Molecular Detection Of Mycoplasma Spp. Associated With  The Female Reproductive Tract Of Cattle And Buffaloes

Paviter Kaur; N S Sharma; A K Arora; T S Rai

doi:10.48165/

Authors

Paviter Kaur Department of Veterinary Microbiology, College of Veterinary Science, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana - 141 004, Punjab (India)
N S Sharma Department of Veterinary Microbiology, College of Veterinary Science, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana - 141 004, Punjab (India)
A K Arora Department of Veterinary Microbiology, College of Veterinary Science, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana - 141 004, Punjab (India)
T S Rai Department of Veterinary Microbiology, College of Veterinary Science, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana - 141 004, Punjab (India)

DOI:

https://doi.org/10.48165/

Keywords:

Buffaloes, cattle, Mycoplasma, PCR, uterine discharges, vaginal mucus

Abstract

The present study was aimed to detect Mycoplasma spp. associated with the female reproductive tract of cattle and buffaloes in Punjab (India). Out of 65 samples comprising of uterine discharges, vaginal mucus and foetal stomach contents, 21 were positive for Mycoplasma spp. by genus specific PCR using primers based on 16S rDNA of Mycoplasma spp. An amplification band of 270-bp was obtained in all the positive samples. The overall prevalence of Mycoplasma spp. in cattle and buffaloes was 32.3% whereas the prevalence was 24.61 and 7.69% in cattle and buffaloes, respectively. Maximum prevalence of 21.7% was seen in HF breed of cattle and 15.78% in Murrah breed of buffaloes. Cattle and buffaloes in age group 4-6 years and 6-8 years were found more positive for Mycoplasma spp.

Downloads

Download data is not yet available.

References

Amin, S., Rahman, S.U., Hussain, I. and Muhammad, G. 2013. Seroprevalence of Mycoplasma ovipneumoniae among sheep from different districts of Baluchistan, Pakistan. International Journal of Agriculture and Biology, 15: 1043-1046.

Buzinhani, M., Metiffogo, E. and Timenetsky, J. 2007. Detection of Mycoplasma spp. and Ureaplasma diversum in cows with reproductive disorders. Arquivo Brasileiro de Medicina Veterinaria e Zootecnia, 59(6): [http://dx.doi.org/10.1590/S0102-09352007000600003].

Cai, H.Y., Bell-Rogers, P., Parker, L. and Prescott, J.F. 2005. Development of a real-time PCR for detection of Mycoplasma bovis in bovine milk and lung samples. Journal of Veterinary Diagnostic Investigation, 17: 537-545.

Chaudhary, R., Sutikshan, S., Sabah, J., Kapil P., Dey, A.B. and Malhotra P. 2013. Molecular detection of Mycoplasma pneumoniae by quantitative real-time PCR in patients with community acquired pneumonia. Indian Journal of Medical Research, 138: 244-251.

Edward, D.G.F.F. and Fitzgerald, W.A. 1952. A growth factor needed to isolate organisms of the pleuropneumonia group from the genital tract of cattle. Veterinary Record, 64: 395. Kirkbride, C.A. 1987. Mycoplasma, Ureaplasma and Acholeplasma infections of bovine genitalia. Veterinary Clinics of North America: Food Animal Practice, 3: 575-591.

Langford, E.V. 1975. Mycoplasma species recovered from the reproductive tracts of Western Canadian cows. Canadian Journal of Comparative Medicine, 39: 133-138.

Paviter Kaur et al.

Marois, C., Dufour-Gesbert, F. and Kempf, I. 2000. Detection of Mycoplasma synoviae in poultry environment samples by culture and polymerase chain reaction. Veterinary Microbiology, 73: 311-318.

Marouf, S.A., Mohamed, K.F. and Jakea, J.El. 2011. Detection of Mycoplasma bovis and M.bovigenitalium in cattle and buffaloes in Egypt using Dot-ELISA and PCR with anti microbial trials. European Journal of Biological Sciences, 3: 1-8.

Metwally, M.A., Yassin, A.S., Essam, T.M., Hamouda, H.M. and Amin, M.A. 2014. Detection, characterisation, and molecular typing of human Mycoplasma spp. from major hospitals in Cairo, Egypt. Scientific World Journal, [http://dx.doi.org/10.1155/2014/549858].

Mohammed, E.G., Hidetoshi, H., Erisa, T., Hideki, I., Bhuminand, D., Yoshiaki, I. and Takeshi O. 2013. Mycoplasma infection in the uterus of early postpartum dairy cows and its relation to dystocia and endometritis. Theriogenology, 79: 180-185.

Naher, H.S. and Said I.H. 2013. Culturing and PCR methods for detection of Mycoplasma hominis and Ureaplasma urealyticum in women with genitourinary tract infections. International Research Journal of Medical Sciences, 1(3): 25-29.

Nakamura, R.M., Waltc, M.L. and Bennettd, R.H. 1977. Studies of bovine genital tracts: mycoplasma isolations from dairy cows. Theriogenology, 7: 351–355.

Panangala, V.S., Fish, N.A. and Barnum, D.A. 1978. Microflora of the cervico-vaginal mucus of repeat breeder cows. Canadian Veterinary Journal, 19: 83–89.

Perumal, P., Kiran,. K.T. and Srivastava, S.K. 2013. Infectious causes of infertility in buffalo bull (Bubalus bubalis). Buffalo Bulletin, 32(2): 71-96.

Petit T., Spergser J., Aurich J. and Rosengarten R. 2008. Examination of semen from bulls at five Austrian artificial insemination centres for chlamydiae and mollicutes. Veterinary Record, 162: 792–793.

Petit, T., Spergser, J., Aurich, J. and Rosengarten, R. 2008. Prevalence of Chlamydiaceae and Mollicutes on the genital mucosa and serological findings in dairy cattle. Veterinary Microbiology, 27: 325–333.

Pfutzner, H. and Sachse, K. 1996. Mycoplasma bovis as an agent of mastitis, pneumonia, arthritis and genital disorders in cattle. Revue Scientifque Et Technique De L Office International Des Epizooties. 15: 1477-1494.

Qasem, J.A., Mouqati, S.A.A., Ali, E.M.A. and Haji, A.B. 2015. Application of molecular and serological methods for rapid detection of Mycoplasma gallisepticum infection (avian mycoplasmosis). Pakistan Journal of Biological Sciences, 18: 281-287.

Singh, Y., Garg, D.N. and Kapoor, P.K. 2004. Isolation of Mycoplasma bovoculi from genitally diseasesd bovines and its experimental pathogenicity in experimental guinea pigs. Indian Journal of Experimental Biology, 42: 933-936.

Trichard, C.J. and Jacobsz, E.P. 1985. Mycoplasmas recovered from bovine genitalia, aborted foetuses and placentas in the Republic of South Africa. Onderstepoort Journal of Veterinary Research, 52: 105–110.

Yassin, M.H., Al-Humianay, A.A. and Mansour, A.M.A. 2012. Colistin sulphate as a substitute of thallium acetate in culture media for isolation of Mycoplasma from cattle diseased by Mycoplasmosis. International Journal of Microbiology Research, 3: 128-132.