Statistical Optimization Of Cultural Conditions For  Manganese Peroxidase Production By Coriolus Versicolor Mtcc 138 From Digested Cattle Dung Slurry

Rekha Sharma; Rouf Ahmad Dar; Ajit Kaur; Urmila Gupta Phutela

doi:10.48165/

Authors

Rekha Sharma Department of Microbiology,Punjab Agricultural University, Ludhiana – 141 004, Punjab (India)
Rouf Ahmad Dar Department of Microbiology,Punjab Agricultural University, Ludhiana – 141 004, Punjab (India)
Ajit Kaur Department of Microbiology,Punjab Agricultural University, Ludhiana – 141 004, Punjab (India)
Urmila Gupta Phutela School of Renewable Energy Engineering, Punjab Agricultural University, Ludhiana – 141 004, Punjab (India)

DOI:

https://doi.org/10.48165/

Keywords:

Coriolus versicolor MTCC 138, digested cattle dung slurry, manganese peroxidase, Placket-Burman model

Abstract

The present study reports the production of manganese peroxidase enzyme using digested cattle dung slurry as a substrate by Coriolus versicolor MTCC Digested cattle dung slurry was analyzed for proximate (total solids, volatile solids and pH), chemical (cellulose, hemicellulose, lignin and silica) and biochemical (total proteins and manganese peroxidase) composition. Manganese peroxidase production by C. versicolor MTCC 138, was optimized by a conventional ‘one variable at a time approach’ and then by Placket Burman design. Optimized fermentation conditions for the maximum manganese peroxidase (78.0 U mL-1) enzyme production were 25% slurry concentration, 5 days of incubation, temperature 25°C and inoculum size of 106 spores mL-1. This enzyme titre obtained by statistical optimization was 1.86- fold than that achieved by ‘one variable at a time approach’. It revealed that the effect of different cultural conditions (slurry concentration, spore concentration and incubation period) have statistically significant effect on enzyme production.

Downloads

Download data is not yet available.

References

Anonymous 2009. Workshop Report of All India Coordinated Research Project on Renewable Energy Sources. CIAE/RES/2009/2. Central Institute of Agricultural Engineering, Bhopal, India.

AOAC. 2000. Official Methods of Analysis. Association of Official Analytical Chemists, Maryland, USA.

Arantes, V., Silva, E.M. and Milagres, A.M.F. 2011. Optimal recovery process conditions for manganese peroxidase obtained by solid-state fermentation of eucalyptus residue using Lentinula edodes. Biomass and Bioenergy, 35: 4040-4044.

Arora, D.S. and Gill, P.K. 2000. Laccase production by some white rot fungi under different nutritional conditions. Bioresource Technology, 73: 283-285.

Bhatti, H.N. and Nawaz, S. 2009. Production of endoglucanase by Fusarium solani grown in solid state fermentation. Asian Journal of Chemistry, 21: 1943-1948.

Rekha Sharma et al.

Castillo, M.P., Ander, P. and Stenstroem, J. 1997. Lignin and manganese peroxidase activity in extracts from straw solid substrate fermentation. Biotechnology Techniques, 11: 701-706. Dar, R.A. and Phutela, U.G. 2015. Optimisation of thermostable laccase production by

Thermoascus aurantiacus MTCC 375 using paddy straw as substrate and digested biogas slurry (DBS) as medium in solid state fermentation. Journal of Biofuels and Bioenergy, 1: 192-207.

Drista, V. and Rigas, F. 2006. Influence of cultivation temperature on the ligninolytic activity of selected fungal strains. In: Proceedings of the 5th WSEAS International Conference on Environment, Ecosystems and Development. Nov., 20-22, 2006, Venice, Italy.

Elisashvili, V. and Kachlishvili, V. 2009. Physiological regulation of laccase and manganese peroxidase production by white-rot basidiomycetes. Journal of Biotechnology, 144: 37-42. Gianfreda, L., Rao, M.A. 2004. Potential of extra-cellular enzymes in remediation of polluted soils: A review. Enzyme and Microbial Technology, 35: 339-354.

Hiscox, J., Baldrian, P., Rogers, H. and Boddy, L. 2010. Changes in oxidative enzyme activity during interspecific mycelial interactions involving the white-rot fungus Trametes versicolor. Fungal Genetics and Biology, 47: 562-571.

Hofrichter, M. 2002. Review: Lignin conversion by manganese peroxidase (MnP). Enzyme and Microbial Technology, 30: 454-466.

Iqbal, H.M.N., Asgher, M. and Bhatti, H.N. 2011. Lignin degrading enzymes. Bioresources, 6: 1273-1287.

Kalogeris, E., Iniotaki, F., Topakas, E., Christakopoulos, P., Kekos, D. and Macris, B.J. 2003. Performance of an intermittent agitation rotating drumtype bioreactor for solid-state fermentation of wheat straw. Bioresource Technology, 86: 207-213.

Kim, J. H., Hosobuchi, M., Kishimoto, M., Seki, T. and Ryu. D.Y. 1985. Cellulase production by a solid state culture system. Biotechnology and Bioengineering, 27: 1445-1450. Kundu, A.B., Ghosh, B.S., Ghosh, B.L. and Ghose, S.N. 1983. Annual Report on Fermentation Process, 7: 213-356.

Levin, L., Forchiassin, F. and Viale, A. 2005. Ligninolytic enzyme production and dye decolorization by Trametes trogii: application of the Plackett-Burman experimental design to evaluate nutritional requirements. Process Biochemistry, 40: 1381-1387.

Lowry, O.H., Rosebrough, N.J., Farr, A.L. and Randall, R.J. 1951. Protein measurement with Folin –phenol reagent. Journal of Biological Chemistry, 193: 265-275.

Macedo, J.A., Sette, L.D. and Sato, H.H. 2008. Optimization studies for the production of microbial transglutaminase from a newly isolated strain of Streptomyces sp. Food Science and Biotechnology, 17: 904-911.

Martinez, T., Speranza, M., Ruiz-Duenas, F.J., Ferreira, P., Camarero, S., Guillen, F., et al. 2005. Biodegradation of lignocellulosics: microbial, chemical, and enzymatic aspects of the fungal attack of lignin. International Microbiology, 8: 195-204.

Paszczynski, A., Huynh, V.B. and Crawford, R. 1988. Enzymatic activities of an extracellular manganese-dependent peroxidase from Phanerochaete chryosporium. FEMS Microbiology Letters, 29: 37-41.

Patel, H., Gupte, A. and Gupte, S. 2009. Effect of different culture conditions and inducers on production of laccase by a basidiomycete fungal isolate Pleurotus ostreatus HP-1 under solid state fermentation. Bioresources, 4: 268-284.

Reddy, L.V.A., Wee, Y.J., Yun, J.S. and Ryu, H.W. 2008. Optimization of alkaline protease production by batch culture of Bacillus sp. RKY3 through Plackett-Burman and response surface methodological approaches. Bioresource Technology, 99: 2242-2249.

Regina, M., Broetto, F., Giovannozzi-Sermanni, G., Marabotini, R. and Peranni, C. 2008. Influence of stationary and bioreactor cultivation on Lentinula edodes (Berk) Pegler lignocellulytic activity. Brazilian Archives of Biology and Technology, 51: 223-233.

Optimization to enhance the production of manganese peroxidase 131

Sabu, A., Swati, C. and Pandey, A. 2006. Tannase production by Lactobacillus sp. ASR-S1 under solid-state fermentation. Process Biochemistry, 41: 575-580.

Shaheen, I., Bhatti, H.N. and Ashraf, T. 2008. Production, purification and thermal characterization of invertase from a newly isolated Fusarium sp. under solid state fermentation. International Journal of Food Science and Technology, 4: 1152-1158.

Tekere, M., Mswaka, A.Y., Zvauya, R. and Read, J.S. 2001.Growth, dye degradation and lignolytic activity studies on Zimbabwean white rot fungi. Enzyme and Microbial Technology, 28: 420- 426.

Tripathi, M.K., Mishra, A S., Vaithiyanathan. S., Prasad, R. and Jakhmola, R.C. 2008. Selection of white rot basidiomycetes for bioconversion of mustard (Brassica campestris) straw under SSF into energy substrate for rumen microorganism. Letters of Applied Microbiology, 46: 364-370.

Vivekanand, V., Dwivedi, P., Pareek, N. and Singh, R.P. 2011. Banana peel: A potential substrate for laccase production by Aspergillus fumigatus VkJ2.4.5 in solid-state fermentation. Applied Biochemistry and Biotechnology, 165: 204-220.

Young, R.A. and Akhtar, M. 1998. The need for new technologies in the pulp and paper industry: Chemical applications to pulp and paper processing. In: Environmentally Friendly Technologies for the Pulp and Paper Industry. (1st edn.), John Wiley & Sons, London, UK.

Zadrazil, F., Kamra, D.N., Isikhuemhen, O.S., Schuchardt, F. and Flachowsky, G. 1996. Bioconversion of lignocelluloses into ruminant feed with white rot fungi. Journal of Applied Animal Research, 10: 105-124.

Zhao, X., Huang, X., Yao, J., Zhou, Y. and Jia, R. 2015. Fungal growth and manganese peroxidase production in a deep tray solid-state bioreactor, and in vitro decolorization of poly R-478 by MnP. Journal of Microbiology and Biotechnology, 25: 803-813.