Anti-Reproductive Effects Of Total Glycosides Of  Tripterygium Wilfordii In Male House Rat, Rattus Rattus

Neena Singla

doi:10.48165/

Authors

Neena Singla Department of Zoology, Punjab Agricultural University, Ludhiana - 141 004, Punjab (India)

DOI:

https://doi.org/10.48165/

Keywords:

Anti-reproductive effects, glycosides, Rattus rattus, Tripterygium wilfordii

Abstract

Present study was conducted to evaluate anti-reproductive effects of total glycosides of Tripterygium wilfordii (GTW) (Thunder God Vine), in male House rat, Rattus rattus, a predominant rodent pest species found in commensal situations in Punjab, India. Mature male rats were fed on bait (cracked wheat and powdered sugar, 98:2) containing 0.1, 0.2 and 0.3% GTW for 10 days in no-choice feeding tests. Results revealed 67.2- 85.2% acceptability of treated bait over untreated bait. Autopsy of rats after 30 days of treatment withdrawal revealed significant (P≤0.05) reduction in weights of testes and epididymides. The sperm motility, live sperm count and sperm concentration in the cauda epididymal fluid of treated rats was also reduced significantly compared to that in untreated rats. The major abnormality in sperm morphology was sperm head tail separation. Results suggest the use of GTW for reducing reproduction of rattus in integration with chemical control.

Downloads

Download data is not yet available.

References

Alvarez, J.G.. and Storey, B.T. 1989. Role of glutathione peroxidase in protecting mammalian spermatozoa from loss of motility caused by spontaneous lipid peroxidation. Gamete Research, 23: 77-90.

Aravindan, G.R., Krishnamurthy, H. and Moudgal, N.R. 1997. Enhanced susceptibility of follicle stimulating hormone-deprived infertile bonnet monkey (Macaca radiata) spermatozoa to dithiothreitol induced DNA decondensation in situ. Journal of Andrology, 18: 688-697.

Neena Singla

Chambers, L.K., Ldwson, M.A. and Hinds, L.A.1999. Biological control of rodents - The care for fertility control using immunocontraception. pp. 215-242. In: Ecologically based Rodent Pest Management (eds. G.R. Singleton, L.A. Hinds, H. Leirs and Z. Zhang).ACIAR, Canberra, Australia.

Chen, H., Lu, W.G., Chen, J.F., Xu, Q., Chen, G.X. and Liu, Q.P. 2010. Influence of Tripterygium hypoglaucum Hutch on rat sperms evaluated by computer-assisted sperm analysis system. Journal of Guangzhou University of Traditional Chinese Medicine, 27: 141-146.

Dhar, P. and Singla, N. 2013. Effect of triptolide on reproductive output of male Bandicota bengalensis. International Journal of Advanced Research, 1: 705-716.

Dubey, N.K., Srivastava, B. and Kumar, A. 2008. Current status of plant products as botanical pesticides in storage pest management. Journal of Biopesticides, 1: 182-186. Franken, R.J. 1998. The clinical significance of sperm zona pellucida binding front. Bio Science, 13: 247-253.

Gandy, J., Primiano, T., Novak, R.F., Kelce, W.R. and York, J.L. 1996. Differential expression of glutathione S-transferase isoforms in compartments of the testis and segments of the epididymis of the rat. Drug Metabolism Disposition, 24: 725-733.

Huynh, P.N., Hikim, A.P., Wang, C., Stefonovic, K., Lue, Y.H., Leung, A., Atienza, V., Baravarian, S., Reutrakul, V. and Swerdloff, R.S. 2000. Long-term effects of triptolide on spermatogenesis, epididymal sperm function and fertility in male rats. Journal of Andrology, 21: 689-699.

Matlin, S.A., Belenguer, A., Stacey, Y.E., Qian, S.Z., Xu, Y., Zhang, J.W., Sanders, J.K.M., Amor, S.R. and Pearce, C.M. 1993. Male antifertility compounds from Triprerygium wilfordii Hook F. Contraception, 47: 387-400.

Meerburg, B.G. 2009. Rodents are a risk factor for the spreading of pathogens on farms. Veterinary Microbiology, 142: 464-465.

Mulungu, L.S., Lagwen, P.P., Mdangi, M.E., Kilonzo, B.S. and Belmain, S.R. 2014. Impact of spatiotemporal simulations of rat damage on yield of rice (Oryza sativa L.) and impli cations for rodent pest management. International Journal of Pest Management, 60: 269-274.

Qian, S.Z., Xu, Y. and Zhang, J.W. 1995. Recent progress in research on Tripterygium: A male antifertility plant. Contraception, 51: 121-129.

Shilova, S.A. and Tchabovsky, A.V. 2009. Population response of rodents to control with rodenticides. Current Zoology, 55: 81-91.

Singla, L.D., Singla, N., Parshad, V.R., Juyal, P.D. and Sood, N.K. 2008. Rodents as reservoir of parasites in India. Integrative Zoology, 3: 21-26.

Singla, N. 2014. Antifertility effects of single oral doses of triptolide in male house rat (Rattus rattus L.) Applied Biological Research, 16: 72-74.

Singla, N. and Babbar, B.K. 2010. Rodent damage and infestation in wheat and rice crop fields: District-wise analysis in Punjab state. Indian Journal of Ecology, 37: 184-188. Singla, N. and Challana, S. 2014. Reproductive toxicity of triptolide in male house rat, Rattus rattus. The Scientific World Journal, [Article ID 879405, 6 pages] http://dx.doi.org/ 10.1155/2014/879405.

Singla, N and Garg, M. 2013. Effect of crude cottonseed oil containing gossypol on fertility of male and estrous cycle of female Bandicota bengalensis Gray and Hardwicke. Journal of Applied Animal Research, 41: 156-165.

Singla, N., Kaur, G., Babbar, B.K. and Sandhu, B.S. 2013a. Potential of triptolide in reproductive management of house rat, Rattus rattus (Linnaeus). Integrative Zoology, 8: 260-276. Singla, N., Singla, L.D., Gupta, K. and Sood, N.K. 2013b. Pathological alterations in natural cases of Capillaria hepatica infection alone and in concurrence with Cysticercus fasciolaris in Bandicota bengalensis. Journal of Parasitic Diseases, 17: 16-20.

Suarez, S. S. l996. Hyperactivated motility in sperm. Journal of Andrology, 17: 331-335.