Antifertility Effects Of Single Oral Doses Of Triptolide  In Male House Rat (Rattus Rattus L.)

Neena Singla

doi:10.48165/

Authors

Neena Singla Department of Zoology, Punjab Agricultural University, Ludhiana – 141 004, Punjab, India

DOI:

https://doi.org/10.48165/

Keywords:

Antifertility, Rattus rattus, single dose, triptolide

Abstract

The aim of present study was to evaluate the effect of single oral doses of triptolide on fertility of male house rat, Rattus rattus. Single oral administration of triptolide to male R. rattus resulted in 16.67 and 33.33% mortality in rats treated with 100 and 150 mg kg-1b.wt. of triptolide, respectively, within 1-3 days of treatment. The LD50 value of triptolide against male R. rattus was found to be 182.81 mg kg-1 b.wt. Autopsy of rats surviving till day 45 after treatment revealed no significant effect on weights of reproductive organs. The sperm motility and viability in cauda epididymal fluid were reduced significantly in all the treated groups. Sperm concentration was significantly reduced only in rats treated with 100 mg kg-1 b.wt. of triptolide. Treatments significantly affected sperm morphology. The sperms with head tail separation ranged from 15.8 to 24.0% in all treated groups. The present study suggests the potential of triptolide in regulating fertility of male R. rattus.

Downloads

Download data is not yet available.

References

Alvarez, J.G. and Storey, B.T. 1989. Role of glutathione peroxidase in protecting mammalian spermatozoa from loss of motility caused by spontaneous lipid peroxidation. Gamete Research, 23: 77-90.

Aravindan, G.R., Krishnamurthy, H. and Moudgal, N.R. 1997. Enhanced susceptibility of follicle stimulating hormone-deprived infertile bonnet monkey (Macaca radiata) spermatozoa to dithiothreitol induced DNA decondensation in situ. Journal of Andrology, 18: 688-697.

Bernacchi, A.S., de Ferreyra, E.C., de Castro, C.R. and Castro, J.A. 1993. Ultrastructural alterations in testes from rats treated with cysteine. Biomedical Environmental Sciences, 6: 172-178. Brakes, C.R. and Smith, R.H. 2005. Exposure of non-target small mammals to rodenticides, short

term effects, recovery and implications for secondary poisoning. Journal of Applied Ecology, 42: 118-128.

Chen, H., Lu, W.G., Chen, J.F., Xu, Q., Chen, G.X. and Liu, Q.P. 2010. Influence of Tripterygium hypoglaucum Hutch on rat sperms evaluated by computer-assisted sperm analysis system. Journal of Guangzhou University of Traditional Chinese Medicine, 27: 141-146.

Dhar, P. and Singla, N. 2013. Effect of triptolide on reproductive output of male Bandicota bengalensis. International Journal of Advanced Research, 1: 705-716.

Dhar, P. and Singla, N. 2014. Effect of triptolide on reproduction of female lesser bandicoot rat, Bandicota bengalensis. Drug and Chemical Toxicology, Published online, DOI: 10.3109/ 01480545.2014.884111.

Dhar, P., Singla, N. and Babbar, B.K. 2014. Effect of triptolide on vital organs, blood biochemical parameters and histomorphology of testis in male Bandicota bengalensis. Indian Journal of Applied Research, 4: 27-30.

Dubey, N.K., Srivastava, B. and Kumar, A. 2008. Current status of plant products as botanical pesticides in storage pest management. Journal of Biopesticides, 1: 182-186. Faleh, A.B., Annabi, A. and Said, K. 2012. Morphometric variation in black rat Rattus rattus (Rodentia: Muridae) from Tunisia. Acta Zoologica Bulgarica, 64: 381-387. Gandy, J., Primiano, T., Novak, R.F., Kelce, W.R. and York, J.L. 1996. Differential expression of glutathione S-transferase isoforms in compartments of the testis and segments of the epididymis of the rat. Drug Metabolism Disposition, 24: 725-733.

Huynh, P.N., Hikim, A.P., Wang, C., Stefonovic, K., Lue, Y.H., Leung, A., Atienza, V., Baravarian, S., Reutrakul, V. and Swerdloff, R.S. 2000. Long-term effects of triptolide on spermatogenesis, epididymal sperm function and fertility in male rats. Journal of Andrology, 21: 689-699.

Liu, J., Jiang, Z., Liu, L., Zhang, Y., Zhang, S., Xiao, J., Ma, M. and Zhang, L. 2010. Triptolide induces adverse effect on reproductive parameters of female Sprague-Dawley rats. Drug and Chemical Toxicology, 34: 1-7.

Effect of triptolide on male house rats 77

Lue, Y., Sinha Hikim, A.P., Wang, C., Leung, A., Baravarian, S., Reutrakul, V., Sangsawan, R., Chaichana, S. and Swerdloff, R.S. 1998. Triptolide: A potential male contraceptive. Journal of Andrology, 19: 479-486.

Meerburg, B.G. 2009. Rodents are a risk factor for the spreading of pathogens on farms. Veterinary Microbiology, 142: 464-465.

Mineau, P., Shore, R.F. and Hosea, R.C. 2004. Towards a risk assessment of second generation rodenticides: Do we have enough information to proceed? pp. 19-21. In: Proceedings of 2nd National Invasive Rodent Summit. National Wildlife Research Center, LaPorte Avenue, Fort Collins, USA.

Ni, B., Jiang, Z., Huang, X., Xu, F., Zhang, R., Zhang, Z., Tian, Y., Wang, T., Zhu, T., Liu, J. and Zhang, L. 2008. Male reproductive toxicity and toxicokinetics of triptolide in rats. Arzneimittel Forschung, 58: 673-680.

Papp, S., Robaire, B. and Hermo, L. 1995. Immunocytochemical localization of the Ya, Yc, Yb1, and Yb2 subunits of glutathione S-transferases in the testis and epididymis of adult rats. Microscopy Research and Techniques, 30: 1-23.

Salisbury, G.W., VanDenmark, Ν.L. and Lodge, J.R. 1978. Physiology of Reproduction and Artificial Insemination of Cattle. W H Freeman & Co, San Francisco, California, USA. Shilova, S.A. and Tchabovsky, A.V. 2009. Population response of rodents to control with rodenticides. Current Zoology, 55: 81-91.

Singla, L.D., Singla, N., Parshad, V.R., Juyal, P.D. and Sood, N.K. 2008. Rodents as reservoir of parasites in India. Integrative Zoology, 3: 21-26.

Singla, N and Garg, M. 2013. Effect of crude cottonseed oil containing gossypol on fertility of male and estrous cycle of female Bandicota bengalensis Gray and Hardwicke. Journal of Applied Animal Research, 41: 156-165.

Singla, N., Kaur, G., Babbar, B.K. and Sandhu, B.S. 2013a. Potential of triptolide in reproductive management of house rat, Rattus rattus (Linnaeus). Integrative Zoology, 8: 260-276. Singla, N., Singla, L. D., Gupta, K. and Sood, N. K. 2013b. Pathological alterations in natural cases of Capillaria hepatica infection alone and in concurrence with Cysticercus fasciolaris in Bandicota bengalensis. Journal of Parasitic Diseases, 17: 16-20.

Singla, N. and Mittal, M. 2012. Management of post control rodent population rebuildup in sugarcane. Journal of Research Punjab Agricultural University, 49: 60-64.

Sinha Hikim, A.P., Lue, Y.H., Wang, C., Reutrakul, V., Sangsuwan, R. and Swerdloff, R.S. 2000. Post-testicular antifertility action of triptolide in the male rat: evidence for severe impairment of cauda epididymal sperm ultrastructure. Journal of Andrology, 21: 431-437.

Xue, X., Gong, L., Qi, X., Wu, Y., Xing, G., Yao, J., Luan, Y., Xiao, Y., Li, Y., Wu, X., Chen, M., GU, J. and Ren, J. 2011. Knockout of hepatic P450 reductase aggravates triptolide-induced toxicity. Toxicology Letters, 205: 47-54.

Ye, W., Den, Y., Huang, Y. and Xue, S. 1994. Antispermatogenic effect of Tripterygium wilfordii and tripchlorolide (T4) on rat gametogenesis and spermatozoa. Chinese Medical Sciences Journal, 9: 110-111.