Genetic Enhancement Of Leaf Rust Resistance In Bread Wheat (Triticum Aestivum L.) Through Marker-Assisted Introgression Of Lr24 And Lr28
DOI:
https://doi.org/10.48165/Keywords:
Coefficient of incidence, marker-assisted introgression, rust resistance, SCAR markerAbstract
The present study was initiated with the objective of marker-assisted introgression of Lr24 and Lr28, wheat leaf rust resistance genes, into an elite susceptible bread wheat genotype MP3299 from NIL-PBW 343. Marker assisted selection alongwith screening of genotypes under epiphytotic conditions was undertaken in this study. SCAR molecular markers SCS73719 and SCS421570 linked to Lr24 and Lr28, respectively, confirmed in segregating population of MP3299 x NIL-PBW 343, were further evaluated for leaf rust resistance under artificial leaf rust condition. In F4 and F5 populations, high degree of leaf rust resistance was observed in plants carrying both Lr24 and Lr28 genes as compared to the plants with individual leaf rust resistance gene Lr24, followed by Lr28. Significant correlation and regression were observed between most of the agronomic traits and coefficient of incidence. Six Lr28 homozygous plants identified with high degree of leaf rust resistance were advanced for yield trials. The identified lines seem potential resistance sources and superior recombinants for future breeding programmes.
Downloads
References
Annapurnalilly, B. and Prashar, M. 2011. Validation and identification of molecular markers linked to the leaf rust resistance gene Lr28 in wheat. Journal of Applied Genetics, 52: 171-175. Arunkumar, G.S. 2013. Mechanism of Slow Leaf Ruster’s, Molecular Characterization in Bread
Wheat and Variability in Puccinia Triticina Eriks. Ph.D. thesis, University of Agricultural Sciences, Dharwad, Karnataka, India.
Bharadwaj, S.C., Parashar, M., Jain, S.K., Kumar, S., Sharma, Y.P., Sivasamy, M. and Kalappanavar, I. 2010. Virulence of Puccinia triticina on Lr28 and its evolutionary relation to prevalent pathotypes in India. Cereal Research Communication, 38: 83-89.
Deepak Koujalagi et al.
Bipinraj, A., Honrao, B., Prashar, M., Bhardwaj, S., Rao, S. and Tamhankar, S. 2010. Validation and identification of molecular markers linked to the leaf rust resistance gene Lr28 in wheat. Journal of Applied Genetics, 52: 171-175.
Bolton, M.D., Kolmer, J.A. and Garvin, D.F. 2008. Wheat leaf rust caused by Puccinia triticina. Molecular Plant Pathology, 9: 563-575.
Cherukuri, D.P., Gupta, S.K., Charpe, A., Koul, S., Prabhu, K.V., Singh, R.B. and Haq, Q.M.R. 2005. Molecular mapping of Aegilops speltoides derived leaf rust resistance gene Lr 28 in wheat. Euphytica, 143: 19-26.
Chhuneja, P., Kaur, S., Vikal, Y., Bains, N.S. and Dhaliwal, H.S. 2005. Development of PBW 343 near isogenic lines for leaf rust resistance genes Lr24 and Lr28. Crop Improvement, 32: 1-7. Chhuneja, P., Vikal, Y., Kaur, S., Singh, R., Junejam S., Bainsm N.S., Berry, O., Sharma, A., Gupta, S.K., Charpe, A., Prabhu, K.V. and Dhaliwal, H.S. 2011. Marker-assisted pyramiding of leaf rust resistance genes Lr24 and Lr28 in wheat (Triticum aestivum). Indian Journal Agriculture Sciences, 81: 214-218.
Dellaporta, S.L., Wood, J. and Hicks, J.B. 1983. A plant DNA mini-preparation: version II. Plant Molecular Biology Reporter, 1: 19-21.
Diaz, O.V., Kefauver, S.C., Elazab, A., Nieto-Taladriz, M.T. and Araus, J.A. 2015. Grain yield losses in yellow-rusted durum wheat estimated using digital and conventional parameters under field conditions. The Crop Journal, 3: 200-210.
El-Shamy, M.M., Minaas, A., Salam. and El-Kader, M.H. 2011. Effect of sowing density of some susceptible bread wheat cultivars on tolerance to leaf rust disease. Zagazig Journal Agriculture Research. 38(2): 339-352.
Hasan, M.A., Abu Aly, A.A. and El-Shehawy, A.E.A. 2012. Losses in wheat grain yield due to leaf rust, caused by puccinia triticina Eriks. Journal of Plant Protection and Pathology, 3: 959- 966.
Knott, D.R. 1989. The wheat rust breeding for resistance. Theory of Applied Genetics, 12: 63-69. Kolmer, J.A., Dyck, P.L. and Roelfs, A.P. 1991. An appraisal of stem and leaf rust resistance in North America hard red spring wheat and the probability of multiple mutations to virulence in population of cereal rust fungi. Phytopathology, 81: 237-239.
Kumar, A. and Raghavaiah, P. 2004. Effect of the leaf rust resistance gene Lr28 on grain yield and bread-making quality of wheat. Plant Breeding, 123: 35-38.
Dellaporta, S.L., Wood, J. and Hicks, J.B. 1983. A plant DNA mini-preparation: version II. Plant Molecular Biology Reporter, 1: 19-21.
Diaz, O.V., Kefauver, S.C., Elazab, A., Nieto-Taladriz, M.T. and Araus, J.A. 2015. Grain yield losses in yellow-rusted durum wheat estimated using digital and conventional parameters under field conditions. The Crop Journal, 3: 200-210.
McIntosh, R.A., Miller, T.E. and Chapman, V. 1982. Cytogenetical studies in wheat XII. Lr28 for resistance to Puccinia recondite and Sr34 for resistance to P. graminis tritici. Zeitschrift fur Pflanzenzuchtung, 89: 295-306.
McIntosh, R.A., Wellings, C.R. and Park, R.F. 1995. Wheat Rusts: An Atlas of Resistance Genes. CSIRO, East Melbourne, Australia.
McIntosh, R.A., Yamayaki, Y., Dubcovsky, J., Rogers, J., Morris, C. and Appels, R. (eds.) 2003. Catalogue of gene symbols for wheat. Proceedings Volume 4. In: 10th International Wheat Genetics Symposium. 1-6 September, 2003. Paestum, Italy.
Ochoa, J. and Parlevliet, J.E. 2007. Effect of partial resistance to barley leaf rust, Puccinia hordei, on the yield of three barley cultivars. Euphytica, 153: 309-312.
Peterson, R.F., Campbell, A.B. and Hanna. 1948. A diagrammatic scale for estimating rust severity on leaves and stems of cereals. Canadian Journal of Research, 26: 496-500. Prabhu, K.V., Gupta, S.K., Charpe, A. and Koul, S. 2004. SCAR marker tagged to the alien leaf rust resistance gene Lr19 uniquely marking the Agropyron elongatum derived gene Lr24 in wheat: A revision. Plant Breeding, 123: 417-420.
Leaf rust resistance through marker assisted introgression of Lr24 and Lr28 9
Revathi, P.S., Tomar, S.M.S., Vinod, S., Singh, N. 2010. Marker-assisted gene pyramiding of leaf rust resistance genes Lr24, Lr28 along with stripe rust resistance gene Yr15 in wheat (Triticum aestivum L.). Indian Journal of Genetics and Plant Breeding, 70: 349-354.
Roelfs, A.P. 1988. Resistance to leaf and stem rust. Chapter 2. In: Wheat Breeding Strategies for Resistance to the Rusts of Wheat. (eds. N.W. Simmonds and S. Rajaram), CIMMYT, Mexico. Samborski, D.J. and Dyck, P.L. 1982. Enhancement of resistance to Puccinia recondite by interaction
of resistance genes in wheat. Canadian Journal Plant Pathology, 4: 152-156. Sears, E.R. 1956. The transfer of leaf rust resistance from Aegilops umbellulata to wheat. pp. 1-22. In: Genetics in Plant Breeding. Brookhaven Symposia in Biology 9. Brookhaven National Laboratory, New York, USA.
Singh, R., Datta, D., Priyamvada, Singh, S. and Tiwari, R. 2004. Marker-assisted selection for leaf rust resistance genes Lr19 and Lr24 in wheat (Triticum aestivum L.). Journal of Applied Genetics, 45: 399-403.
Srivastava, C.P., Lal, S., Kushwaha, C., Chand, R., Singh, A.K., Singh, V.K. and Singh, B.D. 2009. Genetic variability in segregating generations and the breeding strategies to yield and resistance to rust in pea. Journal of Food Legumes, 22(3): 158-161.
Stakman, E.C., Stewart, D. and Loegering, W.Q. 1962. Identification of physiologic races of Puccinia graminis var. tritici. USDA Agriculture Research Service Bulletin, 15: 8-9.
Tiwari, S., Tomar, R.S., Chand, S. and Singh, N.K. 2014. Combining multiple rust resistant genes by phenotypic and marker assisted selection in wheat (Triticum aestivum L.). Indian Journal of Genetics, 74: 181-184.
Zhang, X.Y., Loyce, C., Meynard, J.M. and Monod, H. 2007. Modelling the effect of cultivar resistance on yield losses of winter wheat in natural multiple disease conditions. European Journal of Agronomy, 26: 384-393.
Downloads
Published
Issue
Section
How to Cite
