Agro-Biochemical Based Characterization And Molecular Profiling Of Linseed (Linum Usitattissimum L.) Cultivars

Authors

  • Chhaya Atri Department of Plant Breeding and Genetics, Punjab Agricultural University, Ludhiana - 141 004, Punjab (India)
  • Hitesh Kumar Department of Genetics and Plant Breeding, Banda University of Agriculture and Technology, Banda - 210 001, Uttar Pradesh (India)
  • Sanjula Sharma Department of Plant Breeding and Genetics, Punjab Agricultural University, Ludhiana - 141 004, Punjab (India)

DOI:

https://doi.org/10.48165/

Keywords:

Agro-biochemical characterization, fatty acid, fingerprinting, ISSR markers, linseed

Abstract

Nineteen commercial cultivars of flax (Linum usitatissimum L.) were subjected  to genetic diversity analysis, fingerprinting and identification of informative  marker allele(s) for seed purity assessment based on agro-morphological and biochemical quality traits and inter simple sequence repeat (ISSR) marker. A  total of 74 reproducible bands ranged from 120 to 1200 bp were amplified out  of which 68 were polymorphic among the cultivars. Dendrogram constructed  showed no clear grouping of cultivars with regard to the parentage/pedigree  record and breeding centres. The 2D plot obtained with PCA analysis clearly  supported the results obtained by cluster analysis. Further, ISSR profiling helped in the identification of 15 genotypes by cultivar specific bands. The  findings of this study would provide a useful guide for selecting specific  cultivar with a desirable agronomic trait with distinct genetic background for  linseed breeding program in India.  

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References

Alexander, D.E., Silela, S.L., Collina, F.I. and Rodgers, R.C. 1967. Analysis of oil content of maize by wide-line NMR. Journal of the American Chemical Society, 44: 555-558. AOAC. 2007. Official Methods of Analysis (18th edn). Association of Official Analytical Chemists, Arlington, USA.

Chhaya Atri et al.

Appelqvist, L.A. 1968. Rapid methods of lipid extraction and fatty acid methyl ester preparation for seed and leaf tissue with special remarks on preventing the accumulation of lipid contaminants. Arkiv For Kemi, Royal Swedish Academy of Science, 28: 551-570.

Bacchetta, G., Fenu, G., Mattana, E., Zecca, G., Grassi, F., Casazza, G. and Minuto, L. 2011. Genetic variability of the narrow endemic Rhamnus persicifolia Moris (Rhamnaceae) and its implications for conservation. Biochemical Systematics and Ecology, 39: 477-484.

Botstein, D., White, R.L., Skolnick, M. and Davis, R.W.1980. Construction of a genetic linkage map in man using restriction fragment length polymorphisms. The American Journal of Human Genetics, 32: 314-331.

Cloutier, S., Niu, Z., Datla, R. and Duguid, S. 2009. Development and analysis of EST-SSRs for flax (Linum usitatissimum L.). Theoretical and Applied Genetics,119:53-63.

Diederichsen, A. 2001. Comparison of genetic diversity of flax (Linum usitatissimum L.) between Canadian cultivars and a world collection. Plant breeding, 120: 360-362.

Diederichsen, A. and Fu, Y.B. 2006. Phenotypic and molecular (RAPD) differentiation of four infraspecific groups of cultivated flax (Linum usitatissimum L. subsp. usitatissimum). Genetic Resources and Crop Evolution, 53: 77-90.

Fu, Y.B. 2006. Genetics redundancy and distinctness of flax germplasm as revealed by RAPD dissimilarity. Plant Genetic Resources, 4: 117-124.

Hammer, Ø., Harper, D.A.T. and Ryan, P.D. 2001. PAST: Paleontological Statistics software package for education and data analysis. Palaeontologia Electronica, 4: 1-9. Iqbal, A., Nawaz, S., Babar, A.D., Bukhari, S.A., Amjad, M., Khan, M.A., Khan, S.A. and Ahmed, N. 2014. Estimation of genetic variability in linseed (Linum usitatissimum L.) using molecular marker: Molecular characterization of linseed genotypes. International Journal of Agriculture Sciences, 4: 151-156.

McDill, J., Repplinger, M., Simpson, B.B. and Kadereit, J.W. 2009. The phylogeny of Linum and Linaceae subfamily Linoideae, with implications for their systematics, biogeography and evolution of heterostyly. Systematic Botany, 34: 386-405.

Murray, M.G. and Thompson, W.F. 1980. Rapid isolation of high molecular weight plant DNA. Nucleic Acids Research, 8(19): 4321-4325.

Naresh, V., Yamini, K.N., Rajendrakumar, P. and Dinesh Kumar, V. 2009. EST-SSR marker-based assay for the genetic purity assessment of safflower hybrids. Euphytica, 170: 347-353. Nybom, H. and Weising, K. 2010. DNA-based identification of clonally propagated cultivars. Plant Breeding Review, 34: 221-295.

Nybom, H., Weising, K. and Rotter, B. 2014. DNA fingerprinting in botany: Past, present, future investigative. Genetics, 5: 1.

PAU. 2014. Package of Practices for Crops In Punjab: Rabi 2014. Punjab Agriculture University, Ludhiana, India.

Rajwade, A.V., Arora, R.S., Kadoo, N.Y., Harsulkar, A.M., Ghorpade, P.B. and Gupta, V.S. 2010. Relatedness of Indian flax genotypes (Linum usitatissimum L.): An inter-simple sequence repeat (ISSR) primer assay. Molecular Biotechnology, 45: 161-170.

Smykal, P., Bacova-Kerteszova, N., Kalendar, R., Corander, J., Schulman, A.H. and Pavelek, M. 2011. Genetic diversity of cultivated flax (Linum usitatissimum L.) germplasm assessed by retrotransposon-based markers. Theoretical and Applied Genetics, 122: 1385-1397.

Smykalova, I., Grillo, O., Bjelkova, M., Pavelek, M. and Venora, G. 2013. Phenotypic evaluation of flax seeds by image analysis. Industrial Crops and Products, 4: 232-238.

Sundaram, R.M., Naveenkumar, B., Biradar, S.K., Balachandran, S.M., Mishra, B., Ilyas, A.M., Viraktamath, B.C., Ramesha, M.S. and Sharma, N.P. 2008. Identification of informative SSR markers capable of distinguishing hybrid rice parental lines and their utilization in seed purity assessment. Euphytica, 163: 215-224.

Wiesnerova, D. and Wiesner, I. 2004. ISSR-based clustering of cultivated flax germplasm is statistically correlated to thousand seed mass. Molecular Biotechnology, 26: 207-214.

Agro-biochemical characterization and molecular profiling of linseed 145

Wu, M., Jia, X., Tian, L., and Lv, B. 2006. Rapid and reliable purity identification of F1 hybrids of

maize (Zea may L.) using SSR markers. Molecular Plant Breeding, 4(3): 381-384.

Yari, Z., Rahimlou, M., Poustchi, H. and Hekmatdoost, A. 2016. Flaxseed supplementation in

metabolic syndrome management: A pilot randomized, open-labeled, controlled study.

Phytotherapy Research, 30: 1339-1344.

Zexin, Jin. and Junmin, Li. 2007. Genetic differentiation in endangered Heptacodium miconioides

Rehd. based on ISSR polymorphism and implications for its conservation. Forest Ecology and

Management, 245: 130-136.

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Published

2018-06-03

Issue

Vol. 20 No. 2 (2018): Applied Biological Research (May) 2018

Section

Review Articles

How to Cite

Agro-Biochemical Based Characterization And Molecular Profiling Of Linseed (Linum Usitattissimum L.) Cultivars . (2018). Applied Biological Research, 20(2), 137–145. https://doi.org/10.48165/
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