Seasonal Variation In Glutathione Peroxidase In Seminal Plasma Of Karan Fries (Tharparkar X Holstein Friesian) Bulls Under Tropical Climatic Conditions
DOI:
https://doi.org/10.48165/Keywords:
Karan Fries bulls, glutathione peroxidase, seasonsAbstract
The present study was aimed to assess the seasonal variation in glutathione peroxidase (GPx) in seminal plasma of Karan Fries (Tharparkar x Holstein Friesian) bulls. Weekly ejaculates were collected by artificial vagina (42-45°C) during winter (December to mid February), spring (mid February-April), hot dry (May-June) and hot humid(July-August) seasons. Higher concentration of GPx was observed during hot humid season (65.74±0.93 pg mL-1) than in winter (59.30± 0.60 pg mL-1), spring (60.67±0.76 pg mL-1) and hot dry (62.14±0.90 pg mL-1) seasons. The percentage of live spermatozoa decreased during hot humid season as compared to other seasons. The acrosomal integrity also decreased during hot dry (78.7±0.6%) and hot humid (74.2 ±0.6%) seasons as compared to winter (83.1±0.6%) and spring (83.4±0.5%) seasons. The supplementation of antioxidant appears essential to Karan Fries bulls during summer season under tropical climatic conditions.
Downloads
References
Aitken, R.J. and Roman, S.D. 2008. Antioxidant systems and oxidative stress in the testes. Oxidative Medicine and Cellular Longevity 1: 15-24.
Barrios, B., Ferna´ndez-Juan, M., Muino-Blanco, T. and Cebria´n-Pe´rez, J. 2005. Immunocyto- chemical localization and biochemical characterization of two seminal plasma proteins that protect ram spermatozoa against cold shock. Journal of Andrology, 26: 539-549.
Barth, A.D. and Bowman, P.A. 1994. The sequential appearance of sperm abnormalities after scrotal insulation or dexamethasone treatment in bulls. Canine Veterinary Journal, 34: 93-102. Bhakat M., Mohanty, T.K., Gupta, A.K. and Abdullah, M. 2014. Effect of season on semen quality of crossbred (Karan Fries) bulls. Advance Animal Veterinary Science, 2: 632-637. Bhuiyan, M.M.U. and Shamsuddin, M. 1998. Bull sperm morphology in context of ambient temperature during semen collection. Bangladesh Veterinary Journal, 32: 33-39. Blom, E. 1950. A rapid staining method using to distinguish between live and dead spermatozoa. Animal Breeding Abstract, 18: 1390.
Simson Soren and Sohan Vir Singh
Brown, D.V., Senger, P.L., Stone, S.L., Froseth, J.A. and Becker, W.C. 1977. Glutathione peroxidase in bovine semen. Journal of Reproduction and Fertility, 50: 117-118.
Cardozo, J., Ferna’ndez-Juan, M., Forcada F., Abecia A., Muiño-Blanco, T., Cebria’n-Pe’rez, J. 2006. Monthly variations in ovine seminal plasma proteins analyzed by two-dimensional polyacrylamide gel electrophoresis. Theriogenology, 66: 841-850.
Chaudhry, M.Z., McDowell, R.E. and Shah, S.K. 1990. Productive and reproductive performance of three generations of Holstein × Sahiwal. pp: 29-39. In: 11th Annual Report. L.P.R.I., Bahadarnagar, Okara, Pakistan.
Chow, C. and Tappel, A.L. 1972. An enzymatic protective mechanism against lipid peroxidation damage to lungs of ozone-exposed rats. Lipids, 7: 518-524.
Combs, G.F., Noguchi, T. and Scott, M.L. 1975. Mechanism of action of selenium and vitamin E in protection of biological membrane. Federation of American Societies for Experimental Biology, 34: 2090-2095.
Fiaz, M., Usmani, R.H., Abdullah, M. and Ahmad, T. 2010. Evaluation of semen quality of Holstein Friesian and Jersey bulls maintained under subtropical environment. Pakistan Veterinary Journal, 30: 75-78.
Flohé, L. and Zimmerman, R. 1970. The role of GSH peroxidase in protecting the membrane of rat liver mitochondria. Biochim Biophys Ada, 113: 210-213.
Hancock, J.L. 1951. A staining technique for the study of temperature shock in semen. Nature, 167: 323-326.
Koonjanek, S., Pongpeng, P., Wirojwuthikul, S., Johannisson, A., Kunavongkrit, A. and Rodriguez- Martinez, H. 2007. Seasonality affects post-thaw plasma membrane intactness and sperm velocities in spermatozoa from Thai AI swamp buffaloes (Bubalus bubalis). Theriogenology, 67: 1424.
Kosower, N.S., Marikovsky, Y., Wertheim and Danon, D. 1971 Glutathione oxidation and biophysical aspects of injury to human erythrocytes. Lab clin Med 78: 533-545.
Martı, E., Mara, L., Martı´, J.I., Muin˜o-Blanco, T. and Cebria´n-Pe´rez, J.A. 2007. Seasonal varia tions in antioxidant enzyme activity in ram seminal plasma. Theriogenology, 67:1446-1454. Mathevon, M., Buhr, M.M. and Dekkers, J.C. 1998. Environmental, management and genetic factors affecting semen production in Holstein bulls. Journal of Dairy Science, 81: 3321-3330. Okamura, N., Iwaki, Y., Hiramoto, S., Tamba, M., Bannai, S., Sugita, Y., Syntin, P., Dacheux, F. and Dacheux, J.L. 1997. Molecular cloning and characterization of the epididymis specific glutathione peroxidase-like protein secreted in the porcine epididymal fluid. Biochimica et Biophysica Acta, 1336: 99-109.
Setchell,B.R., D’Occhio, M.J., Hall, M., Laurie, M.S., Tucker, M. and Zupp, J.L. 1988. Is embryonic mortality increased in normal female rats mated to sub-fertile males? Journal of Reproduction and Fertility, 82: 567-574.
Sinha, M.P., Sinha, A.K., Singh,B.K. and Prasad,R.L.1996.Effect of glutathione on motility, enzyme leakage and fertility of frozen goat semen. Animal Reproduction Science, 41: 237-243. Slaweta, R. and Laskowaska, T. 1987. The effect of glutathione on the motility and fertility of frozen bull semen. Animal Reproduction Science, 13: 249-253.
Storey, B.T. 1997. Biochemistry of the induction and prevention of lipoperoxidative damage in human spermatozoa. Molecular Human Reproduction, 3: 203-213.
Valeanu, S., Johannisson, A., Lundeheim, N. and Morrell, J.M. 2015. Seasonal variation in sperm quality parameters in Swedish red dairy bulls used for artificial insemination. Livestock science, 173: 111-118.
Wildeus, S. and Entwistle, K.W. 1983. Spermiogram and sperm reserves in hybrid Bos indicus - Bos taurus bulls after scrotal insulation. Journal of Reproduction and Fertility, 69:711–716.
Downloads
Published
Issue
Section
How to Cite
