A Simple Method For Screening Gray Mold Of Castor  (Ricinus Communis L.) Under Artificial Conditions

Sujatha; T Parvathy; M A Raoof; Bewin Douglas

doi:10.48165/

Authors

Sujatha ICAR-Indian Institute of Oilseeds Research, Rajendranagar, Hyderabad 500 030, Telangana (India)
T Parvathy ICAR-Indian Institute of Oilseeds Research, Rajendranagar, Hyderabad 500 030, Telangana (India)
M A Raoof ICAR-Indian Institute of Oilseeds Research, Rajendranagar, Hyderabad 500 030, Telangana (India)
Bewin Douglas Tamilnadu Agricultural University, Coimbatore, Tamilnadu (India)

DOI:

https://doi.org/10.48165/

Keywords:

Amphobotrys ricini, Botryotinia ricini, castor, gene expression, gray mold disease, sporulation, susceptible

Abstract

Botryotinia ricini (Godfrey) Whetzel, the gray mold pathogen of castor, inflictsserious crop lossesin short periods. The presentstudywasaimedat to develop a season-independent screening technique for gray mold in castor. Conidia from in vitro-raised pure culture of the anamorph Amphobotrys ricini were sprayed on separated mature as well as immature capsules of 4 castor genotypes (DCS 9, RG 2787, RG 3309 and RG 3216 R), placed in glass petriplates and incubated at 22-24ºC for 7 days. Genotype DCS-9 was highly susceptible to the disease, while genotype RG 3216 R exhibited least susceptibility. Immature capsules were more susceptible to disease than mature capsules. RNA degradation occurred in treated samples 2 days after treatment and control gene (UBQ) expression was unaffected till 4 hr post inoculation. Thus a reproducible, lab-based, season-independent, screening technique using petri-plates and severed castor capsules was developed for differential gene expression analysis of candidate genes involved in early infection and elucidating host-pathogen interactions in castor gray mold.

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References

Anjani, K., Raoof, M.A., Reddy, PAV and Rao C.H. 2004. Sources of resistance to major castor (Ricinus communis L.) diseases. Plant Genetic Resources Newsletter, 137: 46-48. Blanco-Ulate, B., Morales-Cruz, A., Amrine, K.C.H., Labavitch, J.M., Powell, A.L.T. and Cantu, D. 2014. Genome-wide transcriptional profiling of Botrytis cinerea genes targeting plant cell wall during infections to different hosts. Frontiers in Plant Science, 5: 435.

Buchwald, N.F. 1949. Studies in the Scletoriniaceae: I. Taxonomy of the Sclerotiniaceae. Kongelige Veterinaer- og Landbohojskole, Aarsskrift, 32: 1-116.

Cantu, D., Vicente, A.R., Labavitch, J.M., Bennett, A.B. and Powell, A.L.T. 2008. Strangers in the matrix: Plant cell walls and pathogen susceptibility. Trends in Plant Science, 13: 610-617. Chagas, H.A., Basseto, M.A., Rosa, D.D., Zanotto, M.D. and Furtado, E.L. 2010. Diagramatic scale to assess gray mold (Amphobotrys ricini) in castor bean (Ricinus communis L.). Summa Phytopathologica, 36: 164-167.

Christensen, M.J., Falloon, R.E. and Skipp, R.A. 1988. A petri-plate technique for testing pathogenicity of fungi to seedlings and inducing fungal sporulation. Australasian Plant Pathology, 17: 45-47.

Dange, S.R.S., Desai, A.G and Patel, S.I. 2005. Diseases of castor. pp. 211-234. In: Diseases of Oilseed Crops (eds. G.S. Saharan, N. Mehta and M.S. Sangwan). Indus Publishing Co, New Delhi, India.

Govrin, E.M and Levin, A. 2000. The hypersensitive response facilitates plant infection by the necrotrophic pathogen Botrytis cinerea. Current Biology, 10: 751-757.

Hennebert, G.L. 1973. Botrytis and Botrytis-like genera. Persoonia, 7: 183-204.

Sujatha T. Parvathy et al.

Holz, G., Coertze, S. and Williamson, B. 2007. The ecology of Botrytis on plant surfaces. pp. 9-28. In: Botrytis: Biology, Pathology and Control (eds. Y. Elad, B. Williamson, P. Tudzynski and N. Delens). Springer, Dordrecht, Netherlands.

Kikot, G.E., Hours, R.A. and Alconada, T.M. 2008. Contribution of cell wall degrading enzymes to pathogenesis of Fusarium graminearum: A review. Journal of Basic Microbiology, 48: 1-11. Lalève, A., Gamet, S., Walker, A.S., Debieu, D., Toquin, V and Fillinger, S. 2014. Site-directed mutagenesis of the P225, N230 and H272 residues of succinate dehydrogenase subunit B from Botrytis cinerea highlights different roles in enzyme activity and inhibitor binding. Environmental Microbiology, 16: 2253-2266.

Lionetti, V., Raiola, A., Camardella, L., Giovane, A., Obel, N., Pauly, M., Favaron, F., Cervone, F. and Bellincampi, D. 2007. Overexpression of pectin methylesterase inhibitors in Arabidopsis restricts fungal infection by Botrytis cinerea. Plant Physiology, 143:1871-1880.

Orellana, R.G. and Thomas, C.A. 1962. Nature of predisposition of castor bean to Botrytis. I. Relation of leachable sugar and certain other biochemical constituents of the capsule to varietal susceptibility. Phytopathology, 52: 533-538.

Orlowska, E., Fiil, A., Kirk, H.G., Llorente, B. and Cvitanich, C. 2012. Differential gene induction in resistant and susceptible potato cultivars at early stages of infection by Phytophthora infestans. Plant Cell Reports, 31: 187-203.

Parvathy, S.T., Raoof, M.A and Prasad, R.D. 2013. In vitro sporulation of grey rot pathogen of castor and development of a screening method for Botrytis grey rot under artificial epiphytotic conditions. DOR Newsletter, 19: 8.000

Rao, M.S., Rama Rao, C.A., Srinivas, K., Pratibha, G, Vidya Sekhar, S.M., Sree Vani, G and Venkateswarlu, B. 2012. Intercropping for management of insect pests of castor, Ricinus communis, in the semi-arid tropics of India. Journal of Insect Science, 12: 14-16.

Shetty, N.P., Jogersen, H.J.L., Jensen, J.D., Collinge, D.B and Shetty, H.S. 2008. Roles of reactive oxygen species in interactions between plants and pathogens. European Journal of Plant Pathology, 121: 267-280.

Soares, D.J. 2012. Gray mold of castor: a review. pp. 219-240. In: Plant Pathology (ed. C.J.R. Cumagun). InTech, Rijecka. doi:10.5772/30806.

Sreenivas, P., Mamilla, V.R and Chandrasekhar, K. 2011. Development of biodiesel from castor oil. International Journal of Energy Science, 1: 192-197.