Micropropagation Studies In Almond (Prunus Dulcis Mill.) Cv. ‘shalimar’

M A Mir; K M  Bhat; Z A Rather; A H Pandit; F A Peer; G  Hussain

doi:10.48165/

Authors

M A Mir Division of Fruit Science,Medicinal and Aromatic Plants, S.K. University of Agricultural Sciences and Technology of Kashmir, Shalimar, Srinagar - 191 121, Jammu & Kashmir (India)
K M Bhat Division of Fruit Science, Medicinal and Aromatic Plants, S.K. University of Agricultural Sciences and Technology of Kashmir, Shalimar, Srinagar - 191 121, Jammu & Kashmir (India)
Z A Rather Division of Floriculture, Medicinal and Aromatic Plants, S.K. University of Agricultural Sciences and Technology of Kashmir, Shalimar, Srinagar - 191 121, Jammu & Kashmir (India)
A H Pandit Division of Fruit Science, Medicinal and Aromatic Plants, S.K. University of Agricultural Sciences and Technology of Kashmir, Shalimar, Srinagar - 191 121, Jammu & Kashmir (India)
F A Peer Division of Fruit Science, Medicinal and Aromatic Plants, S.K. University of Agricultural Sciences and Technology of Kashmir, Shalimar, Srinagar - 191 121, Jammu & Kashmir (India)
G Hussain Division of Fruit Science, Medicinal and Aromatic Plants, S.K. University of Agricultural Sciences and Technology of Kashmir, Shalimar, Srinagar - 191 121, Jammu & Kashmir (India)

DOI:

https://doi.org/10.48165/

Keywords:

Almond, in vitro propagation, micro-propagation, proliferation, shoot-tip culture

Abstract

Surface-sterilized forced shoots tips were inoculated on different basal media supplemented with various combinations of growth regulators. Quality and quantity of growth regulators and nutrient media were found crucial for culture establishment. Optimum culture establishment was achieved on ½MS medium containing benzylamino purine (BAP) + indole butyric acid (IBA) [0.50 + 0.1 mg L-1] where 63.33% morphogenetic response was observed. Callusing at the base of initiating cultures was minimum with BAP + IBA (0.25 + 0.01 mg L-1). Micro-shoots from the established cultures were sub-cultured on MS media supplemented with BAP and NAA (naphthalene acetic acid) alone or in combination for axillary shoot proliferation. Maximum proliferated cultures (80%) with high number of shoots explant-1(13.7) and proliferation grade (3.9) was obtained with BAP (0.40 mg L-1) + NAA (0.01 mg L-1). BAP proved superior to NAA during axillary shoot proliferation. Micro-shoots (10-15 mm) from proliferated cultures were subcultured in root induction medium (MS medium supplemented with IBA) and incubated under darkness for 10 days at 24±1oC and then transferred to root development medium (hormone-free MS medium) and incubated under normal culture room conditions. The rooting of micro-shoots, root length and number of roots shoot-1 were highest in treatment IBA (1.0 mg L-1).

Downloads

Download data is not yet available.

References

Anonymous. 2012. Horticulture Economic Survey. Department of Horticulture, Jammu and Kashmir Government, Srinagar (J&K) [http://www.jandkplanning.com/images/Economic_Survey/17- horticulture.pdf].

Aghaye, R.N.M. 2012. Micropropagation of GF-677 rootstock. Journal of Agricultural Science, 4(5): 131-138

Ainsley, P.J., Collins, G.G. and Sedgley, M. 2001. In vitro rooting of almond (Prunus dulcis Mill.). In vitro Cellular and Developmental Biology – Plant, 37: 778-785.

Anderson, W. 1978. Rooting of tissue cultured propagation of rhododendron. In vitro, 40: 334. Bouza, L. 1997. Micropropagation of Prunus tenella (dwarf Russian almond). pp. 276-288. In: Biotechnology in Agriculture and Forestry, Vol. 5 (ed. Y.P.S. Bajaj), Springer-Verlag, Berlin, Heidelberg, Germany.

Chanuntapipat, C., Sedgley, M. and Collins, G. 2003. Micropropagation of almond cultivars Nonpariel and Ne Plus Ultra and the hybrid rootstock Titan x Nemaguard. Scientia Horticulturae, 98: 473- 484.

Dalal, M.A., Kuchey, A.G. and Rather, M.A. 2000. Studies on stock plant treatment and culture initiation in control of browning and media exudation in in vitro cultures of apple (Malus domestica Borkh.). SKUAST Journal of Research, 2: 10-16.

Gurel, S. and Gulsen, Y. 1998. The effects of IBA and BAP on in vitro shoot production of almond (Amygdalus communis L.). Turkish Journal of Botany, 22: 375-379.

Ishidia, M., Matsuyama, H., Kitajima, A. and Sobajima, Y. 1989. In vitro propagation of Prunus Japonica Thumb., A dwarfing rootstock for peach tree. Journal of Japanese Society of Horticultural Science, 58: 49-54.

Isikalan, C., Akbas, F.A., Namli, S.,Tilkat, E. and Davut, B. 2008. In vitro micropropagtion of almond (Amygdalus communis L. cv. Nonpariel). African Journal of Biotechnology, 7: 1875-1880. Kester, D.E., Liu, F., Fenton, C.A.L. and Durzan, D.J. 1986. Almond [Prunus dulcis (Miller) D.A. Webb]. pp 414-430. In: Biotechnology in Agriculture and Forestry, Vol. 1 (ed. Y.P.S. Bajaj), Springer-Verlag, Berlin, Heidelberg. Germany.

M.A. Mir et al.

Livari,B.V. and Soghadi, Z.S. 2005. In vitro rooting of hybrid almond rootstock GF-677 (Prunus dulcis x Prunus persica). Acta Horticulturae, 726: 165-168.

Muna, A.S., Ahmad, A.K., Mahmoud, K. and Abdul Rahman, K. 1999. In vitro propagation of semi dwarfing cherry rootstock. Plant Cell Tissue and Organ Culture, 59: 203-208.

Murashige, T. and Skoog, F. 1962. A revised medium for rapid growth and bioassay with tobacco tissue culture. Physiologia Plantarum, 15: 473-479.

Qadiri, I.H., Kamili, A.N. and Shah, A.M. 2001. Micropropagation of almond. Journal of Research and Development, 1: 111-117.

Qadiri, I.H., Kamili, A.N., Bashir, S. and Shah, A.M. 2002. Shoot tip culture of thin shelled almond. Oriental Science, 7: 95-102.

Rugini, E. and Verma, D. 1983. Micropropagation of difficult-to-propagate almond (P. amygdalus Batsch.) cultivar. Plant Science Letters, 28: 273-281.

Rugini, E. and Verma, D.C. 1982. Micropropagation and cell suspensions of a difficult to propagate almond (Prunus amygdalus Batsch.) cultivar. pp. 741-742. In: Proceedings of 5th International Conference on Plant Tissue and Cell Culture, (ed. A. Fujiwara). The Japanese Association for Plant Tissue Culture, Tokyo, Japan.

Sharma, D.R., Chauhan, P.S., Kaur, R. and Srivastava, D.K. 1992. Micropropagation of colt – A semi dwarf rootstock of cherry. Indian Journal of Horticulture, 49: 209-212.

Shibli, R.A., Joradat, A., Ajlouni, M.M. and Aljanabi, S. 1996. In vitro multiplication of bitter almond (Prunus amygdalus) from north Jordan. In vitro, 32: 32-36.

Sriskandarajah, S., Goodwin, P.B. and Speirs, P. 1994. Genetic transformation of the apple scion cultivar Delicious via Agrobacterium tumefaciens. Plant Cell Tissue and Organ Culture, 36: 317- 329.

Tabachnik, L. and Kester, D.E. 1977. Shoot culture for almond and almond peach hybrid clones in vitro. HortScience, 12: 545-547.