Antioxidant Activities And Quantification Of Anthocyanins  In Some Coloured Grape Hybrids

Tanushree Sahoo; M K Verma; S K Singh; Madhubala Thakre; Shruti Sethi; Supradip Saha

doi:10.48165/

Authors

Tanushree Sahoo Division of Fruits and Horticultural Technology, ICAR-Indian Agricultural Research Institute, PUSA, New Delhi - 110 012 (India)
M K Verma Division of Fruits and Horticultural Technology, ICAR-Indian Agricultural Research Institute, PUSA, New Delhi - 110 012 (India)
S K Singh Division of Fruits and Horticultural Technology,ICAR-Indian Agricultural Research Institute, PUSA, New Delhi - 110 012 (India)
Madhubala Thakre Division of Fruits and Horticultural Technology,ICAR-Indian Agricultural Research Institute, PUSA, New Delhi - 110 012 (India)
Shruti Sethi Division of Food Science and Post-Harvest Technology, ICAR-Indian Agricultural Research Institute, PUSA, New Delhi - 110 012 (India)
Supradip Saha Division of Agricultural Chemicals, ICAR-Indian Agricultural Research Institute, PUSA, New Delhi - 110 012 (India)

DOI:

https://doi.org/10.48165/

Keywords:

Antioxidants, anthocyanins, FRAP, grape, HPLC

Abstract

Anthocyanins, the flavonoid group of polyphenols, is in great demand as an important food constituent, mainly due to its health promoting attributes. The present study was undertaken with 11 newly developed coloured grape hybrids at ICAR-IARI, New Delhi (India). Efforts were made to identify and quantify different anthocyanin fractions. HPLC chromatogram revealed ten different peaks belonging to anthocyanins, which varied significantly from 37.68 to 247.68 µg g-1in terms of C3G equivalent. The maximum peak height was at peak-5, which represents malvidin-3-glucoside. Maximum anthocyanin content was in grape hybrid ‘Hy.16/2A-R1 P18’. The anti oxidant activities of hybrids were measured by FRAP assay. The hybrid ‘Hy.16/2A-R1P19’ was found the most potent antioxidant rich genotype in terms of FRAP. The potential genotypes identified in terms of high bioactive contents can be helpful in protection against various chronic diseases.

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References

Benzie, I.F.F. and Strain, J.J. 1999. Ferric reducing (antioxidant) power as a measure of antioxidant capacity: the FRAP assay. Methods in Enzymology, 299: 15-36.

Clarke, R.J. and Bakker, J. 2004. Basic taste, stimulant and other constituents of wines. In: Wine Flavour Chemistry, Oxford: Blackwell Publishing Ltd., UK

Di Vittori Lucia, Mazzoni Luca, Battino Maurizio and Mezzetti Bruno. 2018. Pre-harvest factors influencing the quality of berries. Scientia Horticulture, 233: 310-322.

Duncan, D.B. 1955. Multiple range and multiple F tests. Biometrics, 11:1.

Garrido, J., Garrido, E.M. and Borges, F. 2011. Studies on the food additive propyl gallate: Synthesis, structural characterization, and evaluation of the antioxidant activity. Journal of Chemical Education, 89(1): 130-133.

Georgiev Vasil, Ananga Anthony and Tsolova Violeta. 2014. Recent advances and uses of grape flavonoids as Nutraceuticals. Nutrients, 6: 391-415.

Tanushree Sahoo et al.

Guidoni, S., Ferrandino, A. and Novello, V. 2008. Effects of seasonal and agronomical practices on skin anthocyanin profile of Nebbiolo grapes. American Journal of Enology and Viticulture, 59: 22-29. 15-17.

Guo, C., Yang, J., Wei, J., Li, Y., Xu, J., and Jiang, Y. 2003. Antioxidant activities of peel, pulp and seed fractions of common fruits as determined by FRAP assay. Journal of Nutritional Research, 23: 1719-1726.

Halvorsen, B.L., Carlsen, M.H., Phillips, K.M., Bohn, S.K., Holte, K., Jacobs, D.R. Jr. and Blomhoff, R. 2006. Content of redox-active compounds (antioxidants) in foods consumed in the United States. Journal of Clinical Nutrition, 84: 95-135.

He, J. and Giusti, M.M. 2010. Anthocyanins: Natural colorants with health-promoting properties. Annual Review of Food Science and Technology, 1: 163-187.

Jiang, M. and Zhang, J. 2001. Effect of abscisic acid on active oxygen species, antioxidative defence system and oxidative damage in leaves of maize seedlings. Journal of Plant Cell Physiology, 42: 1265-1273.

Kallithraka, S., Mohdaly, A.A.A., Makris, D.P. and Kefalas, P. 2005. Determination of major anthocyanin pigments in hellenic native grape varieties (Vitis vinifera spp.) and association with antiradical activity. Journal of Food Composition and Analysis, 18: 375-386.

Khoo Hock Eng, Azlan Azrina, Tang Sou Teng and Lim See Meng. 2017. Anthocyanidins and anthocyanins: Colored pigments as food, pharmaceutical ingredients, and the potential health benefits. Food and Nutrition Research, 61: 1-21

Liang, Z., Wu, B., Fan, P., Yang, C., Duan, W., Zheng, X. and Li, S. 2008. Anthocyanin composition and content in grape berry skin in Vitis germplasm. Food Chemistry, 111: 837- 844.

Meng, J.F., Fang, Y.L., Qin, M.Y., Zhuang, X.F. and Zhang, Z.W. 2012. Varietal differences among the phenolic profiles and antioxidant properties of four cultivars of spine grape (Vitis davidii Foex.) in Chongyi county (China). Food Chemistry, 134: 2049-2056.

Pazmiño-Durán, E.A., Giusti, M.M., Wrolstad, R.E. and Glória, M.B.A. 2001. Anthocyanins from banana bracts (Musa X paradisiaca) as potential food colorants. Food Chemistry, 73: 327- 332.

Pomar, F., Novo, M. and Masa, A. 2005. Varietal differences among the anthocyanin profiles of 50 red table grape cultivars studied by high performance liquid chromatography. Journal of Chromatography, 1094: 34-41.

Rockenbach, I.I., Gonzaga, L.V., Rizelio, V.M., Gonçalves, A.E.S.S., Genovese, M.I. and Fett, R. 2011. Phenolic compounds and antioxidant activity of seed and skin extracts of red grape (Vitis vinifera and Vitis labrusca). pomace from Brazilian winemaking. Food Research International, 44: 897-901.

Schaefer, H.M., Schaefer, V. and Levey, D.J. 2004. How plant–animal interactions signal new insights in communication. Trends in Ecology and Evolution, 19(11): 577-584. Takahama, U. 2004. Oxidation of vacuolar and apoplastic phenolic substrates by peroxidase: Physiological significance of the oxidation reactions. Phytochemistry Reviews, 3: 207-219. Tsuda, T. 2012. Dietary anthocyanin‐rich plants: Biochemical basis and recent progress in health benefits studies. Molecular Nutrition and Food Research, 56: 159-170.

Wrolstad, R.E., Durst, R.W. and Lee, J. 2005. Tracking color and pigment changes in anthocyanin products. Trends in Food Science and Technology, 16: 423-428.

Wrolstad, R.E. 2000. Anthocyanins. pp. 237-252. In: Natural Food Colorants (eds. F.J. Francis and G.J. Lauro). AVI: Marcel Dekker, New York. USA.

Wu, X. and Prior, R.L. 2005. Systematic identification and characterization of anthocyanins by HPLC–ESI–MS/MS in common foods in the United States: Fruits and berries. Journal of Agricultural and Food Chemistry, 53: 2589-2599.

Vermerris, W. and Nicholson, R. 2006. Phenolic Compound Biochemistry. Dordrecht: Springer, Dordrecht, Netherlands.