Isolation And Characterization Of Native Fluorescent  Pseudomonas From Rice Fields Of Assam (India)

Pranamika Sharma; Lohit Ch Bora; Kamalakshi Devi; Sumita Acharjee; Polash Deb Nath

doi:10.48165/

Authors

Pranamika Sharma Department of Plant Pathology, Department of Agricultural Biotechnology, Assam Agricultural University, Jorhat-785 013, Assam (India)
Lohit Ch Bora Department of Plant Pathology, Department of Agricultural Biotechnology, Assam Agricultural University, Jorhat-785 013, Assam (India)
Kamalakshi Devi Department of Plant Pathology, Department of Agricultural Biotechnology, Assam Agricultural University, Jorhat-785 013, Assam (India)
Sumita Acharjee Department of Plant Pathology, Department of Agricultural Biotechnology, Assam Agricultural University, Jorhat-785 013, Assam (India)
Polash Deb Nath Department of Plant Pathology, Department of Agricultural Biotechnology, Assam Agricultural University, Jorhat-785 013, Assam (India)

DOI:

https://doi.org/10.48165/

Keywords:

PGPR, proteolytic activity, Pseudomonas, rhizosphere,, rice, siderophore

Abstract

In present study ten isolate of Pseudomonas, isolated from rice rhizosphere,haracterized on the basis of morpho-biochemical and molecular characteristics. Screening of their plant growth promoting activities viz., siderophore production, proteolytic activity, ammonia production, IAA production and HCN production revealed that the isolate PfAs1 was most promising one with highest siderophore production (6.18 cm dia. of yellow halo) and proteolytic activity (5.85 cm dia. halo zone). The results showed that none of the isolates possessed all the tested PGP activities. IAA and ammonia production was determined quantitatively for all the isolates. The IAA and ammonia production in tested Pf strains ranged from 15.66 to 48.58 and 179.05 to 394.72 mg L-1. The phylogenetic analysis using 16S rRNA gene sequences showed phylogenetic position of different fluorescent Pseudomonads. The results revealed that the isolated strains were Pseudomonas fluorescens and P. putida.

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