Impact Of Biofertilizers On Growth, Nutrient Uptake, Yield,  Metabolism And Rhizosphere Enzyme Activities Of Pomegranate  (Punica Granatum L.) Cv. ‘kandhari Kabuli’

Muzaffar Mir; G I Hassan; K Sheikh; Sanjeev Kumar Sharma

doi:10.48165/

Authors

Muzaffar Mir Department of Fruit Science, Dr. Y.S. Parmar University of Horticulture and Forestry, Nauni, Solan, Himachal Pradesh -173 230 (India)
G I Hassan Department of Fruit Science, Dr. Y.S. Parmar University of Horticulture and Forestry, Nauni, Solan, Himachal Pradesh -173 230 (India)
K Sheikh Department of Entomology, CS K Himachal Pradesh Krishi Vishwavidyalaya, Palampur, Himachal Pradesh -176 062 (India)
Sanjeev Kumar Sharma Department of Microbiology, Dr. Y.S. Parmar University of Horticulture and Forestry, Nauni, Solan, Himachal Pradesh -173 230 (India)

DOI:

https://doi.org/10.48165/

Keywords:

Azotobacter, mycorrhizal fungi, pomegranate, soil enzymes, PSB, yield

Abstract

Nursery and field experiments were carried out to assess the effectiveness of N2-fixing bacteria, phosphate solubilizing bacteria and arbuscular mycorrhizal (AM) fungi, alone or in combination, on the growth and biomass production of pomegranate (Punica granatum). The study revealed significant improvement in nursery seedlings growth by biofertilizers use. Combined treatment of Azotobacter chroococcum + Glomus mosseae proved most effective in enhancing the rhizosphere microbial activity and concentration of various metabolites and nutrients in pomegranate in nursery experiments as well as helped in better plant establishment under field conditions. A significant improvement in plant height and fruit yield was observed in pomegranate plants under field conditions. Biofertilizer application also improved plant nutrient uptake and rhizosphere microbial activities. Results indicate that biofertilizer technology may be adopted for the establishment and development of horticultural plant species in rainfed agro-ecosystem.

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References

Arnon, D.I. 1949. Copper enzymes in isolated chloroplast: Polyphenol oxidase in Beta vulgaris. Plant Physiology, 24: 1-15.

Aseri, G.K., Neelam Jain, Jitendra Panwar, Rao, A.V. and Meghwal, P.R. 2008. Biofertilizers improve plant growth, fruit yield, nutrition, metabolism and rhizosphere enzyme activities of pomegranate (Punica granatum L.) in Indian Thar desert. Scientia Horticulture, 117: 130-135.

Ceccanti, B., Pezzarossa, B., Gallardo-Lancho, F.J. and Masciandaro, G. 1994. Bio-tests as markers of soil utilization and fertility. Geomicrobiol Journal, 11: 309-316.

Day, J.M. and Dobereiner, J. 1976. Physiological aspects of N2-fixation by a Spirillum from Digitaria roots. Soil Biology and Biochemistry, 8: 45-50.

Muzaffar Mir et al.

Ephraim, P.L. and Robert, A.N. 2007. Punica granatum (pomegranate) and its potential for prevention and treatment of inflammation and cancer. Journal of Ethanopharmacy, 109: 177-206. Gaur, A. and Adholeya, A. 1994. Estimation of VAMF spores in soil: A modified method. Mycorrhiza News, 6: 10-11.

Gerdemann, J.W. and Nicolson, T.H. 1963. Spores of mycorrhizal Endogone species extracted from soil by wet sieving and decanting, Transactions of British Mycological Society, 46: 235-244. Jackson, M.L. 1967. Soil Chemical Analysis. Prentice-Hall of India Pvt. Ltd., New Delhi, India. Jeeva, S., Kulasekaran, M., Shanmugavelu, K.G. and Oblisami, G. 1988. Effect of Azospirillum on growth and development of banana cv. Poovan (AAB). South Indian Horticulture, 36: 1-4. Jensen, H.L. 1954. The Azotobacteriace. Bacteria Review, 18: 195-214.

Karlidaga, H., Esitken A., Turanc, M and Sahind, F. 2007. Effects of root inoculation of plant growth promoting rhizobacteria (PGPR) on yield, growth and nutrient element contents of leaves of apple. Scientia Horticulture, 114: 16-20.

Khanezadeh, S., Hamel, C., Kianmehr, H., Buszard, D. and Smith, D.L.1995. Effect of three arbuscular mycorrhizal fungus species and phosphorus on productivity and vegetative growth of three strawberry cultivars. Journal of Plant Nutrition, 18: 1073-1079.

Kohler, J., Caravaca, F., Carrasco, L. and Rolden, A. 2007. Interactions between a plant growth promoting rhizobacterium, an AM fungus and phosphate-solublizing fungus in the rhizosphere of Lactuca sativa. Applied Soil Ecology, 35: 480-487.

Luis, J., Martinez, M., Lucia, S.U., Beatriz, E.B., Jose, A. and Sanchez, A. 2003. Indole 3-butyric acid (IBA) production in culture medium by wild strain Azospirillum brasilense. FEMS Microbiology Letters, 228: 167-173.

Mahadhevan, A., Kui, J. and George, E. 1965. Biochemistry of resistance in cucumber against Cladosporium cucumerinum in presence of pectinase inhibitor in white clover. Plant and Soil. 136: 49-57.

Mathur, N. and Vyas, A. 2000. Influence of arbuscular mycorrhizae on biomass production, nutrient uptake and physiological changes in Ziziphus mauritiana Lam. under water stress. Journal of Arid Environment, 45: 191-195.

Michel, D.S., Melanie, E.R.N., Gerdi, W., Jennifer, J.D., Mailine, H.C., Ruth, M., Caren, J., Raisin, R.N. and Dean, O. 2005. Effect of pomegranate juice consumption on mycocardial perfusion in patient with coronary heart disease. American Journal of Cardiology. 96: 810-814.

Panwar, J. and Tarafdar, J.C. 2006. Distribution of three endangered medicinal plant species and their colonization with arbuscular mycorrhizal fungi. Journal of Arid Environment, 65: 337-350. Patel, V.B., Singh, S.K., Asery Ram, Lata Nain, Singh, A.K. and Laxman Singh. 2009. Microbial and inorganic fertilizers application influenced vegetative growth, yield, leaf nutrient status and soil microbial biomass in sweet orange cv. Mosambi. Indian Journal of Horticulture, 66: 163-68. Rao, A.V. and Venkateswarlu, B. 1982. Nitrogen fixation by Azospirillum isolated from tropical grasses native to Indian desert. Indian Journal of Experimental Biology, 20: 316-318.

Read, D.J., Kouckeki, N.K. and Hodgson, J. 1976. Vesicular arbuscular mycorrhizae in natural vegetative system: The occurrence of infection. New Phytology, 77: 641-653.

Saxena, A.K. and Tilak, K.V.B.R. 1994. Interaction among beneficial soil microorganisms. Indian Journal of Microbiology, 34: 91-103.

Schenck, N.C. and Perez, Y. 1990. Manual for the Identification of VA Mycorrhizal Fungi (3rd edn.), Synergistic Publications, Gainesville, Florida, USA.

Schnurer, J. and Rosswall, T. 1982. Fluorescein di-acetate hydrolysis as a measure of total microbial activity in soil and litter. Applied Environmental Microbiology, 43: 1256-1261. Sharma, S.D., Kumar, P., Singh, S.K. and Patel, V.B. 2009. Indigenous AM fungi and Azotobacter, and their screening from citrus seedlings at different levels of inorganic fertilizers application. Indian Journal of Horticulture, 66: 183-189.

Biofertilizer use in pomegranate 175

Sharma, S.D., Sharma, N., Sharma, C.L., Sood, R. and Singh, R.P. 2008. Studies on correlation between endomycorrhizal and Azotobacter population with growth, yield and soil nutrient status of apple (Malus domestica Borkh) orchards in Himachal Pradesh. Acta Horticulturae, 696: 283- 287.

Singh, C. and Sharma, B.B. 1993. Leaf nutrient composition of sweet orange as affected by combined use of bio and chemical fertilizers. South Indian Horticulture, 41: 131-134.

Singh, A. and Singh, S.P. 2004. Response of banana (Musa sp.) to vesicular arbuscular mycorrhizae and varied levels of inorganic fertilizers. Indian Journal of Horticulture, 61: 109-113. Skujins, J. 1973. Dehydrogenase: An indicator of biological activities in arid soils. Bulletin Ecological Research Communication, 17: 235-241.

Tabatabai, M.A. 1982. Soil enzymes. pp. 903–947. In: Methods of Soil Analysis. Part 2. Chemical and Microbiological Properties (Eds. A.L. Page, R.H. Miller and D.R. Keeney). American Society of Agronomy, USA.

Tabatabai, M.A. and Bremner, J.M. 1969. Use of ρ-nitrophenyl phosphate for assay of soil phosphatase activity. Soil Biology and Biochemistry, 1: 301-307.

Tarafdar, J.C. and Gharu, A. 2006. Mobilization of organic and poorly soluble phosphates by Chaetomium globosum. Applied Soil Ecology, 32: 273-283.