Studies On In Vitro Morphogenetic Potential Of Nodal  Segments Of Malaxis Acuminata D. Don

T Arenmongla; Chitta Ranjan Deb

doi:10.48165/

Authors

T Arenmongla Department of Botany, Nagaland University, Lumami 798 627, Nagaland, India
Chitta Ranjan Deb Department of Botany, Nagaland University, Lumami 798 627, Nagaland, India

DOI:

https://doi.org/10.48165/

Keywords:

Malaxis acuminata, medicinal orchid, micro propagation, nodal explants

Abstract

Malaxis acuminata is a therapeutically important terrestrial orchid. Due to various anthropogenic activities and over-collection for medicinal purposes the species is under threat in its natural habitat. A successful attempt was made to propagate this species using nodal segments from axenic source as alternative explants. Invocation of meristematic loci from the cultured nodal segments initiated within three weeks of culture on MS medium fortified with sucrose (3%, w/v), NAA and BA (3 µM each in combination). Under this condition as many as 18 shoot buds developed. The resultant shoot buds converted into plantlets with well developed roots on MS medium supplemented with sucrose (3%), NAA and BA (3 µM each in combination) where average plant height and number of leaves, shoots and roots were 2.4 cm, 4.5, 18.0 and 4.0, respectively. The hardened regenerates were transferred to community potting mix prepared by mixing charcoal pieces, chopped forest litter, coconut husk, sand and black soil in equal proportion. About 75% of transplants survived after 2 months of transfer.

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References

Arditti, J. and Ernst, R. 1993. Micropropagation of Orchids. John Wiley & Sons Inc., New York, USA. Bhadra, S.K. and Hossain, M.M. 2004. Induction of embryogenesis and direct organogenesis in Micropera pallida Lindl., an epiphytic orchid of Bangladesh. Journal of Orchid Society of India, 18: 5-9.

T. Arenmongla and Chitta Ranjan Deb

Chen, T.Y., Chen, J.T. and Chang, W.C. 2004. Plant regeneration through direct shoot bud formation from leaf cultures of Paphiopedilum orchids. Plant Cell Tissue and Organ Culture, 76: 11-15. Deb, C.R. and Imchen, T. 2010. An efficient in vitro hardening technique of tissue culture raised plants. Biotechnology, 9: 79-83.

Deb, C.R. and Pongener, A. 2012. Development of a cost effective in vitro regenerative protocol of Cymbidium aloifolium (L.) Sw. using nodal segments as an explants source. International Journal of Chemical and Biological Sciences, 1: 77-84.

Deb, C.R. and Sungkumlong. 2010. Regenerative competence of foliar explants of Coelogyne suaveolens and Taenia latifolia: two threatened orchids of North-East India. Applied Biological Research, 12: 1-9.

Deb, C.R. and Temjensangba. 2005. In vitro regenerative competence of Cleisostoma racemiferum (Orchidaceae) aerial roots. Journal of Plant Biochemistry and Biotechnology, 14: 201-205. Deb, C.R. and Temjensangba. 2006a. In vitro propagation of threatened terrestrial orchid, Malaxis

khasiana Soland ex. Swartz through immature seed culture. Indian Journal of Experimental Biology, 44: 762-766.

Deb, C.R. and Temjensangba. 2006b. On the regeneration potential of Arachnis labrosa (Lindl. Ex. Paxt.) Reichb. root segments: A study in vitro. Phytomorphology, 56: 79-83.

Deb, C.R. and Temjensangba. 2007a. Direct regeneration of shoot buds in Arachnis labrosa foliar explants. Journal of Orchid Society of India, 21: 7-9.

Deb, C.R. and Temjensangba. 2007b. Rapid mass multiplication of Cleisostoma racemiferum (Lindl.) Garay: An endangered orchid. Journal of Plant Biology, 34: 99-105.

George, E.F. and Sherrington, P.D. 1984. Plant Propagation by Tissue Culture: Handbook and Directory of Commercial Laboratories. Exegetics Ltd., Hants, Eversley, UK.

Kaur, S. and Vij, S.P. 2000. Regeneration potential of Saccolabium papillosum leaf segments. Journal of Orchid Society of India, 14: 67-73.

Kosir, P., Skof, S. and Luthar, Z. 2004. Direct shoot regeneration from nodes of Phalaenopsis orchids. Acta Agricultural Slovenica, 83: 233-242.

Kumaria, S. and Tandon, P. 1994. Clonal propagation and establishment of plantlets of Dendrobium fimbriatum var. oculatum Hook.f. pp. 218-231. In: Advances in Plant Tissue Culture in India (ed. P. Tandon). Pragati Prakashan, Meerut. India.

Lavanya, M., Venkateshwarlu, B. and Devi, B.P. 2009. Acclimatization of neem microshoots adaptable to semi-sterile conditions. Indian Journal of Biotechnology, 8: 218-222.

Mitra, G.C., Prasad, R.N. and Roy Chowdhury, A. 1976. Inorganic salt and differentiation of protocorms in seed callus of an orchid and correlated changes in its free amino acid content. Indian Journal of Experimental Biology, 14: 350-351.

Murashige, T. and Skoog, F. 1962. A revised medium for rapid growth and bioassays with tobacco tissue culture. Physiologia Plantarum, 15: 473-497.

Paul, T.L. 1999. Tissue culture techniques in in vitro plant conservation, pp. 2-62. In: Plant Conservation Biotechnology (ed. E. E. Benson). Taylor & Francis Ltd, London, UK. Pongener, A. and Deb, C.R. 2009. Asymbiotic culture of immature embryos, mass multiplication of Cymbidium iridioides Don and the role of different factors. International Journal Pharma and Bio Sciences, 1: 1-14.

Pongener, A. and Deb, C.R. 2011a. In vitro mass multiplication of Cymbidium aloifolium using immature embryos as explant. Journal of Plant Biochemistry and Biotechnology, 20: 90-95. Pongener, A. and Deb, C.R. 2011b. In vitro regeneration of plantlets of Cymbidium irridioides Don

using nodal segments as explants. International Journal of Applied Biotechnology and Biochemistry, 1: 389-400.

Roy, J. and Banerjee, N. 2003. Induction of callus and plant regeneration from shoot-tip explants of Dendrobium fimbriatum Lindl. Var. oculatum HK. F. Scientia Horticulturae, 97: 333-340.

In vitro morphogenetic potential of nodal segments of Malaxis acuminata 163

Seeni, S. and Latha, P.G. 1992. Foliar regeneration of endangered red Vanda Renanthera imschootiana Rolfe (Orchidaceae). Plant Cell Tissue and Organ Culture, 29: 167-172.

Temjensangba and Deb, C.R. 2005. Regeneration and mass multiplication of Arachnis labrosa (Lindl. ex Paxt.) Reichb: A rare and threatened orchid. Current Science, 88: 1966-1969. Temjensangba and Deb, C.R. 2006. Effect of different factors on non-symbiotic seed germination, formation of protocorm-like bodies and plantlet morphology of Cleisostoma racemeferum (Lindl.) Garay. Indian Journal of Biotechnology, 5: 223-228.

Tisserat, B. and Jones, D. 1999. Clonal propagation of orchids, pp. 127-134. In: Plant Cell Culture Protocols: Methods in Molecular Biology, III (Ed. R.D. Hall), Humana Press Inc., Totowa, New Jersy, USA.

Vij, S.P. and Aggarwal, S. 2003. Regenerative competence of foliar explants: Vanda Coerulea Griff. Journal of Orchid Society of India, 17: 73-78.

Yapo, E.S., Kouakou, T.H., Kone, M., Kouadio, J.Y., Kouame, P. and Merillon, J.M. 2011. Regeneration of pineapple (Ananas comosus L.) plant through somatic embryogenesis. Journal of Plant Biochemistry and Biotechnology, 20: 196-204.

Yarra, R., Aileni, M., Vemunoori, A.K., Kokkirala, V.R., Umate, P. and Abbagani, S. 2010. Direct shoot regeneration from mature leaf explants of Sphaeranthus indicus L., A multipurpose medicinal plant. Journal of Phytology, 2(5): 5-11.