Venturia Crataegi Causing Scab On Crataegus Songarica: Morpho Molecular Charcterization And A New Record From India

M S  Dar; Mushtaq Ahmad; M D Shah; A Padder; Nazir A Bhat; Anwar Khan; F A Ahanger; Asha Nabi; Aqleema Banoo

doi:10.48165/

Authors

M S Dar Division of Plant Pathology,Faculty of Agriculture, S.K. University of Agricultural Sciences & Technology of Kashmir (SKUAST-K), Wadura, Sopore - 192 301, Jammu & Kashmir (India)
Mushtaq Ahmad Directorate of Extension, SKUAST-K, Shalimar, Srinagar - 190 025, Jammu & Kashmir (India)
M D Shah Plant Virology and Molecular Plant Pathology Laboratory, Division of Plant Pathology, Faculty of Horticulture, SKUAST-K, Shalimar, Srinagar - 190 025, J&K (India)
A Padder Plant Virology and Molecular Plant Pathology Laboratory, Division of Plant Pathology, Faculty of Horticulture, SKUAST-K, Shalimar, Srinagar - 190 025, J&K (India)
Nazir A Bhat Mountain Field Crop Research Centre, SKUAST-K, Khudwani, Anantnag - 192 001, J&K (India)
Anwar Khan Division of Genetics and Plant Breeding, Faculty of Agriculture, S.K. University of Agricultural Sciences & Technology of Kashmir (SKUAST-K), Wadura, Sopore - 192 301, Jammu & Kashmir (India)
F A Ahanger Division of Plant Pathology,Faculty of Agriculture, S.K. University of Agricultural Sciences & Technology of Kashmir (SKUAST-K), Wadura, Sopore - 192 301, Jammu & Kashmir (India)
Asha Nabi Division of Plant Pathology,Faculty of Agriculture, S.K. University of Agricultural Sciences & Technology of Kashmir (SKUAST-K), Wadura, Sopore - 192 301, Jammu & Kashmir (India)
Aqleema Banoo Plant Virology and Molecular Plant Pathology Laboratory, Division of Plant Pathology, Faculty of Horticulture, SKUAST-K, Shalimar, Srinagar - 190 025, J&K (India)

DOI:

https://doi.org/10.48165/

Keywords:

Crataegus sonarica, phylogenetics, rDNA-ITS, scab, Venturia crataegi

Abstract

A field survey was conducted in the years 2017 to 2019 for the collection of diseased samples of various crops including Crataegusspecies, from Pulwama and Srinagar districts of Jammu and Kashmir. The leaf and fruit samples of Crataegus songarica showing scab symptoms were collected and brought to the laboratory. During disease monitoring it was noticed that the disease initiated as dark spots on leaves and fruits; and then often covered the entire surface. The mycelium on host was septate, immersed, subcuticular and pale olivaceous; hyphae branched and 2-4 µm wide.On MEA medium the mycelium was septate, branched, interwoven, light pale to olivaceous brown and 3-5 µm wide. The conidia were solitary, fusiform to obclavate having 0- 1 septum (avg. size; 15.64 × 5.76 µm). Based on morphocultural characteristics the fungus was identified as Venturia crataegi. The identity was confirmed by sequencing of ITS region of the pathogen using ITS1 and ITS4 primer. The ITS sequence was submitted to Genbank of NCBI under Accession No. MK478372. For phylogenetic analysis, the sequence was compared with other sequences of Venturia species available at NCBI which showed close relatedness of the present sequence with V. crataegi (Accession No. MH855705.1). The present study forms the first report of crataegus scab caused by Venturia crataegi from India.

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References

Beck, A., Ritschel, A., Schubert, K., Braun, U. and Triebel, D. 2005. Phylogenetic relationships of the anamorphic genus Fusicladium s. lat. as inferred by ITS nrDNA data. Mycological Progress, 4(2): 111-116.

Bowen, J.K., Mesarich, C.H., Bus, V.G.M., Beresford, R.M., Plummer, K.I.M.M. and Templeton, M.D. 2011. Venturia inaequalis: The causal agent of apple scab. Molecular Plant Pathology, 12: 105-122.

Braun, U. 2005. Proposal to conserve Fusicladium against Cycloconium (Hyphomycetes). Taxon, 54: 538.

Braun, U., Crous, P.W., Dugan, F., Groenewald, J.Z. and de Hoog, G.S. 2003. Phylogeny and taxonomy of Cladosporium-like hyphomycetes, including Davidiella gen. nov., the teleomorph of Cladosporium s. str. Mycological Progress, 2: 3-18.

Braun, U., Ritschel A. and Schubert, K. 2002. Proposal to conserve the generic name Fusicladium against Spilocaea (Hyphomycetes). Taxon, 51: 557.

Bruns, T.D., White, T.J. and Taylor, J.W. 1991. Fungal molecular systematics. Annual Review of Ecological Systematics, 22: 525-564.

Cesati, V. and De Notaris, G. 1863. Schema di classificazione deglisferiacei italici aschigeri. Commentario della Societa Crittog Italliano, 1: 177-240.

Dar, M.S., Irtefa, M., Sofi, T.A., Ahanger, F.A., Shah, M.D., Ahmad, M., Hamid, A., Mir, A.A., Nabi, A. and Padder, B.A. 2015. Distribution of apple scab race flora and identification of resistant sources against Venturia inaequalis in Kashmir. Plant Pathology Journal, 14(4): 196- 201.

De Notaris, G. 1844. Cenno sulla tribu de’pirenomiceti sferiacei edescrizione di alcuni nuovi generi. Giornale. Botanico Italliano, 1: 322-335.

Venturia crataegi causing scab on crataegus songarica 281

Faroouqi, K.D. and Dalal, M.A. 2003. Breeding apples for resistance to scab - An ecofriendly strategy. Acta Horticulture, 696: 33-37.

Gautam, A.K. 2014. Fusicladium ahmadii on Pyrus pashia: A new record for Indian mycobiota from Himachal Pradesh. Plant Pathology & Quarantine, 4(2): 86-89.

Kacho, N.F., Ahmed, K., Hussain, M., Bhat, M.A., Banday, S. and Nisar, Q. 2017. First record of scab disease of almond caused by Cladosporium carpophilum in India. Indian Phytopathology, 70: 403-404.

Kaul, A.K., Ghani, M.Y. and Kaul, R.N. 1989. A new scab disease of poplar from India. Plant Disease Research, 4(2): 187-188.

Liebhard, R., Koller, B., Patocchi, A., Kellerhals, M., Pfammatter, W., Jermini, M. and Gessler, C. 2003. Mapping quantitative field resistance against apple scab in a ‘Fiesta’ × ‘Discovery’ progeny, Genetics and Resistance, 93: 493-501.

Michalska, M.R. and Polec, E. 2006. The genus Fusicladium (Hyphomycetes) in Poland. Acta Mycologica, 41: 285-298.

Murray, M.G. and Thompson, W.F. 1980. Rapid isolation of high molecular weight plant DNA. Nucleic Acids Research, 8: 4321-4325.

Nath, P. 1935. Studies in the diseases of apples in Northern India II. A short note on apple scab due to Fusicladium dendritium Fuckel. Journal of Indian Botanical Society, 14: 121-124. Padder, B.A., Sofi, T.A., Ahmad, M., Shah, M.D., Hamid, A., Saleem, S. and Ahanger, F.A. 2013. Virulence and molecular diversity of Venturia inaequalis in commercial apple growing regions in Kashmir. Journal of Phytopathology, 161: 271-279.

Posada, D. and Buckley, T.R. 2004. Model selection and model averaging in phylogenetics: Advantages of Akaike information criterion and Bayesian approaches over likelihood ratio tests. Systematic Biology, 53: 793-808.

Putto, B.L. and Chaudhary, B.K.C. 1984. A study of pear scab in Kashmir - Symptomatology and morphology of the pear pathogen. Indian Phytopathology, 37: 699-701.

Rossman, A.Y., Crous, P.W., Hyde, K.D., Hawksworth D.L., Aptroot, A., Bezerra, J.L., Bhat, J.D., Boehm, E., Braun, U., Boonmees, S., Camporesi, E., Chomnunti, P., Dia, D.Q., D-souza, M.J., Dissanayake, A., Gareth-jones, E.B., Groenewald, J.Z., Harnandez-Restrepo, M., Hongsanan, S., Jaklitsch, W.M., Jayawardena, R., Jing, L.W., Krik, P.M., Lewery, J.D., Mopook, A., McKenzie, E.H., Monkai, J., Phillips, A.J., Phookamsak, R., Raja, H.A., Seifert, K.A., Senanayake, I., Slippers, B., Suetrong, S., Taylor, J.E., Thambugala, K.M., Tian, Q., Tibpromma, S., Wanasinghe, D.N., Wijayawardene, N.N., Wikee, S., Woundenberg, J.H., Wu, H.X., Yan, J., Yang, T. and Zhang, Y. 2015. Recommended names for pleomorphic genera in

Dothideomycetes. IMA Fungus, 6: 507-523.

Saccardo, P.A. 1882. Fungi Veneti novi vel critici vel Mycologiae Venetae addenti. Series VIII. Michelia, 1: 239-273.

Schnabel, G., Schnabel, E.L. and Jones, A.L. 1999.Characterization of ribosomal DNA from Venturia inaequalis and its phylogenetic relationship to rDNA from other tree-fruit Venturia species. Phytopathology, 89: 100-108.

Schubert, K., Ritschel, A. and Braun, U. 2003. A monograph of Fusicladium s. lat. (Hyphomycetes). Schlechtendalia, 9: 1-132.

Singh, S., Khan, S.N. and Mishra, B.M. 1983. Some new and noteworthy disease of poplar in India. Indian Forester, 109: 636-666.

Sivanesan, A. 1977. The taxonomy and pathology of Venturia species. Bibliotheca Mycologica, 59: 1-139.

Tamura, K., Peterson, D., Peterson, N., Stecher, G., Nei, M. and Kumar, S. 2013. MEGA v. 6.02: Molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance and maximum parsimony methods. Molecular Biology Evolution, 28: 2731-2739.

Valente, P., Ramos, J.P. and Leoncini, O. 1999. Sequencing as a tool in yeast molecular taxonomy. Canadian Journal of Microbiology, 45: 949-958.

M.S. Dar et al.

White, T.J., Bruns, T., Lee, S. and Taylor, J. 1990. Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics, pp. 315-322. In: PCR Protocol: A Guide to Methods and Applications (eds. M.A. Innis, D.H. Gelf, J.J. Sninsky and T.J. White). Academic Press, New York, USA.

Zhang, J., Zhou, Y., Dou. Z., He, W. and Zhang, Y. 2016. Type studies of six Venturia species. Nova Hedwigia, 102: 173-184.

Zhang, Y., Crous, P.W., Schoch, C.L., Bahkar, A.H., Guo L.D. and Hyde. K.D. 2011. A molecular, morphological and ecological re-appraisal of Venturiales - A new order of Dothideomycetes. Fungal Diversity, 51: 249-277.

Zhao, P., Kakishima, M., Uzuhashi, S. and Ishii, H. 2012. Multigene phylogenetic analysis of inter and intra-specific relationships in Venturia nashicola and Venturia pyrina. European Journal of Plant Pathology, 132: 245-258.