammu & Kashmir - 191 121 India
T Nazki S.K. University of Agricultural Sciences and Technology of Kashmir, Shalimar, Srinagar, Jammu & Kashmir - 191 121 India
Z A Rather S.K. University of Agricultural Sciences and Technology of Kashmir, Shalimar, Srinagar, Jammu & Kashmir - 191 121 India
Nelofar S.K. University of Agricultural Sciences and Technology of Kashmir, Shalimar, Srinagar, Jammu & Kashmir - 191 121 India
G Hussain S.K. University of Agricultural Sciences and Technology of Kashmir, Shalimar, Srinagar, Jammu & Kashmir - 191 121 India
M A Mir Division of Floriculture, Medicinal and Aromatic Plants, Division of Pomology,Shalimar, Srinagar, Jammu & Kashmir - 191 121 India

DOI:

https://doi.org/10.48165/

Keywords:

Asepsis, gerbera, in vitro, sterilization, survival

Abstract

The study involved two experiments aimed at comparing post-sterilization in vitro asepsis and subsequent survival of gerbera shoot tip explants sourced from either field-grown or pot-raised plants under pro-phylactic fungicide cover and shoot tip explants with or without leaves. Six sterilant-combinations used were S1 [carbendazim 50 WP @ 200 ppm for 30 min. + 0.1% mercuric chloride (HgCl2) for 7.5 min.]; S2 (carbendazim 200 ppm for 30 min. + 0.1% HgCl2 for 10 min.); S3 (0.05% Megapin + S1); S4 (0.05% Megapin + S2); S5 (S3 + 70% ethyl alcohol dip for 10 sec.); S6 (S4 + 70% ethyl alcohol dip for 10 sec.). Post-sterilization culture asepsis in shoot tips sourced from fungicide-treated plants and shoot tips without appended leaves was significantly high as compared to those sourced from field-grown plants or shoot tips with leaves intact. Amongst the sterilants,treatment-combinations of explant dip for 10 sec. in 70% ethyl alcohol proved most effective in reducing infection in vitro. Survival of shoot tips with leaves intact was higher in comparison to those without leaves. Treatments involving 70% ethyl alcohol dip of explants improved post-sterilization survival significantly as compared to the treatment combinations wherein ethyl alcohol was not used.

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References

Aswath, C. and Choudhary, M.L. 2002. Mass propagation of gerbera (Gerbera jamesonii) through shoot culture. Indian Journal of Horticulture, 59: 95-99.

Aswath, C. and Choudhary, M.L. 2001. Effect of cytokinins on proliferation of multiple shoots in gerbera (Gerbera jamesonii). Indian Journal of Horticulture, 58: 383-386.

Aswath, C. and Nazneen, S. 2004. An improved method for in vitro propagation of gerbera. Journal of Ornamental Horticulture New Series, 7: 141-146.

Sterilization strategies for in vitro establishment and survival of gerbera 197

Jerzy, M. and Lubomski, M. 1991. Adventitious shoot formation on ex vitro derived leaf explants of Gerbera jamesonii. Scientia Horticulturae, 47: 115-124.

Jothi, L.J., Balakrishnamoorthy, G., Vijayakumar, M. and Murugesan, R. 2003. Comparative evaluation of in vitro progenies of gerbera. South Indian Horticulture, 51: 70-75.

Kanwar, J.K. and Kumar, S. 2008. In vitro propagation of gerbera - A review. Horticultural Science, 35: 35-44.

Nafees, A., Khan, A.R. and Bhatti, I.A. 2009. Tissue culture of gerbera. Pakistan Journal of Botany, 41: 7-10

Parthasarathy, B., Satayahari, D., Nilanjana, D. and Bhattacharrya, B.C. 1990. Rapid mass propagation of Chrysanthemum morifolium by callus derived from stem and leaf explants. Plant Cell Reports, 9: 439-442.

Posada, M., Ballesteros, N., Obando, W. and Angarita, A. 1999. Micropropagation of gerberas from floral buds. Acta Horticulturae, 482: 329-332.

Roalte, D van. 1978. Gerbera breeding and propagation. Deutscher Gartenbau, 32: 1754-1755. Ruffoni, B., Damiano, C; Silvano, G; Bregliano, R. 1987. The sterilization and micropropagation of Gerbera jamesonii hybrida. Annali dell'Istituto-Sperimentale per la Floricoltura (Italy), 18: 21-42.