Potential Reserviours Of Whitefly Transmitted Begomo Virus Causing Leaf Curl Disease In Chilli
DOI:
https://doi.org/10.48165/Keywords:
Begomovirus, chilli, leaf curl, whitefly, weed hostsAbstract
A total of 19 weeds viz., Abutulon theopharasti, Achyranthus aspara, Ageratum conizoides, Cyperus esculentus, Malvestrum coromandelianum, Amaranthus viridis, Boerhavia diffusa, Calotropisgigantean, Cannabis sativa, Commelina benghalensis, Digera arvensis, Eclipta alba, Euphorbia hirta, Tinospora cordifolia, Ipomoea pestigridis, Parthenium hysterophorus, Phylanthus niruri, Physalis minima and Tribulus errestris, belonging to 12 families, were collected from chilli (Capsicum annum) fields in Punjab (India) with the aim to find out alternate hosts of Begomoviruses affecting drastically chilli crop. Of these, Eclipta alba, Ageratum conizoides, Amaranthus viridis, Ipomoea pestigridis, Abutulon theopharasti, Cannabis sativa and Commelina benghalensis showed typical leaf curl symptoms with vein clearing as predominant symptom exhibited by the above symptomatic weed species. Rest weeds did not possess any symptom. All the weed species were subjected to PCR and only Commelina benghalensis was found positive for Begomovirus with expected fragment size of ~575 bp of core protein region. Further, rolling circle amplification (RCA) technique was adopted to develop concatemer of circular DNA of virus. The RCA product when used as template in PCR showed amplification (~575 bp) for all the symptomatic and asymptomatic weed species, except Calotropis gigantean. The phylogenitic analysis revealed the presence of chilli leaf curl India virus, tomato leaf curl Jodepur virus and papaya leaf crumple virus in weed samples. Perusal of literature revealed that Commelina benghalensis aspara, Boerhavia diffuda, Cyperus esculentus, Tinospora cordifolia and Ipomoea pestigridis have not so far been reported as hosts of Begomoviruses, so this is the first report in this direction.
Downloads
References
Borah, B.K. and Dasgupta, I. 2012. Begomovirus research in India a critical appraisal and the way ahead. Journal Bioscan, 37: 791-806.
Brown, J.K., Fauquent, C.M., Briddon, R.W., Zerbini, M., Moriones, E. and Castilo, N.J. 2012. Family Geminiviridae. pp. 351-373. In: Virus Taxonomy: Classification and Nomenclature of Viruses – 9th Report of the International Committee on Taxonomy of Viruses (eds. A.M.Q. King, M.J. Adams, E.B. Carstens and E.J. Lefkowitz). Elsevier Academic Press, USA.
Chattopadhyay, B., Singh, A.K., Yadav, T., Fauquet, C.M., Sarin, N.B. and Chakraborty, S. 2008. Infectivity of the cloned components of a Begomovirus: DNA beta complex causing chilli leaf curl disease in India. Archives of Virology, 153: 533-539.
Cosata, A.S. and Bennet, C.W. 1950. Whitefly transmitted mosaic of Euphorbia prunifolia. Phytopathology, 40: 266-283.
Dereeper, A., Guignon, V., Blanc, G., Audic, S., Buffet, S., Chevenet, F., Dufayard, J.F., Guindon, S., Lefort, V., Lescot, M., Claverie, J.M. and Gascuel, O. 2008. Phylogeny.fr: robust phylogenetic analysis for the non-specialist. Nucleic Acids Research, 36: 465-469.
Fiallo, O.E., Navas, C.J. and Martinez, Z.Y. 2010. Two novel Begomoviruses belonging to different lineages infecting Rhinchosia minima. Archives of Virology, 155: 2053-2058. Gaikwad, A.K., Sharma, A., Cheema, D.S. 2010. Tomato leaf curl Palampur virus infecting calendula and marigold in Punjab. Indian journal of Virology, 21: A47.
Gilbertson, R.L., Hidayat, S.H., Martine, R.T., Leong, S.A., Faria, J.C., Morales, F. and Maxwell, D.P. 1991. Differentiation of bean infecting geminiviruses by nucleic acid hybridization probes and aspects of bean golden mosaic in Brazil. Plant Disease, 75: 336-342.
Gregorio, J., Bernal, A., Banuelos, H.B., Alpuchi, A.G. and Hernandez, C. 2010. Analysis of a new strain of Euphorbia mosaic virus with distinct replication specificity unveils a lineage of Begomoviruses with short Rep sequences in the DNA-B intergenic region. Virology, 7: 275.
Gupta, V.K., Sharma, R., Singh, S., Jindal, J. and Dilawari, V.K. 2010. Efficiency of Bemisia tabaci (Gennadius) populations from plant-hosts for acquisition and transmission of cotton leaf curl virus. Indian Journal of Biotechnology, 9: 271-275.
Haider, M.S., Tahir, M., Saeed, A.H., Shah, A.H., Rashid, N., Javed, M.A. and Iqbal, J. 2007. Vinca minor: another host of a tomato infecting Begomovirus in Pakistan. African Crop Science Conference Proceedings, 8: 905-907.
Kang, S.S., Athar, M. and Cheema, S.S. 2004. Additional hosts for cotton leaf curl Geminivirus. Journal of Research PAU, 41: 85-88.
Asif Ahmad et al.
Li, J. and Zhou, X.P. 2010. Molecular characterization and experimental host-range of two Begomoviruses infecting Clerodendrum cyrtophyllum in China. Virus Genes, 41: 250-259. Lodhi, M.A., Ye, G.N., Weeden, N.F. and Reisch, B.A. 1994. Simple and efficient method for DNA extraction from grapevine cultivars and Vitis species. Plant Molecular Biology Reporter, 12: 6-13.
Marcia, E.R. and Wayne, A.M. 1997. Genetic diversity among geminiviruses associated with weed species Sida spp, Myacroptilium lathyroides and Wissadula amplissima from Jamaica. Plant Disease, 81: 1251-1258.
Morales, F.J. 2010. Distribution and dissemination of Begomoviruses in Latin America and the Caribbean. Archives of Virology, 146: 415-441.
Radhakrishnan, G., Malathi, V.G. and Verma, A. 2004. Detection of DNA A and DNA β associated with cotton leaf curl and some other plant diseases caused by whitefly transmitted geminiviruses. Indian Phytopathology, 57: 53-60.
Reddy, R.V.C., Colvin, J., Muniyappa, V. and Seal, S. 2005. Diversity and distribution of Begomoviruses infecting tomato in India. Archives of Virology, 150: 845-867. Sastry, K.S.M. 1984. Strains of tomato leaf curl virus and its perpetuation under field conditions. Journal of Turkish Phytopathology, 13: 87-90.
Seal, S.E., VanderBosch, F. and Jeger, M.J. 2006. Factors influencing Begomovirus evolution and their increasing global significance: Implications for sustainable control.Critical Reviews in Plant Science, 25: 23-46.
Sharma, A. and Kang, S.S. 2009. Cucurbits: A new host for Begomovirus in Punjab. Plant Disease Research, 24: 51-53.
Sharma, P. and Rishi, N. 2007. Cotton leaf curl disease, an emerging whitefly transmissible Begomovirus comlex. Plant Viruses, 1: 127-134.
Shih, S.L., Tsai, W.S., Green, S.K. and Singh, D. 2007. First report of tomato leaf curl Joydebpur virus infecting chilli in India. Plant Pathology, 56: 341.
Singh, J.A.S., Sahimann, H.S. and Kapur, S.P. 1994. Studies on whitefly Bemisia tabaci transmitted cotton leaf curl virus disease in Punjab. Journal of Insect Science, 7: 194-198. Sivalingam, P.N., Padmalatha, K.V., Mandal, B., Monga, D., Ajemra, B.D. and Malathi, V.G. 2007. Detection of Begomoviruses by PCR in weeds and crop plants in and around cotton field infected with cotton leaf curl disease. Indian Phytopathology, 60: 360-361.
Sivalingam, P.N., Sumiya, K.V. and Malathi, V.G. 2011. Carrot as a new host for a Begomovirus: Yellow mosaic disease of carrot reported in India. New Disease Reporter, 23: 34. Van Regenmortel, M.H.V., Fauquet, C.M., Bishop, D.H.L., Carstens, E.B., Estes, M.K., Lemon, S.M., Maniloff, J., Mayo, M.A., McGeoch, D.J., Pringle, C.R. and Wickner, R.B. 2000. Virus Taxonomy: The Classification and Nomenclature of Viruses. The 7th Report of the International Committee on Taxonomy of Viruses. San Diego, Academic Press, USA. Wyatt, S.D. and Brown, J.K. 1996. Detection of geminiviruses in aqueous extracts by polymerase chain reaction. Phytopathology, 86: 1288-1293
Downloads
Published
Issue
Section
How to Cite
