Somatotropin Effective Antisera Production In Forty Days

Authors

  • Sumbul Khalid Department of Bioinformatics and Biotechnology, Women Campus, International Islamic University, Islamabad, Pakistan
  • Azra Khanum Department of Biochemistry, Pir Mehr Ali Shah Arid Agriculture University, Murree Road, Rawalpindi, Pakistan

DOI:

https://doi.org/10.48165/

Keywords:

Affinity column, polyclonal antisera, protein purification, somatotropin

Abstract

Polyclonal antisera were raised against bovine somatotropin (ST) in rabbits  and high titer achieved in less than 40 days after primary immunization.  Protocol was developed with slight modification in method of Hu (2002) for  antisera production and booster injection given on 28th and 37th day. The  serum for antibody titer was collected on 35th and 42nd day. The antisera  procured were effective in Western blotting and for purification of ST using  affinity column chromatography. 

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References

Bradford, M.M. 1976. A dye binding assay for protein. Analytical Biochemistry, 72: 248-254. CCAC. 2002. Guidelines on Antibody Production. Canadian Council on Animal Care, Ottawa, Canada (http://www.ccac.ca/Documents/Standards/Guidelines/Antibody_production.pdf). Harkins, K.R. 2001. Basic methods in antibody purification and characterization. pp.142-168. In: Basic Methods in Antibody Production and Characterization (eds. G.C. Howard and D.R. Bethell), CRC Press, Florida, USA.

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Khalid, S., Mehmood, S.F., Shahzad, M.I., Bashir, Q., Saba, S.Z., Mustansar, Z., Fatima, A. and Khanum, A. 2008. Cloning, expression and modeling studies of somatotropin cDNA of local buffalo breed-Nili Ravi. Journal of Chemical Society of Pakistan, 30: 712-725.

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Leenaars, M. and Hendriksen, F.M. 2005. Critical steps in the production of polyclonal and monoclonal antibodies: Evaluation and recommendations. Institute of Laboratory Animal Research Journal, 46: 269-279.

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Published

2013-05-15

Issue

Vol. 15 No. 1 (2013): Applied Biological Research (Jan) 2013

Section

Short Communications

How to Cite

Somatotropin Effective Antisera Production In Forty Days . (2013). Applied Biological Research, 15(1), 70–72. https://doi.org/10.48165/
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