Haemato-Biochemical Profile And Immunity To 12th Passaged Eimeria Spp. Oocysts In Fowl (Gallus Domesticus)

Birendra Kishor; M A Quadri

doi:10.48165/

Authors

Birendra Kishor Department of Veterinary Parasitology, College of Veterinary Science and Animal Husbandry
M A Quadri International Institute of Veterinary Education and Research (IIVER), NH-10 Bahu Akbarpur, Rohtak - 124 001, Haryana (India)

DOI:

https://doi.org/10.48165/

Keywords:

Fowl, Eimeria spp, Hemato-biochemical, attenuated strain, immunity

Abstract

The present study was conducted in 24 fowls (Gallus domesticus) randomly divided into 3 groups. Group I was kept as negative control. Group II, positive control, was induced with 5000 field strain of sporulated Eimeria spp. oocysts and group III induced with 5000 passages strain of Eimeria spp. oocysts. On day 21, group II and III were challenged with homologous virulent strain of Eimeria spp. Blood and faecal samples were collected at days 26, 31 and 36. One bird from each group was sacrificed on day 28 for lesion scores. All haematological parameter differed significantly, except basophils. Serum transaminases, glucose, body weight and oocyts g-1 showed significant change. Heavy lesion score appeared in group II. High stimulation index value was found on day 31 and distinct band of precipitation was observed in group III. The passaged oocysts feeding might be useful in protecting mixed Eimeria species infection in fowls.

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References

Crouch, C.F., Andrews, S.J., Ward, R.G. and Francis, M.J. 2003. Protective efficacy of a live attenuated anti-coccidial vaccine administered to 1-day-old chickens. Avian Pathology, 32: 297-304.

Devi, I.K., Devi, Y.G., Chanu, K.V., Ali, M.A., Tiwari, J.G., Prampha, M., Malsawmkima, D. and Patra, G. 2010. Transmission electron microscopy (TEM) study on the oocyst of Eimeria tenella. International Journal of Poultry Science, 11: 1050-1055.

Dong, H., Suo, X., Wang, M. and Teng, K. 2006. Characteristics of a line of Eimeria necatrix after 16 successive passages of oocysts collected after peak oocysts production. Journal of Parasitology, 92: 1229-1234.

Goodman, M., Wolfe, H.R. and Norton, S. I951. Precipitin production in chickens VI. Effect of varying concentrations of sodium chloride on precipitate formation. Journal of Immunology, 66: 225-236.

Hirani, N.D., Hasnani, J.J., Dhami, A.J. and Khanna, K. 2007. Haemato-biochemical profile of broilers affected with coccidiosis. Journal of Veterinary Parasitology, 21: 25-28. Johnson, J. and Reid, W.M. 1970. Anticoccidial drugs lesion scoring techniques in battery and floor pen experiments with chickens. Experimental Parasitology, 28: 30-36.

Jorgensen, W.K., Anderson, G.R., Jeston, P.J., Blight, G.W. and Molloy, J.B. 2006. Selection and characterisation of two attenuated vaccine lines of Eimeria tenella in Australia. Australian Veterinary Journal, 84(3): 89-94.

Lee, J.T., Broussard, C., Fitz-Coy, S., Burke, P., Eckert, N.H., Stevens, S.M., Anderson, P.N., Anderson, S.M. and Caldwell, D.J. 2009. Evaluation of live oocysts vaccination or salinomycin for control of field-strain Eimeria challenge in broilers on two different feeding programs. Journal of Applied Poultry Research, 18: 458-464.

Lillehoj, H.S. 1991. Cell-mediated immunity in parasitic and bacterial diseases. pp. 155-181. In: Avian Cellular Immunology (ed. J.M. Sharma). CRC Press, Boca Raton, Florida, USA. Lillehoj, H.S and Trout, J.M. 1993. Coccidia: A review of recent advances on immunity and vaccine development. Avian Pathology, 22: 3-31.

Lowry, O.H., Rosebrough, N.J., Farr, A.L. and Randall, R.I. 1951. Protein measurement with the folin phenol reagent. Journal of Biological Chemistry, 193: 265-275.

Mc-Donald, V. and Shirley, M.W. 2009. Past and future: vaccination against Eimeria. Parasitology, 136: 1477-1489.

Miyamoto, T., Min, W. and Lillehoj, H.S. 2002. Lymphocyte proliferation response during Eimeria tenella infection assessed by a new, reliable, nonradioactive colorimetric assay. Avian diseases, 46: 10-16.

Mondal, D.K., Chattopadhyay. S., Batabyal. S., Bera. A.K. and Bhattacharya D. 2011. Plasma biochemical indices at various stages of infection with a field isolate of Eimeria tenella in broiler chicken. Veterinary World, 4: 404-409.

Mosmann, T. 1983. Rapid colorimetric assay for cellular growth and survival: Application to proliferation and cytotoxic assay. Journal of Immunological Methods, 65: 55-63. Ouchterlony, O. 1953. Antigen-antibody in gels. IV: Types of reactions in co-ordinated systems of diffusion. Acta Pathologica Microbiologica Scandinavica, 32: 231-241.

Pangasa, A. and Singla, L.D. 2007. Effect of coccidiostats and immunomodulators on haematology of Eimeria tenella infected broilers. Indian Veterinary Journal, 84: 1131-1134.

Birendra Kishor and M.A. Quadri

Pangasa, A., Singla, L.D. and Ashuma 2007. Biochemical alterations in chicken during Eimeria tenella infection medicated with coccidiostats and immunomodulator. The Indian Journal for Field Veterinarians, 3(2): 06-10.

Patra, G., Ali, M.A., Chanu, K.V., Jonathan, L., Joy, L.K., Prava, M., Ravindran, R., Das, G. and Devi, L.I. 2010. PCR based diagnosis of Eimeria tenella infection in broiler chicken. International Journal of Poultry Science, 9: 813-818.

Pierce, A.E., Long, P.L. and Smith, C.H. I962. Immunity to Eimeria tenella in young fowls (Gallus domesticus). Immunology, 5: 129-152.

Rose, M.E. 1975. Infections with Eimeria maxima and Eimeria acervulina in fowl. Parasitology, 70: 263-271.

Saad, M.B., Bona, T.D.M.M., Serafini, C.A.V., Romani, F., Alvarez, J.M., Camargo, M.L. and Santin, E. 2009. Evaluating different vaccine routes against Coccidiosis. International Journal of Poultry Science, 8: 980-984.

Schalm, O.W., Jain, N.C. and Carrol, E.J. 1975. Veterinary Haematology (3rd edn.). Lea and Febiger Philadelphia, USA.

Singla, L.D. and Gupta. S.K. 2012. Advances in diagnosis of coccidiosis in poultry. pp 615-628. In: Veterinary Diagnostics: Current Trends (eds. R.P. Gupta, S.R. Garg, V. Nehra and D. Lather), Satish Serial Publishing House, Delhi, India.

Snedecor, G.W. and Cochran, W.G. 2004. Statistical Method (8th edn.), Oxford and IBH Publishing Co., New Delhi, India.

Stoll, N.R. 1930. Investigation on the control of hookworm disease. An effective method of counting hook worm eggs in the faeces. American Journal of Hygiene, 3: 59-60. Vermeulen, A.N. 1998. Progress in recombinant vaccine development against coccidiosis. A review and prospects into next century. International Journal of Parasitology, 28: 1121-1130. Weber, F.H., Genteman, K.C., Le, M.M.A. Sr, L.D.O and Evans, N.A. 2004. Immunization of broiler chicks by in ovo injection of infective stages of Eimeria. Poultry Science, 83: 392-399.