Effect Of Enzymes, Temperature And Ph On Bacteriocin  Activity Of Bacillus Megaterium Against Some Fungal Pathogens

Djadouni Fatima

doi:10.48165/

Authors

Djadouni Fatima Department of Biology, Faculty of Natural Sciences, Mascara University, Mascara, Algeria

DOI:

https://doi.org/10.48165/

Keywords:

Anise, Bacillus megaterium, bacteriocin, characterization, spoilage fungi

Abstract

The members of genus Bacillus are known to produce a wide range of antimicrobial substances, including peptide and lipopeptide antibiotics and bacteriocins that show wide antimicrobial spectra against food-spoilage microorganisms. The present study was aimed toevaluate the efficacy of bacteriocins from Bacillus megaterium against some selected spoilage fungi. B. megaterium was isolated from anise (Pimpinella anisum). The spoilage fungi were isolated from banana, strawberry, orange, lemon, apple, anion, cucumber, carrot, turnip and corn. Antifungal activity of extracted bactreriocin was determined at wide pH range (2-10), storage temperatures (-18, 4, 30 and 45°C for 15 days) and enzymes treatment (trypsin, pepsin, amylase and lipase) by agar well diffusion method against nine fungi. Aspergillus niger, Penicillium digitatum, Penicillium italicum and Fusarium oxysporum were found sensitive to bacteriocin and showed maximum zone of inhibition (12-16 mm). Partial characterization showed that the bactériocin was a glycolipoprotein, stable at 2-8 pH range, actives at all storage temperatures and was extracted in stationary phase of growth.

Downloads

Download data is not yet available.

References

Abriouel, H., Franz, C., Ben Omar, N. and Gálvez, A. 2011. Diversity and applications of Bacillus bacteriocins. FEMS Microbiology Reviews, 35: 201-232.

Albano, H., Todorov, S.D., Van reenen, C.A., Hogg, T. and Dicks, L.M. 2007. Characterization of two bacteriocins produced by Pediococcus acidilactici isolated from Alheira, a fermented sausage traditionally produced in Portugal. Journal of Food Microbiology, 116: 239-247.

Anurag, S., Dahiy N., Tewari, R.P. and Hoondal, G.S. 2006. Production of extracellular lipase by Bacillus megaterium AKG-1 in submerged fermentation. Indian Journal of Biotechnology, 5: 179-183.

Barrow, G.L. and Feltham, R.K.A. 1993. Cowan and Steel’s Manual for the Identification of Medical Bacteria (3rd edn.). Cambridge University, UK Press, Cambridge, UK.

Biedendieck, R., Beine, R.., Gamer, M., Jordan, E., Buchholz, K., Seibel, J., Dijkhuizen, L., Malten, M. and Jahn, D. 2007. Export of affinity tagged Lactobacillus reuteri levansucrase by Bacillus megaterium and synthesis of novel fructosyl oligosaccharides. Applied of Microbiology and Biotechnology, 74: 1062-1073.

Brusilow, W.S. and Nelson, D.L. 1981. Improved purification and some properties of megacin Cx, a bacteriocin produced by Bacillus megaterium. Journal of Biology and Chemistry, 256: 159- 164.

Djadouni, F. 2016. Microbial defense systems in foods and feeds. Journal of Biological Control, 30(3): 129-148.

Djadouni, F. and Madani, Z. 2016. Recent advances and beneficial roles of Bacillus megaterium in agricultures and otheirs fields. International Journal of Biology, Pharmacy and Applied Sciences, 5: 3160-3173.

Donoghue, H.D. 1977. Production of megacins C and Cx: Presumptive evidence of extra chromosomal control. Antimicrobial Agents and Chemotherapy, 11: 34-37.

Khalil, R., Djadouni, F., Elbahloul, Y. and Omar, S. 2009a. The influence of cultural and physical conditions on the antimicrobial activity of bacteriocin produced by a newly isolated Bacillus megaterium 22 strain. African Journal of Food Sciences, 3: 11-22.

Bacteriocin activity of Bacillus megaterium against fungal pathogens 93

Khalil, R.., Elbahloul, Y., Djadouni, F. and Omar, S. 2009b. Isolation and partial characterization of bacteriocin produced by a newly isolated Bacillus megaterium 19 strain. Pakistan Journal of Nutrition, 8: 242-250.

Léon, M., Yaryura, P.M., Montecchia, M.S., Hernández, A.I., Correa, O.S., Pucheu, N.L, 2009. Antifungal activity of selected indigenous pseudomonas and bacillus from the soybean rhizosphere. International Journal of Microbiology, 2009: 1-9.

Lisboa, M.P., Bonatto, D., Bizani, D., Henriques, J.A.P. and Brandelli, A. 2006. Characterization of a bacteriocin-like substance produced by Bacillus amyloliquefaciens isolated from the Brazilian Atlantic forest. International Microbiology, 9: 111-118.

Malten, M., Biedendieck, R., Gamer, M., Drews, A.C., Stammen, S., Buchholz, K., Dijkhuizen, L. and Jahn, D. 2006. A Bacillus megaterium plasmid system for the production, export and one step purification of affinity tagged heterologous levansucrase from growth medium. Applied and Environmental Microbiology, 72: 1677-1679.

Martirani, L., Varcamonti, M., Naclerio, G. and Maurilio, De Felice. 2002. Purification and partial characterization of bacillocin 490, a novel bacteriocin produced by a thermophilic strain of Bacillus licheniformis. Microbial Cell Factories. 1: 1-5.

Maulin, P.S. 2014. Bioremedial application of Bacillus Megaterium PMS82 in microbial degradation of acid orange dye. International Journal of Environmental Bioremediation and Biodegradation, 2(3): 93-99.

Ogunbanwo, S.T., Fadahunsi, I.F. and Molokwu, A.J. 2014. Thermal stability of lactic acid bacteria metabolites and its application in preservation of tomato pastes. Malaysian Journal of Microbiology, 10: 15-23.

Periago, P.M., Begoña Delgad, R.C., Fernández, P.S., Palop, A. 2006. B. megaterium spore germination and growth inhibition. Food Technology and Biotechnology, 44(1): 17-23. Radhakrishnan, R., Hashem, A. and Elsayed, F.A. 2017. Bacillus: A biological tool for crop

improvement through biomolecular changes in adverse environments. Frontiers in Physiology. 8(667): 1-14.

Rodrıguez Contreras, A., Koller, M., Miranda de Sousa Dias, M., Calafell Monfort, M., Braunegg, G. and Marques Calvo, M.S. 2013. High production of poly (3-hydroxybutyrate) from a wild Bacillus megaterium. Boliv. Str. Journal of Applied Microbiology, 114: 1378-1387.

Smaoui, S., Elleuch, L., Bejar, W., Karrai-Rebai, I., Ayadi, I, Jaouadi, B., Chouayekh, H., Bejar, S., Mellouli, L. and Mathieu, F. 2010. Inhibition of fungi and gram-negative bacteria by bacteriocin BacTN635 produced by Lactobacillus plantarum sp. TN635. Applied of Chemistry and Biotechnology, 162(4): 1132-1146.

Tournas, V.H. 2005. Spoilage of vegetable crops by bacteria and fungi and related health hazards. Critical Reviews in Microbiology, 31: 33-44.

Warth, A.D. 1978. Relationship between the heat resistance of spores and the optimum and maximum growth temperatures of Bacillus species. Journal of Bacteriology, 134: 699-705.