Antimicrobial And Phytochemical Screening Of Rhizome  Extracts Of Some Native Medicinal Plant Of Himachal  Pradesh (India)

Amit Pratush; Sarita Dogra; Anupama Gupta

doi:10.48165/

Authors

Amit Pratush Department of Biotechnology, Shoolini Institute of Life Sciences and Business Management (SILB), The Mall, Solan, Himachal Pradesh (India)
Sarita Dogra Department of Biotechnology, Shoolini Institute of Life Sciences and Business Management (SILB), The Mall, Solan, Himachal Pradesh (India)
Anupama Gupta Department of Microbiology, Dr. Y.S. Parmar University of Horticulture and Forestry, Nauni, Solan, Himachal Pradesh (India)

DOI:

https://doi.org/10.48165/

Keywords:

Antimicrobial activity aqueous extract, ethanolic extract, phytochemical, pathogens, rhizome

Abstract

The ethanolic and aqueous extracts of rhizomes of five medicinal plants (Agave sisaliana, Euphorbia royleana, Zantedeschia aetthiopica, Stephania glabra and Mirabilis jalapa), collected from Solan district of Himachal Pradesh (India), were assayed for antimicrobial activity against eight bacteria including some human pathogens. The ethanolic and aqueous extracts of these plants were less effective against Escherichia coli, Bacillus subtilis and Staphylococcus aureus whereas M. jalapa extract was ineffective against Shigella sp. The qualitative phytochemical analysis confirmed the presence of various phytochemicals like tannins, alkaloids, saponins, steroids, phenols and glycosides in the rhizome extracts of these plants. The plant extracts tested possessed antimicrobial potential. Further, the presence of phytochemicals such as tannins, alkaloids, saponins, etc. in the rhizome extracts of these plants suggest their anticancer potential.

Downloads

Download data is not yet available.

References

Adebayo, A.O., Adewumi, C.O. and Essien, E.E. 1983. Anti-infective agent of higher plants. pp. 152- 158. In: International Symposium of Medicinal Plants (5th edn.). University of Ife, Ife, Nigeria. Asif, M. 2012. A Review on phytochemical and ethnopharmacological activities of Curculigo orchioides. Mahidol University Journal of Pharmaceutical Sciences, 39: 1-10. Auta, K.I., Galadima, A.A., Bassey, J.U., Olowoniyi, O.D., Moses, O.O. and Yako, A.O. 2011. Antimicrobial properties of the ethanolic extracts of Zingiber officinale (ginger) on Escherichia coli and Pseudomonas aeruginosa. Research Journal of Biological Sciences, 6: 37-39. Bauer, A.W., Kirby, W.M.M., Sherries, J.C. and Truck, M. 1966. Antibiotic susceptibility testing by standard single disc diffusion method. American Journal of Clinical Pathology, 45: 426-493. Belguith, H., Kthiri. F., Chati, A., Sofah, A.A., Hamida, J.B. and Landoulsi, A. 2010. Study of the effect of aqueous garlic extract (Allium sativum) on some Salmonella serovars isolates. Emirate Journals of Food and Agriculture, 22: 189-206.

Gull, I., Saeed, M., Shaukat, H., Aslam, S.M., Samra, Z.Q. and Athar A.M. 2012. Inhibitory effect of Allium sativum and Zingiber officinale extracts on clinically important drug resistant pathogenic bacteria. Annals of Clinical Microbiology and Antimicrobiology, 11: 8. [doi: 10.1186/1476- 0711-11-18].

Gur, S., Turgut-Balik, D. and Gur, N. 2006. Antimicrobial activities and some fatty acids of turmeric, ginger root and linseed used in the treatment of infectious diseases. World journal of Agricultural Sciences, 2: 439-442.

Harborne, J.B. 1998. Phytochemical Methods. Chapman and Hall, London, UK. Iwu, M. W., Duncan, A.R. and Okunji, C.O. 1999. New antimicrobials of plant origin. pp. 457-462. In: Perspectives on New Crops and New Uses (ed. J. Janick), Alexandria, ASHS Press, Australia.

Kaur, G.J. and Arora, D.S. 2009. Antibacterial and phytochemical screening of Anethum graveolens, Foeniculum vulgare and Trachyspermum ammi. BMC Complementary and Alternative Medicine, 9: 30 [doi: 10.1186/1472-6882-9-30.]

Kennedy D.O. and Wightman E. L. 2011. Herbal extracts and phytochemicals: Plant secondary metabolites and the enhancement of human brain function. Advances in Nutrition, 2: 32-50. Muthumani, P., Devi, P., Meera, R., Kameshwari, B. and Eswarapriya, B. 2009. In vitro antimicrobial

activity of various extracts of Mirabilis jalapa leaves. The International Journal of Microbiology, 7: 120-124.

Oskay, M., Oskay, D. and Kalyoncu, F. 2009. Activity of some plant extracts against multi-drug resistant human pathogens. Iranian Journal of Pharmacological Research, 8: 293-300.

Antimicrobial and phytochemical screening of some rhizome extracts 153

Pavia, P.M.G., Gomes, F.S., Napoleaeo, T.H., Sae, R.A., Correia, M.T.S. and Coelho L.C.B.B. 2010. Antimicrobial activity of secondary metabolites and lectins from plants. Applied Microbiology and Microbial Biotechnology. 396-406.

Poeloengan, M. 2011. The effect of red ginger (Zingiber officinale Roscoe) extract on the growth of mastitis causing bacterial isolates. African Journal of Microbiology Research, 5: 382-389. Ravi Sankar, N. and Sreeramulu, A. 2009. In vitro efficacy of nineteen medicinal plant extracts on

Uncinula tectonae, the powdery mildew of teak. Journal of Biomedicine, 3: 137-141. Ríos, J.L. and Recio, M.C. 2005. Medicinal plants and antimicrobial activity. Journal of Ethno pharmacology, 100: 80-84.

Sameera R, Samarakoon, I.T., Prasanna B.G., Dilip De Silva and Kamani, H.T. 2010. A comparison of the cytotoxic potential of standardized aqueous and ethanolic extracts of a polyherbal mixture comprised of Nigella sativa (seeds), Hemidesmus indicus (roots) and Smilax glabra (rhizome). Pharmacognosy Research, 2: 335-342.

Sofowora, A. 1993. Medicinal Plants and Traditional Medicine in Africa. Spectrum Books limited, Ibadan, Nigeria.

Yusha’u, M., Garba, L. and Shamsuddeen, U. 2008. In vitro inhibitory activity of garlic and ginger extracts on some respiratory tract isolates of Gram-negative organisms. International Journal of Biomedical Health Sciences, 4: 57-60.