Distribution Of Phosphatases, Oxidoreductases And  Esterases In Sublingual Salivary Gland Of Buffalo  During Neonatal Development

A D Singh; Opinder Singh

doi:10.48165/

Authors

A D Singh Department of Veterinary Anatomy, College of Veterinary Science, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana, Punjab 141 004 (India)
Opinder Singh Department of Veterinary Anatomy, College of Veterinary Science, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana, Punjab 141 004 (India)

DOI:

https://doi.org/10.48165/

Keywords:

Buffalo, histoenzymic, neonates, sublingual salivary gland

Abstract

The present study was aimed to elucidate the distribution of various enzymes in sublingual salivary gland of neonatal buffalo (n = 10), ranging from 1 to 3 months age, and correlate these enzyme systems with the secretory activity of gland. At 1 month age, a uniform moderate to strong positive diffused granular alkaline phosphatase activity was observed in serous acini and ductal epithelium. A moderate positive glucose-6-phosphatase and succinic dehydrogenase activity was seen close to the basement membrane. At 2 months age, glutamic dehydro genase showed moderate activity in basal portions of serous acinar cells, while succinic dehydrogenase activity was strong, granular and evenly distributed in basement membrane of serous cells. Weak activity of monoamine oxidase and moderate non-specific esterase activity was seen. At 3 months age, alkaline phosphatase showed strong positive perinuclear activity in acinar cells, while glucose-6-phosphatase showed a moderate to strong activity in acinar cells and in ductal epithelium. Moderate to strong positive non-specific esterase activity was localized in nerve fibres of intralobular and interlobular connective tissues. Glucose-6-phosphate dehydrogenase and nicotinamide adenine dinucleotide diaphorase showed strong positive granular activity in basement membrane of serous acinar cells and ducts. The high basal activities of oxidoreductases were because of basal mitochondria and were associated with high energy production to meet the energy

Downloads

Download data is not yet available.

References

Asgah, N.A., Jarrar, B.M. and Taib, N.T. 1990. Structure and histochemistry of the sublingual salivary glands of the one-humped camel (Camelus dromedarius). Revue d Elevage et de Medecine Veterinaire des Pays Tropicaux, 43: 519-527.

Aspinall, V. and Reilly, M.O. 2004. Introduction to Veterinary Anatomy and Physiology. An Imprint of Elsevier Ltd., London, UK.

Barka, T. and Anderson, P.J. 1963. Histochemistry: Theory, Practice and Bibliography. Harper & Row Publishers, New York, USA.

Chayen, J., Bitensky, L., Butcher, R.G. and Poulter, L.W. 1969. A Guide to Practical Histo chemistry. Oliver and Boyol, Edinburgh, England.

Featherstone, J.D. 2000. The science and practice of caries prevention. Journal of American Dental Association, 131: 887-889.

Konig, H.E. and Liebich, H.G. 2004. Veterinary Anatomy of Domestic Animals: Textbook and Colour Atlas (1st edn.). Schattauer Co., Stuttgart, Germany.

A.D. Singh and Opinder Singh

Moghaddam, Y.F., Darvish, J., Mahdavi, S.N., Abdulamir, A.S., Mousavi, M. and Daud, S.K. 2009. Comparative histological and histochemical inter-species investigation of mammalian submandibular salivary glands. Research Journal of Applied Science, 4: 50-56.

Narasimhan E., Gopinath S., Rao, K.V.S. and Chandrasekhararao, T.S. 1999. Histological studies on the lingual glands of the goat (Capra hircus). Indian Journal of Veterinary Anatomy, 11: 115-118.

Parkkila, S., Kunisto, K., Rajaniemi, L., Kumpulainen, T., Jokinen, K. and Rajaniemi, H. 1990. Immunohistochemical localization of carbonic anhydrase isonezymes in human parotid and sub-mandibular glands. Journal of Histochemistry and Cytochemistry, 38: 941-947.

Pearse, A.G.E. 1972. Histochemistry: Theoretical and Applied. Vol II. (3rd edn.), Churchill, Livingstone, London.

Pospieszny, N., Kuryszko, J., Juszczyk, M. and Adamski, M. 2010. Morphological and histological analysis of the mandibular gland and sublingual glands in prenatal period of the pig. Bulletin of the Veterinary Institute in Pulawy, 54: 351-355.

Singh, A.D. and Singh, O. 2014. Histoenzymic studies on parotid salivary gland of buffalo during prenatal development. Indian Journal of Veterinary Anatomy, 26: 110-112. Singh, A.D. and Singh, O. 2014. Histoenzymatic studies on prenatal development of submandibular salivary gland in buffalo (Bubalus bubalis), Veterinary World, 7: 1032-1036. Zhou, J., Wang, H., Yang, G., Wang, X., Sun, Y., Song, T., Zhang, C. and Wang, S. 2010. Histological and ultrastructural characterization of developing miniature pig salivary glands. The Anatomical Record, 293: 1227-1239.