Antioxidant Enzyme And Biochemical Behaviour Of Chickpea Cv. Bg-256 And Csg-8962 Under Salt Stress Conditions

Authors

  • Swati Shahi Plant Physiology and Biochemistry Laboratory, Department of Botany, D.D.U. Gorakhpur University, Gorakhpur – 273 009, Uttar Pradesh (India)
  • Rajnish Kumar Plant Physiology and Biochemistry Laboratory, Department of Botany, D.D.U. Gorakhpur University, Gorakhpur – 273 009, Uttar Pradesh (India)
  • Malvika Srivastava Plant Physiology and Biochemistry Laboratory, Department of Botany, D.D.U. Gorakhpur University, Gorakhpur – 273 009, Uttar Pradesh (India)

DOI:

https://doi.org/10.48165/

Keywords:

Antioxidant enzymes, biochemical parameters, chickpea, Cicer arietinum, salinity

Abstract

A pot experiment was conducted to study antioxidant enzyme and  biochemical behaviour of two cultivars of chickpea (Cicer arietinum L.) viz.,  BG-256 (salt sensitive) and CSG-8962 (salt tolerant)] under salt-stress conditions. Salt stress was imposed by applying 50, 100 and 150 mM NaCl to plants as per treatment. Untreated plants were maintained as control.  Plant samples were analyzed from 20 DAS upto 60 DAS at 10-days interval.  Plant height and fresh weight decreased with increasing NaCl treatment.  The antioxidant enzymatic activities (superoxide dismutase, peroxidase and  catalase) and reducing sugar content were stimulated by salt stress. Enhancement was more in tolerant than sensitive cultivar. All the fractions  of photosynthetic pigments and carotenoid content in test plant decreased  gradually with increase in salt level. Decrease was more pronounced in  sensitive cultivar. 

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References

Abreu, I.A., Farinha, A.P., Negrão, S., Gonçalves, N., Fonseca, C., Rodrigues, M., Batista, R., Saibo, N.J. and Oliveira, M.M. 2013. Coping with abiotic stress: Proteome changes for crop improvement. Journal of Proteomics, 93: 145-168.

Amirjani, M.R. 2011. Effect of salinity stress on growth, sugar content, pigments and enzyme activity of rice. International Journal of Botany, 7: 73-81.

Arnon, D.I. 1949. Copper enzymes in isolated chloroplasts. Plant Physiology, 24: 1-15. Arefian, M., Vessal, S. and Bagheri, A. 2014. Biochemical changes in response to salinity in chickpea (Cicer arietinum L.) during early stages of seedling growth. Journal of Animal and Plant Sciences, 24: 1849-1857.Bashandy, T. and El-Shaieny, A.A.H. 2016. Screening of cowpea (Vigna unguiculata L. Walp) genotypes for salinity tolerance using field evaluation and molecular analysis. Journal of Agriculture Chemistry and Biotechnology, 7(9): 249-255. Basu, P.S., Berger, J.D., Turner, N.C., Chaturvedi, S.K., Ali, M. and Siddique, K.H.M. 2007. Osmotic adjustment of chickpea (Cicer arietinum) is not associated with changes in carbohydrate composition or leaf gas exchange under drought. Annals of Applied Biology, 150: 217-225.

Bergerson, F.J. 1980. Methods, accidents and design. pp. 3-10. In: Method for Evaluating Biological Nitrogen Fixation. (ed. F.J. Bergerson). John Wiley & Sons, New York, USA. Chance, B. and Maehly, A.C. 1955. Assay of catalase and peroxidase. Methods in Enzymology, 2: 764-775.

Chawla, S., Jain, S. and Jain, V. 2013. Salinity induced oxidative stress and antioxidant system in salt-tolerant and salt-sensitive cultivars of rice (Oryza sativa L.). Journal of Plant Biochemistry and Biotechnology, 22: 27-34.

El-Shaieny, A.A. 2015. Seed germination percentage and early seedling establishment of five (Vigna unguiculata L. Walp) genotypes under salt stress. European Journal of Experimental Biology, 5: 22-32.

El-Tayeb, M.A. 2005. Response of barley grains to the interactive effect of salinity and salicylic acid. Plant Growth Regulation, 45: 215-224.

FAO. 2011. FAOSTAT. Food and Agriculture Organization of the United Nations, Rome, Italy.

Swati Shahi et al.

Flowers, T.J., Gaur, P.M., Gowda, C.L.L., Krishnamurthy, L., Samineni, S., Siddique K.H.M., Turner, N.C., Vadez, V., Warshney, R.K. and Colmer, T.D. 2010. Salt sensitivity in chickpea. Plant Cell and Environment, 33: 490-509.

Gao, S., Ouyang, C., Wang, S., Xu, Y., Tang, L. and Chen, F. 2008. Effects of salt stress on growth, antioxidant enzyme and phenylalanine ammonia-lyase activities in Jatropha curcas L. seedlings. Plant Soil Environment, 54: 374-381.

Giannopolites, C.N. and Ries, S.K. 1977. Superoxide dismutase I. Occurrence in higher plants. Plant Physiology, 59: 309-314.

Gill, S.S. and Tuteja, N. 2010. Reactive oxygen species and antioxidant machinery in abiotic stress tolerance in crop plants. Plant Physiology and Biochemistry, 48: 909–930.

Hasan, M.K., Sabagh, A.E., Sikadar, M.S.I., Alam, M.J., Ratnasekera, D., Barutcular, C., Abdelaal, K.A.A. and Islam, M.S. 2017. Comparative adaptable agronomic traits of blackgram and mungbean for saline lands. Plant Archives, 17: 589-593

Hussain, K., Majeed, A., Nawaz, K., Bhatti, K.H. and Nisar, F.K. 2009. Effect of different levels of salinity on growth and ion contents of black seeds (Nigella sativa L.). Current Research Journal of Biological Sciences, 1: 135-138.

Kafi, M., Bagheri, A., Nabati, J., Zare M.M. and Masomi, A. 2011. Effect of salinity on some physiological variables of 11 chickpea genotypes under hydroponic conditions. Journal of Science and Technology of Green House Culture, 1(4): 55-70.

Kaur, P., Kaur, J., Kaur, S., Singh, S. and Singh, I. 2015. Salinity induced physiological and biochemical changes in chickpea (Cicer arietinum L.) genotypes. Journal of Applied and Natural Science, 6: 578-588.

Kibria, M.G., Hossain, M., Murata, Y. and Hoque, M.A. 2017. Antioxidant defense mechanisms of salinity tolerance in rice genotypes. Rice Science, [http://dx.doi.org/10.1016/j.rsci.2017.05.001].

Moud, A.M. and Maghsoudi, K. 2008. Application of coleoptile growth response method to differentiate osmoregulation capability of wheat (Triticum aestivum L.) cultivars. Research Journal of Agronomy, 2: 36-43.

Parvaiz, A. and Satyawati, S. 2008. Salt stress and phyto-biochemical responses of plants - A review. Plant Soil Environment, 54: 89-99.

Rasool, S., Ahmad, A. and Ahmad, P. 2013. Change in growth, lipid peroxidation and some key antioxidants enzymes in chick pea genotypes under salt stress. Acta Physiologiae Plantarum, 35. 1039-1050. 10.1007/s1738-012-1142-4.

Reddy, A.M., Shankhdhar, D. and Shankhdar, S.C. 2007. Physiological characterization of rice genotypes under periodic water stress. Indian Journal of Plant Physiology, 12: 189-193. Shahi, S. and Srivastava, M. 2016. foliar application of manganese for increasing salinity tolerance in mungbean. International Journal of Applied Biology and Pharmaceutical Technology, 7: 148-153.

Shannon, L.M., Kay, E. and Lew, J.Y. 1966. Peroxidase isoenzymes from horse radish roots. I. Isolation and physiological properties. Journal of Biological Chemistry, 241: 2166- 2172. Sheokand S., Dhandi, S. and Swaraj, K. 1995. Studies on nodule functioning and hydrogen

peroxide scavenging enzymes under salt stress in chickpea nodules. Journal of Plant Physiology, 33: 561-566.

Singh, S.L., Shahi, S. and Srivastava, M. 2017. Mitigation of salinity by different plant nutrients in Sesamum indicum (L.). New Agriculturist, 28: 49-55.

Somogyi, M. 1952. Notes on sugar determination. Journal of Biological Chemistry, 195: 19-23. Srivastava, M. and Shahi, S. 2018. Effect of salinity on morpho-physiological aspects, antioxidant enzymatic studies and yield attributes in wheat genotypes. Indian Journal of Plant Physiology, 23: 385-392.

Published

2019-03-01

How to Cite

Antioxidant Enzyme And Biochemical Behaviour Of Chickpea Cv. Bg-256 And Csg-8962 Under Salt Stress Conditions . (2019). Applied Biological Research, 21(1), 74–80. https://doi.org/10.48165/