Optimization Of Cultural Conditions For Cellulase-Free  Xylanase Production By Mutant Strain Of Alkalophilic  Cellulosimicrobium Sp. Ckmx1 In Submerged Fermentation

Shiwani Guleria; Abhishek Walia; Anjali Chauhan; C K  Shirkot

doi:10.48165/

Authors

Shiwani Guleria Department of Basic Sciences (Microbiology Section), Dr. Y. S. Parmar University of Horticulture and Forestry, Nauni, Solan - 173 230, Himachal Pradesh (India)
Abhishek Walia Department of Basic Sciences (Microbiology Section), Dr. Y. S. Parmar University of Horticulture and Forestry, Nauni, Solan - 173 230, Himachal Pradesh (India)
Anjali Chauhan Department of Basic Sciences (Microbiology Section), Dr. Y. S. Parmar University of Horticulture and Forestry, Nauni, Solan - 173 230, Himachal Pradesh (India)
C K Shirkot Department of Basic Sciences (Microbiology Section), Dr. Y. S. Parmar University of Horticulture and Forestry, Nauni, Solan - 173 230, Himachal Pradesh (India)

DOI:

https://doi.org/10.48165/

Keywords:

Alkalophilic bacterium, Cellulosimicrobium, mushroom compost, mutation, xylanase

Abstract

This paper reports optimization of cultural conditions for xylanase production bymutantstrains of Cellulosimicrobiumsp. CKMX1 in submerged fermentation. Alkalophilic Cellulosimicrobium sp. CKMX1, capable of producing xylanase, was isolated from mushroom compost. Xylanase production by this strain was enhanced by ethyl methanesulfonate (5-70 mg mL-1) mutation and by optimizing the process parameters in submerged fermentation. Mutant strain E5 with hyper xylanase production was obtained after treating wild strain with ethyl methanesulfonate on the basis of xylanase activity index and xylanase activity in liquid medium. Cultural conditions were optimized for parent and mutant strain and incubation period of 72 h, incubation temperature of 35+1⁰ C, pH of 8.0; 1% inoculum size: and xylan as carbon source yielded high xylanase. Mutant E5 was 22.99% more efficient than parent strain. Results indicated the potential of alkalophilic Cellulosimicrobium sp. CKMX1 and its hyper xylanase producing mutant strain E5 to be promising for pulp and paper industry.

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