Production Of Biomass, Total Saponin And Proline Under  Varied Nitrogen Concentration In Suspension Cultures Of  Gymnema Sylvestre R. Br

Deepika Emmanuel; N S Shekhawat

doi:10.48165/

Authors

Deepika Emmanuel Plant Biotechnology Unit, Department of Botany, New Campus, Jai Narain Vyas University, Jodhpur – 342 033, Rajasthan (India)
N S Shekhawat Plant Biotechnology Unit, Department of Botany, New Campus, Jai Narain Vyas University, Jodhpur – 342 033, Rajasthan (India)

DOI:

https://doi.org/10.48165/

Keywords:

Biomass, Saponin, Concentration, Suspension Cultures

Abstract

Plants generally have medicinal value based on the kind and concentration of secondary metabolites they possess. One of such medicinally important plant Gymnema sylvestre R.Br. (Aslepiadaceae), commonly called ‘gurmar’, has widely been used in ayurvedic and homoepathic systems of medicine. Traditionally, this plant is used for its anti-diabetic potential attributed to the saponin named as gymnemic acid (Min et al., 2007). The nitrogen concentration in medium greatly influences the amount of secondary metabolite produced (Nagella and Murthy, 2011) as it is required for the expression of specific proteins (Sugiharto and Sugiyama, 1992). The NH4+ to NO3- ratio influences the amount of biomass produced (Nagella and Murthy, 2011). Secondary metabolites are accumulated in cells in response to biotic and abiotic stress. Proline is one of the compounds accumulated in response to stress (Yaish, 2015). The present study aimed to analyze the influence of nitrogen on biomass and total saponin production in G. sylvestre, through a cost

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References

Bates, L.S., Waldern, R.P. and Teare, F.D. 1973. Rapid determination of free proline from water stress studies. Plant and Soil, 39: 205-207.

Cui, H.X., Murthy, H.N., Wu, C.H. and Paek, K.Y. 2010. Sucrose-induced osmotic stress affects biomass, metabolite and antioxidant levels in root suspension cultures of Hypericum perforatum L. Plant Cell Tissue and Organ Culture, 103: 7-14.

Hiai, S., Our, H. and Nakajima, T. 1976. Color reaction of some sapogenins and saponins with vanillin and sulphuric acid. Planta Medica, 29: 116-122.

Kim, O.T., Kim, M.Y., Hong, M.H., Ahn, J.C. and Huang, B. 2004. Stimulation of asiticoside accumulation in the whole plant cultures of Centella asiatica (L.) Urban by elicitors. Plant Cell Reports, 23: 339-344.

Makkar, H.P.S, Siddhuraju, P. and Becker, K. 2007. Plant Secondary Metabolites. Humana Press Inc., Totowa, New Jersey, USA.

Min, J.Y., Jung, H.Y., Kang, S.M., Kim, Y.D., Kang, Y.M., Park, D.J., Prasad, D.T. and Choi, M.S. 2007. Production of tropane alkaloids by small-scale bubble column bioreactor cultures of Scopolia parviflora adventitious roots. Bioresource Technology, 98: 1748-1753.

Murashige, T. and Skoog, F. 1962. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiologia Plantarum, 15: 473-497.

Nagella, P. and Murthy, H.N. 2011. Effects of macronutrients and nitrogen source on biomass accumulation and withanolide - A production from cell suspension cultures of Withania somnifera (L.) Dunal. Plant Cell Tissue and Organ Culture, 104: 119-124.

Shekhawat, M.S. and Shekhawat, N.S. 2011. Microporpagation of Arnebia hispidissma (Lehm). DC. and production of alkanin from callus and cell suspension culture. Acta Physiologia Plantarum,33: 1445-1450.

Sugiharto, B. and Sugiyama, T. 1992. Effects of nitrate and ammonium on gene expression of phosphoenolpyruvate carboxylase and nitrogen metabolism in maize leaf tissue during recovery from nitrogen stress. Plant Physiology, 98: 1403-1408.

Wang, W.G., Li, R., Lin, B., Li, L., Wang, S.H. and Cahn, F. 2011. Effects of low nitrogen and drought stress on proline synthesis of Jatropha curcuas seedling. Acta Physiologia Plantarum. 33: 1591-1595.

Yaish, M.W. 2015. Proline accumulation is a general response to abiotic stress in the date palm tree (Phoenix dactylifera L.). Genetics and Molecular Research, 14: 9943-9950. Yu, K.W., Gao, W.Y., Hahn, E.J. and Paek, K.Y. 2001. Effects of macroelements and nitrogen sources on adventitious root growth and ginsenoside production in ginseng (Panax ginseng C.A. Meyer). Journal of Plant Biology, 44: 179-184.