Assessment Of Genetic Diversity Among Chickpea  (Cicer Arietinum L.) Genotypes Using Seed Storage Protein  Profiling And Rapd Markers

H J Maisuria; R M Patel; K P Suthar

doi:10.48165/

Authors

H J Maisuria Department of Agriculture Biotechnology, Anand Agricultural University, Anand - 388 110, Gujarat (India)
R M Patel ASPEE SHAKILAM Biotechnology Institute, Navsari Agricultural University (NAU), Surat - 395 007, Gujarat (India)
K P Suthar Department of Plant Molecular Biology and Biotechnology, ASPEE College of Horticulture and Forestry, NAU, Navsari - 396 450 Gujarat (India)

DOI:

https://doi.org/10.48165/

Keywords:

Cicer arietinum, Fusarium wilt, genetic diversity, RAPD, SDS, PAGE, seed storage protein

Abstract

Seed storage protein profiling and RAPD were used in present study for the analysis of genetic diversity among 15 chickpea genotypes. SDS-PAGE of seed storage protein differentiated 15 genotypes into three major clusters. RAPD analysis was done using highly polymorphic ten primers which amplified 104 bands with 2.70 average polymorphic bands. RAPD analysis reported 24.4 average per cent polymorphism and highest in OPH-03. OPH-19 reported highly informative primer based on PIC and MI value. Similarity coefficients of RAPD data were ranged from 0.55 to 0.95. Dendrogram generated three major clusters which distinguished Fusarium wilt susceptible and resistant genotypes in cluster I and cluster II, respectively, whereas cluster III included both resistant and susceptible genotypes. RAPD marker was found more informative than seed storage protein profiling for diversity analysis. Further, no specific banding was detected that could discriminate resistant from susceptible genotypes critically.

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References

Aarif, M., Rastogi, N.K., Verulkar, S.B., Saxena, R.R., Xalxo, M.S., Chandrakar, P.K. and Johnson, P.L. 2015. Intraspecific diversity of Kabuli chickpea genotypes based on seed storage protein profiling. Journal of Food Legumes, 28: 31-33.

Abou-El-enain, M.M. and Ahmed, S.M. 2012. Intraspecific diversity of Egyptian and foreign new lines of chickpea based on sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS PAGE) and isozyme markers. African Journal of Biochemistry Research, 6(10):135-139.

Agrawal, P.K. and Srivastava, A. 2010. Assessment of genetic diversity among chickpea cultivars of India using RAPD markers. Indian Journal of Genetics and Plant Breeding, 70: 264-270. Ahmad, Z., Mumtaz, A.S., Ghafoor, A., Ali, A. and Nisar, M. 2014. Marker-assisted selection (MAS) for chickpea Fusarium oxysporum wilt resistant genotypes using PCR based molecular markers. Molecular Biology Reports, 41: 6755-6762.

Anonymous. 2015. FAO Statistical Databases. Food and Agriculture Organization of the United Nations, Rome, Italy (http://www.fao.org/faostat/en/#data/QC)

Barman, P. 2012. Study on Association of Molecular Markers with Fusarium Wilt Resistance in Chickpea (Cicer arietinum L.). Ph.D. Thesis, Department of Biotechnology. Gauhati University, Gauhati, Assam, India.

Bhagyawant, S.S., Gupta, N., Gautam, A., Chaturvedi, S.K. and Shrivastava, N. 2015. Molecular diversity assessment in chickpea through RAPD and ISSR Markers. World Journal of Agricultural Research, 3(6): 192-197.

Bradford, M.M. 1976. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Analytical biochemistry,72: 248-254. Chakraborty, D., Sarkar, A., Gupta, S. and Das, S. 2006. Small and large scale genomic DNA isolation protocol for chickpea (Cicer arietinum L.), suitable for the molecular marker and transgenic analyses. African Journal of Biotechnology, 5(8): 585-589.

Chaube, H.S. and Pundhir, V.S. 2009. Vascular disease. pp. 461-467. In: Crop Diseases and their Management (2nd edn.), PHI Learning Private Limited, New Delhi, India.

Chawla, H.S. 2012. Introduction to plant biotechnology. pp. 356-397. In: Molecular Marker and Marker Assisted Selection (3rd edn.). Oxford & IBH Publishing Co., New Delhi, India. Datta, J. and Lal, N. 2011. Characterization of genetic diversity in Cicer arietinum L. and Cajanus cajan L. Millspaugh using random amplified polymorphic DNA and simple sequence repeat markers. Genomics and Quantitative Genetics, 3: 30-41.

Devi, R., Nandeesha, P., Kaashyap, M., Kumara, S. and Datta, S. 2014. Phylogenetic analysis of pulse crops using RAPD and seed storage protein markers. Indian Journal of Agricultural Biochemistry, 27: 81-84.

Ghafoor, A., Ahmad, Z., Qureshi, A.S. and Bashir, M. 2002. Genetic relationship in Vigna mungo (L.) Hepper and V. radiata (L.) R. Wilczek based on morphological traits and SDS-PAGE. Euphytica, 123: 367-378.

Hameed, A., Shah, M.T., Atta, B.M., Iqbal, N., Haq, M.A. and Ali, H. 2009. Comparative seed storage protein profiling of Kabuli chickpea genotypes. Pakistan Journal of Botany, 41: 703- 710.

Haware, M.P. and Nene, Y.L. 1980. Influence of wilt at different stages on the yield loss in chickpea. Tropical Grain Legume Bulletin, 19: 38-40.

Iqbal, S.H., Ghafoor, A. and Ayub, N. 2005. Relationship between SDS-PAGE markers and Ascochyta blight in chickpea. Pakistan Journal of Botany, 37: 87-96.

Javid, A., Ghafoor, A. and Anwar, R. 2004. Seed storage protein electrophoresis in groundnut for evaluating genetic diversity. Pakistan Journal of Botany, 36: 87-96.

Laemmli, U.K. 1970. Cleavage of structure proteins assembly of the head of bacteriophage T4. Nature, 22: 680-685.

H.J. Maisuria et al.

Nisar, M., Ghafoor, A., Khan, M.R., Ahmad, H., Qureshi, A.S. and Ali, H. 2007. Genetic diversity and geographic relationship among local and exotic chickpea germplasm. Pakistan Journal of Botany, 39: 1575-1581.

Rasool, S. 2013. Genetic diversity as revealed by RAPD analysis among chickpea genotypes. Pakistan Journal of Botany, 45: 829-834.

Rohlf, F.J. 2004. Numerical Taxonomy and Multivariate Analysis System. Version 2.02i: 3-9. Exeter software, Applied Biostatics, New York, USA.

Singh, P.K., Panwar, N. and Bhagyawant, S.S. 2015. Evaluation of genotypic variation using SDS PAGE. European Journal of Biotechnology and Bioscience, 3(7): 39-40.

Sneath, P.H.A. and Sokal, R.R. 1973. The Principles and Practice of Numerical Classification. Numerical Taxonomy. W.F. Freeman & Co., San Francisco, USA.

Suthar, K.P., Bhatnagar, R., Shukla, Y.M., Patel, N.J., Suthar, V.P. and Patel, J.P. 2012. Assessment of genetic diversity among chickpea genotypes using RAPD, protein profiling and isozyme markers. Indian Journal of Agricultural Biochemistry, 25: 25-30.

Williams, S.J.K., Kubelik, A.R., Livak, K.J., Rafalski, J.A. and Tingey, S.V. 1990. DNA polymorphism amplified by arbitrary primers useful as a genetic maker. Nucleic Acids Research, 18: 6531-6535.

Winter, P., Benko-Iseppon, A.M., Huttel, B., Ratnaparkhe, M., Tullu, A., Sonnante, G., Pfa, V.T., Tekeoglu, M., Santra, D., Sant, V.J., Rajesh, P.N., Kahl, G. and Muehlbauer, F.J. 2000. A linkage map of the chickpea (Cicer arietinum L.) genome based on recombinant inbred lines from a C. arietinum and C. reticulatum cross: Localization of resistance genes for Fusarium wilt races 4 and 5. Theoretical and Applied Genetics, 101: 1155-1163.