Isolation And Characterization Of Α–L-Rhamnosidase  Producing Bacterium, Agrococcus Sp. Bkd37, From A Warehouse  Soil And Partial Optimization Of Its Culture Conditions

Bhaba Kumar Pegu; Devid Kardong; Pankaj Chetia; Jitu Chutia; Dip Kumar Gogoi

doi:10.48165/

Authors

Bhaba Kumar Pegu Department of Life Sciences, Dibrugarh University, Dibrugarh – 786 004, Assam (India)
Devid Kardong Department of Life Sciences, Dibrugarh University, Dibrugarh – 786 004, Assam (India)
Pankaj Chetia Department of Life Sciences, Dibrugarh University, Dibrugarh – 786 004, Assam (India)
Jitu Chutia Department of Life Sciences, Dibrugarh University, Dibrugarh – 786 004, Assam (India)
Dip Kumar Gogoi Biotechnology Division, Central Muga Eri Research & Training Institute, Central Silk Board, Lahdoigarh, Jorhat – 785 700, Assam (India)

DOI:

https://doi.org/10.48165/

Keywords:

Agrococcus sp, α-L-rhamnosidase, bacterium, characterization, warehouse soil

Abstract

The present study was attempted to identify and partially optimize the culture conditions of a potential α-L-rhamnosidase producing bacterium isolated from warehouse soil of Dibrugarh, Assam (India). Twenty eight bacterial strains were isolated and the isolate BKD37 showed maximum rhamnosidase enzyme activity (10.01 ± 0.001 U mL-1) in crude culture medium. The 16S rRNA gene sequencing and phylogenetic analysis of BKD37 strain showed maximum similarity with that of Agrococcus sp. The culture conditions of strain BKD37 for the production of extracellular rhamnosidase enzyme was partially optimized in a basal medium in shake flask. The maximum rhamnosidase production was achieved after 60 h incubation at 30oC and pH 6.0. The rhamnose and NH4NO3 were found to e the best carbon and nitrogen sources, respectively, in basal medium for α-L rhamnosidase enzyme production.

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