Genetic Variability Among Isolates Of Xanthomonas Citri Subsp. Citri Based On Rep-Pcr

Nitish Rattan Bhardwaj; J S Srivastava; Vinod Upadhyay; Dinesh Singh; Mamta Nagar

doi:10.48165/

Authors

Nitish Rattan Bhardwaj Department of Mycology and Plant Pathology, Institute of Agricultural Sciences, Banaras Hindu University, Varanasi – 221 005, Uttar Pradesh (India)
J S Srivastava Department of Mycology and Plant Pathology, Institute of Agricultural Sciences, Banaras Hindu University, Varanasi – 221 005, Uttar Pradesh (India)
Vinod Upadhyay Department of Mycology and Plant Pathology, Institute of Agricultural Sciences, Banaras Hindu University, Varanasi – 221 005, Uttar Pradesh (India)
Dinesh Singh Division of Plant Pathology, Indian Agricultural Research Institute, New Delhi – 110 001 (India)
Mamta Nagar Department of Mycology and Plant Pathology, Institute of Agricultural Sciences, Banaras Hindu University, Varanasi – 221 005, Uttar Pradesh (India)

DOI:

https://doi.org/10.48165/

Keywords:

Canker, citrus, Xanthomonas, rep-PCR

Abstract

Citrus canker caused by Xanthomonas citri subsp. citri is one of the most important disease of citrus in India. In the present study 23 isolates of Xanthomonas citri subsp. citri were collected from Varanasi and Jaunpur districts of Uttar Pradesh (India). Isolates showed similar biochemical characteristics whereas molecular analysis involving repetitive polymerase chain reaction [rep-PCR] (BOX-, ERIC- and REP-PCR) grouped these isolates into six clusters at 50% similarity coefficient, thereby revealing presence of variation in X. citri subsp. citri isolates.

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