Bioprocessing Of Bajra Straw Using Locally Isolated  Aspergillus Niger Hd-6 For Endocellulase Production

D K Acharya; G R  Bhojani; P R Prajapati; H M Patel; R M Shukla

doi:10.48165/

Authors

D K Acharya Biotechnology Department, Anand Mercantile College of Science, Management and Computer Technology, Near New S.T. Station, Bhalej Road, Anand, Gujarat – 388 001 (India)
G R Bhojani Biotechnology Department, Anand Mercantile College of Science, Management and Computer Technology, Near New S.T. Station, Bhalej Road, Anand, Gujarat – 388 001 (India)
P R Prajapati Biotechnology Department, Anand Mercantile College of Science, Management and Computer Technology, Near New S.T. Station, Bhalej Road, Anand, Gujarat – 388 001 (India)
H M Patel Biotechnology Department, Anand Mercantile College of Science, Management and Computer Technology, Near New S.T. Station, Bhalej Road, Anand, Gujarat – 388 001 (India)
R M Shukla Department of Microbiology and Biofertilizer Project, B.A. College of Agriculture, Anand Agriculture University, Anand, Gujarat - 388 001 (India)

DOI:

https://doi.org/10.48165/

Keywords:

Bajra straw, endocellulase, optimization, solid state fermentation

Abstract

Aspergillus niger strain HD-6, isolated from Anand, Gujarat (India), was evaluated for endocellulase enzyme production through solid state fermentation (SSF) using bajra straw as main carbon source. Various cultural conditions such as temperature, pH, moisture content, incubation period, nitrogen source, inoculum age and size, etc. were standardized for optimum enzyme production. Using 3 g bajra straw as sole carbon source maximum endocellulase production from 96 hr old A. niger HD-6 was achieved at pH 5.0, incubation temperature 28°C and moisture content 65% after incubation for 4 days.

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References

Abo-State, M.A.M, Hammad, A.I., Swelim, M. and Gannam, R.B. 2010. Enhanced production of cellulase(s) by Aspergillus spp. isolated from agriculture wastes by solid state fermentation. American-Eurasian Journal of Agricultural and Environmental Sciences, 8: 402-410.

Acharya, D.K., Chabhadiya, S.B., Shah, A.J., Acharya, P.B., Shilpakar, P. and Modi, H.A. 2010. Enzyme profiling of lignocellulolytic fungi. International Journal of Biological and Chemical Science, 4: 443-449.

Ahmed, S., Bashir, A., Saleem, H., Saadia, M. and Jamil, A. 2009. Production and purification of cellulose degrading enzymes from a filamentous fungus Trichoderma harzianum. Pakistan Journal of Botany, 41: 1411-1419.

Bakri, Y., Jacques, P. and Thonart, P. 2003. Xylanase production by Penicillium canescens 10-10c in solid-state fermentation. Applied Biochemistry and Biotechnology, 105: 737-747. Beg, Q.K., Bhushan, B., Kapoor, M. and Hoondal, G.S. 2000. Enhanced production of thermostable xylanase from Streptomyces sp. QG-11-3 and its application in biobleaching of eucalyptus kraft pulp. Enzyme and Microbial Technology, 27: 459-466.

Berg, B., Hofstan, B.V. and Petterson, B. 1972. Growth and cellulase formation by Cellvibrio fulvus. Journal of Applied Bacteriology, 35: 201-214.

Da Silva, R., Lago, E.S., Merheb, C.W., Macchione, M.M., Park, Y.K. and Gomes, E. 2005. Production of xylanase and CMCase on solid state fermentation in different residues by Thermoascus aurantiacusmiehe. Brazilian Journal of Microbiology, 36: 235-241.

Ekperigin, M.M. 2007. Preliminary studies of cellulase production by Acinetobacter anitratus and Branhamella sp. African journal of Biotechnology, 6: 28-33.

Ghose, T.K. 1986. Measurement of cellulase activities. Pure and Applied Chemistry, 59: 257-268. Haq, I., Hameed, U., Shahzadi, K, Javed, M.M., Ali, S. and Qadeer, M.A. 2005. Cotton saccharifing activity of cellulases by Trichoderma harzianum UM-11 in shake flask. International Journal of Botany, 1: 19-22.

Haq, I., Javed, M.M. and Khan, T.S. 2006. An innovative approach for hyperproduction of cellulolytic and hemicellulolytic enzymes by consortium of Aspergillus niger MSK-7 and Trichoderma viride MSK-10. African journal of Biotechnology, 5: 609-614.

Kang, S.W., Park, Y.S., Lee, J.S., Hong, S.I. and Kim, S.W. 2004. Production of cellulase and hemicellulase by Aspergillus niger KK2 from lignocellulosic biomass. Bioresource Technology, 91: 153-156.

Kapdan, L.K. and Kargi, F. 2006. Biohydrogen production from waste materials. Enzyme and Microbial Technology, 38: 569-582.

Khan, M.D., Ali, M.H., Fakhru’l-Razi, A.S. and Alam, M.D.Z. 2007. Use of fungi for bioconversion of rice straw into cellulase enzyme. Journal of Environmental Science and Health Part B, 42: 381-386.

Kheng, P.P. and Omar, I.C. 2005. Xylanase production via solid state fermentation. Journal of Science and Technology, 27: 332.

Kim, J. and Yun, S. 2006. Discovery of cellulose as a smart material. Macromolecules, 39: 4202- 4206.

Kim, K.C., Yoo, S.S., Oh, Y.A. and Kim, S.J. 2003. Isolation and characteristics of Trichoderma harzianum FJ1 producing cellulases and xylanase. Journal of Microbiology and Biotechnology, 13: 1-8.

Kirk, T.K., Tien, M., Kersten, P.J., Kalyanaraman, B., Hammel, K.E., Farrell, R.L. 1990. Lignin peroxidase from fungi: Phanerochaete chrysosporium. Methods in Enzymology, 188: 159- 171.

Bioprocessing of bajra straw for endocellulase production 231

Kuhad, R.C., Singh, A. and Eriksson, K.E.L. 1997. Microorganisms enzymes involved in the degradation of plant fiber cell walls. Advances in Biochemical Engineering/Biotechnology, 57: 45-125.

Lee, S.M. and Koo, Y.M. 2001. Pilot-scale production of cellulase using T. reesei rut C-30 in fed batch mode. Journal of Microbiology and Biotechnology, 11: 229-233.

Mandels, M. and Sternburg, D. 1976. Recent advances in cellular technology. Journal of Fermentation Technology, 54: 267-286.

Mandels, M. and Weber, J. 1969. Production of cellulases. Advances in Chemistry Series, 95: 391- 414.

Manga, V.K. and Kumar, A. 2011. Cultivar options for increasing pearl millet productivity in arid regions. Indian Journal of Fundamental and Applied Life Sciences, 1: 200-208. Miller, G.L. 1959. Use of dinitrosalicylic acid reagent for determination of reducing sugars. Analytical Chemistry, 31: 426-428.

Nochure, S.V., Roberts, M.F. and Demain, A.L. 1993. True cellulase production by Clostridium thermocellum grown on different carbon sources. Biotechnology Letters, 15: 641-646. Ojumu, T.V., Solomon, B.O., Betiku, E., Layokun, S.K. and Amigun, B. 2003. Cellulase production by Aspergillus flavus Linn isolate NSPR 101 fermented in sawdust, bagasse and corncob. African journal of Biotechnology, 2: 150-152.

Ong, L.G.A., Chuah, C. and Chew A.L. 2010. Comparison of sodium hydroxide and potassium hydroxide followed by heat treatment on rice straw for cellulase production under solid state fermentation. Journal of Applied Sciences, 10: 2608-2612.

Rangaswami R. 1995. A Text Book of Agricultural Statistics. New Age International Pub. Ltd., New Delhi, India.

Rudravaram, R., Chandel, A.K., Linga, V.R. and Pogaku, R. 2006. Optimization of protein enrichment of deoiled rice bran by solid state fermentation using Aspergillus oryzae MTCC 1846. International Journal of Food Engineering, 2(4): 1-14.

Shah, I.J., Gami, P.N., Shukla, R.M. and Acharya, D.K. 2014. Optimization for α-amylase production by Aspergillus oryzae using submerged fermentation technology. Basic Research Journal of Microbiology, 1(4): 1-10.

Sharma, D.K., Tiwari, M. and Behere, B.K. 1996. Solid state fermentation of new substrates for production of cellulase and other biopolymer hydrolyzing enzymes. Applied Biochemistry and Biotechnology, 15: 495-500.

Topakas, E., Katapodis, P., Kekos, D., Macris, B.J. and Christakopoulos, P. 2003. Production and partial characterization of xylanase by Sporotrichum thermophile under solid-state fermentation. World Journal of Microbiology and Biotechnology, 19: 195-198.

Xin F. and Geng A. 2010. Horticulture waste as the substrate for cellulase and hemicellulase production by Trichoderma reesei under solid state fermentation. Applied Biochemistry and Biotechnology, 162: 295-306.