In Vitro Propagation Of Chinar (Platanus Orientalis L.) Using Node  And Internode Explants

Wasia Showkat; S A Gangoo; Nayeema Jabeen; Nageena Nazir; Khurshid Hussain; Amjad M Husaini

doi:10.48165/

Authors

Wasia Showkat Division of Biotechnology,S.K. University of Agricultural Science and Technology of Kashmir, Shalimar, Srinagar - 190 025, Jammu & Kashmir (India)
S A Gangoo Faculty of Forestry, S.K. University of Agricultural Science and Technology of Kashmir, Shalimar, Srinagar - 190 025, Jammu & Kashmir (India)
Nayeema Jabeen Division of Vegetable Sciences,S.K. University of Agricultural Science and Technology of Kashmir, Shalimar, Srinagar - 190 025, Jammu & Kashmir (India)
Nageena Nazir Division of Vegetable Sciences,S.K. University of Agricultural Science and Technology of Kashmir, Shalimar, Srinagar - 190 025, Jammu & Kashmir (India)
Khurshid Hussain Division of Vegetable Sciences,S.K. University of Agricultural Science and Technology of Kashmir, Shalimar, Srinagar - 190 025, Jammu & Kashmir (India)
Amjad M Husaini Division of Biotechnology,S.K. University of Agricultural Science and Technology of Kashmir, Shalimar, Srinagar - 190 025, Jammu & Kashmir (India)

DOI:

https://doi.org/10.48165/

Keywords:

Chinar, Platanus orientalis, tissue culture, micropropagation, in vitro propagation

Abstract

The aim of present study was to standardize a micropropagation protocol for Chinar (Platanus orientalis L.) using nodes and internode segments as explant materials. The sterilization treatment with 0.1% mercuric chloride for 10 min showed highest survival percentage in nodal explants (40.2) and internodal explants (51.9). Internodal explants survivedsterilant treatment better than nodal explants. For shoot regeneration, MS (Murashige and Skoog medium) medium supplemented with indole butyric acid (IBA) (0.25 mg L-1), benzyl adenine (BA) (4 mg L-1) proved best for both nodal and internodal explants. The maximum number of shoots (9.7 explant-1) greater shoot length (3.9 cm) was obtained from the internodal explants on MS medium supplemented with BA (4 mg L-1) + IBA (0.25 mg L-1). MS medium supplemented with IBA (1 mg L-1) and BA (2 mg L-1) showed 100% rooting percentage as well as highest number and length of roots (15.7 shoot-1, 3.7 cm, respectively). The internodalexplants showed better response than nodal explant. Rooted plantlets were then transferred to perforated plastic pots containing sterilized mixture of sand and soil (1:1) ratio, and grown in greenhouse. The survival percentage after 4 weeks in greenhouse was recorded as 62.3%.

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