Micropropagation Of Serpgandha (Rauvolfia Serpentina L. Benth):  An Endangered Medicinal Plant

Archana Rani; M Kumar; Sanjeev Kumar

doi:10.48165/

Authors

Archana Rani Department of Agricultural Biotechnology and Molecular Biology, (FBS & H), Rajendra Agricultural University, Pusa - 848 125, Bihar (India)
M Kumar Department of Agricultural Biotechnology and Molecular Biology, (FBS & H), Rajendra Agricultural University, Pusa - 848 125, Bihar (India)
Sanjeev Kumar Department of Plant Pathology, College of Agriculture, Jawaharlal Nehru Krishi Vishwa Vidyalaya, Jabalpur – 482 004, Madhya Pradesh (India)

DOI:

https://doi.org/10.48165/

Keywords:

Endangered species, in vitro propagation, medicinal plant, Rauvolfia serpentine

Abstract

An efficient protocol for propagation of Rauvolfia serpentina through in vitro culture was standardized. Of the various combinations of phyto hormones evaluated, 0.5 mg L-1indole acetic acid (IAA) +0.5 mg L-1 benzyl amino purine (BAP) + 1.0 mg L-1kinetin highly favoured callus induction (32.8%). High callus-mediated shoot regeneration was seen on MS medium supplemented with 1.0 mg L-1IAA + 0.5 mgL-1BAP + 0.5 mg L-1kinetin. Low auxin (IAA, 0.5 mg L-1) with higher concentration of cytokinin (0.5 mgL-1BAP +1.0mg L-1kinetin) gave higher shoots culture-1 (6.6) with mean shoot length of 3.2 cm. In direct shoot regeneration, best growth of axillary shoots was achieved in MS medium supplemented with0.2 mg L-1IAA + 0.2 mg L-1 BAP + 0.2 mg L-1kinetin with maximum shoots culture-1(10.0) and shoot length (4.4 cm). The root induction was highest (84.3%) in MS medium supplemented with IBA (0.5 mg L-1). The rooted plantlets were successfully established in field.

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