In Vitro Regeneration Of Cinnamomum Tamala Nees From  Cotyledonary Segments

Chitta Ranjan Deb; Madhabi S Deb; N Saku Jamir

doi:10.48165/

Authors

Chitta Ranjan Deb Department of Botany, Nagaland University, Lumami 798 627, Nagaland, India
Madhabi S Deb Department of Botany, Nagaland University, Lumami 798 627, Nagaland, India
N Saku Jamir Department of Botany, Nagaland University, Lumami 798 627, Nagaland, India

DOI:

https://doi.org/10.48165/

Keywords:

Cinnamomum tamala, cotyledonary segments, in vitro morphogenetic response, spice yielding plant

Abstract

Cinnamomum tamala Nees., a valuable spice-yielding plant of Himalaya, is vulnerable in its natural habitat due to its over-collection for spice and medicinal purpose. In the present study, a successful attempt was made for in vitro shoot bud induction from cotyledons and plant regeneration. The culturedcotyledons of 10-11wk after flowering induced meristematic response within 4-5 wk of culture where ~43% explants responded positivelyonMSmediumfortifiedwith 3% sucrose, polyvinyl pyrollidone (100mgL-1) and 6 µM benzyl adenine wherein ~5-6 shoot buds developed cotyledon-1.Themicro-shoots/shoot buds,developedon initiation medium, were maintained on MS medium fortified with 3% sucrose and 3 µM kinetin where shoot buds converted into plantlets and supported culture proliferation. Shoots (4 cm long) with fully open leaves were rooted by maintaining on MS medium enriched with 3 µM α-naphthalene acetic acid where 5-6 roots developed per micro-shoot. The rooted plants were transferred to potting mix and maintained in agro-shade ca75% shading where ~65% transplants survived after two months of transfer.

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