Biochemical Defense Induction In Indian Mustard (Brassica  Juncea L.) And Rapeseed (B. Napus L.) By Salicylic Acid And  Benzothiadiazole

Meenakshi Thakur; B S Sohal

doi:10.48165/

Authors

Meenakshi Thakur Department of Biochemistry, College of Basic Sciences and Humanities, Punjab Agricultural University, Ludhiana – 141 004, Punjab (India)
B S Sohal Department of Biochemistry, College of Basic Sciences and Humanities, Punjab Agricultural University, Ludhiana – 141 004, Punjab (India)

DOI:

https://doi.org/10.48165/

Keywords:

Ascorbic acid, β-1, 3-glucanase, benzothiadiazole, Brassica, chitinase, chlorophyll, salicylic acid

Abstract

Alternaria blight, caused by Alternaria brassicae (Berk.) Sacc., is one of the serious diseases of Brassica species. A field experiment was conducted to evaluate the effects of two elicitors, salicylic acid (SA) and benzothiadiazole (BTH), on defense response induction in Brassica juncea and B. napus against Alternaria blight. Four different treatments of SA and BTH were given to 10 week old plants upto 4 consecutive weeks. Chitinase and β-1,3- glucanase enzyme activities; and ascorbic acid and pigment contents were assessed 72 hr after treatment and disease incidence recorded a week after spray. Hydrolytic enzymes activities attained maximum level after 2nd spray in treatment combinations BTH (3 ppm) + SA (33 ppm) and BTH (7 ppm) + SA (17 ppm). An increase in ascorbic acid and pigment contents was observed in all the treatments after 1st, 2nd and 3rd spray followed by a decrease after 4th spray. Maximum increase was seen in treatment combinations of elicitors. These treatments also reduced Alternaria blight severity in both the species. The elicitors, applied in combinations, showed synergistic effect on the induction of hydrolytic enzyme activities as well as ascorbic acid and pigment contents in comparison to the individual applications. The study showed that the elicitor combinations can be used effectively to combat Alternaria blight in Brassica species.

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References

Abdel-Monaim, M.F., Ismail, M.E. and Morsy, K.M. 2011. Induction of systemic resistance in soybean plants against Fusarium wilt disease by seed treatment with benzothiadiazole and humic acid. Notulae Scientia Biologicae, 3: 80-89.

Induction of defense in Brassica species by elicitors 207

Amaresh, C. and Bhatt, R.K. 1998. Biochemical and physiological response to salicylic acid in relation to the systemic acquired resistance. Photosynthetica, 35: 255-258.

Babu, R.M., Sajeena, A., Vijayasamundeeswari, A., Sreedhar, A., Vidhyasekeran, P. and Reddy, M.S. 2003. Induction of bacterial blight (Xanthomonas oryzae pv. oryzae) resistance in rice by treatment with acibenzolar-S-methyl. Annals of Applied Biology, 143: 333-340.

Boller, T. and Mauch, F. 1988. Colorimetric assay for chitinase. Methods in Enzymology, 161: 430- 435.

Cavalcanti, F.R., Resende, M.L.V., Ribeiro, J.P.M., Pereira, R.B. and Oliveira, J.T.A. 2008. Induction of resistance against Verticillium dahliae in cacao by a Crinipellis perniciosa suspension. Journal of Plant Pathology, 90: 273-280.

Chaudhary, M.H.Z., Sarwar, N. and Chaughati, F.A. 2001. Biochemical changes in chickpea plant after induction treatment with simple chemical for systemic resistance against Ascochyta blight in the field. Journal of Chemical Society of Pakistan, 23: 182-186.

Cheng-Peng and Li Zhen Qi. 2006. BTH systemic induction to defense related enzymes in wheat leaves. Acta Botanica Boreali Occidentalia Sinica, 26: 2468-2472.

Cohen, Y., Reuveni, M. and Baider, A. 1999. Local and systemic activity of BABA (DL-3- aminobutyric acid) against Plasmopara viticola in grapevines. European Journal of Plant Pathology, 105: 351-361.

Conn, K.L., Tiwari, J.P. and Awasthi, R.P. 1990. A disease assessment key for Alternaria black spot in rapeseed and mustard. Canadian Plant Disease Survey, 70: 19-22.

Davies, B.H. 1976. Carotenoids. pp. 154. In: Chemistry and Biochemistry of Plant Pigments. (Ed. T.W. Goodwin). Academic Press, London, UK.

El-Gamal, N.G., Abd-El-Kareem, Fotouh, Y.O. and El-Mougy, N.S. 2007. Induction of systemic resistance in potato plants against late and early blight diseases using chemical inducers under green house and field conditions. Research Journal of Agriculture and Biological Sciences, 3: 73-81.

El-Hendawy, S., Shaban, W. and Sakagami, J.I. 2010. Does treating faba bean seeds with chemical inducers simultaneously increase chocolate spot disease resistance and yield under field conditions? Turkish Journal of Agriculture and Forestry, 34: 475-485.

Farouk, S. and Osman, M.A. 2012. Alleviation of oxidative stress induced by spider mite invasion through application of elicitors in bean plants. Egyptian Journal of Biology, 14: 1-13. Jaleel, C.A., Gopi, R. and Panneerselvam. 2009. Alterations in non-enzymatic antioxidant components of Catharanthus roseus exposed to paclobutrazol, gibberellic acid and Pseudomonas fluorescens. Plant Omics Journal, 2: 30-40.

Karthikeyan, M., Jayakumar, V., Radhika, K., Bhaskaran, R., Velazhahan, R. and Alice, D. 2005. Induction of resistance in host against the infection of leaf blight pathogen (Alternaria palandui) in onion (Allium cepa var aggricatum). Indian Journal of Biochemistry and Biophysics, 42: 371-377.

Kauffmann, S., Legrand, M., Geoffroy, P. and Fritig, B. 1987. Biological function of pathogenesis related proteins: Four PR proteins of tobacco have 1,3-β-glucanase activity. The EMBO Journal, 6: 3209-3212.

Leah, R., Tommerup, H., Svendsen, I. and Mundy, J. 1991. Biochemical and molecular characterization of three barley seed proteins with antifungal properties. Journal of Biological Chemistry, 266: 1564-1573.

Lee, Y.S., Kim, Y.H. and Kim, S.B. 2005. Changes in the respiration, growth, and vitamin C content of soybean sprouts in response to chitosan of different molecular weights. HortScience, 40: 1333-1335.

Legrand, M., Kauffmann, S., Geoffroy, P. and Fritig, B. 1987. Biological function of pathogenesis related proteins: Four tobacco pathogenesis-related proteins are chitinases. Proceedings of the National Academy of Sciences of the United States of America, 84: 6750-6754.

Meenakshi Thakur and B.S. Sohal

Mandavia, M.K., Raval, L., Mandavia, C. and Karkar, C. 2012. Effect of brassinolide and salicylic acid on biochemical parameters and yield of groundnut. Indian Journal of Agricultural Biochemistry, 25: 20-24.

Meena, P.D., Awasthi, R.P., Chattopadhyay, C., Kolte, S.J. and Kumar, A. 2010. Alternaria blight: A chronic disease in rapeseed-mustard. Journal of Oilseed Brassica, 1: 1-11. Meena, P.D., Meena, R.L., Chattopadhyay, C. and Kumar, A. 2004. Identification of critical stage for disease development and biocontrol of Alternaria blight of Indian mustard (Brassica juncea). Journal of Phytopathology, 152: 204-209.

Patni, C.S. and Kolte, S.J. 2006. Effect of some botanicals in management of Alternaria blight of rapeseed-mustard. Annual Journal of Plant Protection Science, 14: 151-156. Reissig, R., Dor, G. and Reuveni, M. 1955. A modified colorimetric method for the estimation of N-acetylamine sugars. Journal of Biological Chemistry, 217: 959-966.

Roe, J.H. and Kuether, C.A. 1943. The determination of ascorbic acid in whole blood and urine through the 2, 4-dinitrophenyl hydrazine derivative of dehydroascorbic acid. Journal of Biological Chemistry, 147: 399-407.

Singh, A. 1977. Practical Plant Physiology. Kalyani Publishers, New Delhi. India. Smirnoff, N., Conkin, P.L. and Loewus, F.A. 2001. Biosynthesis of ascorbic acid in plants: A renaissance. Annual Review of Plant Physiology and Plant Molecular Biology, 52: 437-467. Walters, D.R., Paterson, L. and Havis, N.D. 2010. Control of foliar diseases of spring barley using resistance elicitors. pp. 91-96. In: Proceedings of Crop Protection, Northern Britain. Yao, H.J. and Tian, S.P. 2005. Effects of pre- and post-harvest application of salicylic acid or methyl jasmonate on inducing disease resistance of sweet cherry fruit in storage. Postharvest Biology and Technology, 35: 253-262.

Yildirim, E., Turan, M. and Guvenc, I. 2008. Effect of foliar salicylic acid applications on growth, chlorophyll and mineral content of cucumber (Cucumis sativus L.) grown under salt stress. Journal of Plant Nutrition, 31: 593-612.