Effect Of Dietary Supplementation Of L-Carnitine On Serum Lipid Profile And Antioxidant Status In Broiler Chicken Fed With Animal Fat-Rich Diet
DOI:
https://doi.org/10.48165/Keywords:
Broiler chicks, L-carnitine, reduced glutathione, serum cholesterol, superoxide dismutaseAbstract
A study was conducted to evaluate the effects of L-carnitine supplementation on serum lipid profile and antioxidant status of broilers fed with animal fat-based diet. Eighty days old straight run commercial broiler chicks (Vencobb) were randomly grouped into two treatment groups with four replications of ten chicks each. The rations were prepared with 5% animal fat. One group was supplemented with 900 mg L-carnitine kg-1 feed. The L-carnitine supplementation significantly reduced the serum cholesterol and LDL cholesterol levels; whereas serum triglyceride, HDL cholesterol and VLDL-cholesterol levels didn’t show any significant changes. The serum reduced-glutathione and superoxide dismutase concentrations were significantly higher in L-carnitine supplemented group. The study revealed that the supplementation of L carnitine not only improves the immune status but also decreases the serum cholesterol and LDL cholesterol level in broiler birds.
Downloads
References
AOAC. 2012. Official Methods of Analysis (19th edn.). Association of Official Analytical Chemists, Washington, USA.
Ardekani, H.M., Shevazad, M., Chamani, M., Aminafshar, M. and Arani, E.D. 2012. The effect of L-carnitine and low crude protein supplemented with crystalline essential amino acids diets on broiler chickens. Annals of Biological Research. 3: 1085-1093.
Arduini, A. 1992. Carnitine and its acyl esters as secondary antioxidants. American Heart Journal, 123: 1726–1727.
Augustyniak, A. and Skrzydlewska, E. 2009. L-carnitine in the lipid and protein protection against ethanol-induced oxidative stress. Alcohol, 43: 217-223.
BIS. 1973. Code for Handling, Processing, Quality Evaluation and Storage. IS: 7049-1973. Bureau of Indian Standards, New Delhi, India
BIS. 1992. Requirements for Chicken Feeds. IS: 1374-1992. Bureau of Indian Standards, New Delhi. India.
Cakir, S. and Yalcin, S. 2007. Effects of L-carnitine supplementation in diets with low or normal energy level on growth performance and carcass traits in broilers Revue de Medecine Veterinair, 158(6): 291-296.
Citil, M., Gunes, V., Atakisi, O., Ozcan, A., Tuzcu, M. and Dogan, A. 2005. Protective effect of L carnitine against oxidative damage caused by experimental chronic aflatoxicosis in quail. Acta Veterinaria Hungarica. 53: 319-324.
Corduk, M., Ceylan, N. and Ildiz, F. 2007. Effects of dietary energy density and L-carnitine supplementation on growth performance, carcass traits and blood parameters of broiler chickens. South African Journal of Animal Science, 37(2): 65-73.
Kalaiselvi, T. and Panneerselvam, C. 1998. Effect of L-carnitine on the status of lipid peroxidation and antioxidants in aging rats. Journal of Nutrition Biochemistry, 9: 575-581.
P. Murali et al.
Khoshkhoo, P.H., Azad, G.A., Ila, N., Moayer, F. and Nayeri, H.D. 2006. Effect of dietary L-carnitine supplementation on overall performance, carcass traits, serum components and immune response in broiler chicken. EPC 2006, paper 167. In: Proceedings of 12th European Poultry Conference. 10-14 September, 2006, Verona, Italy.
Koksal, B.H., Kucukersan, M.K. and Cakin, K. 2011. Effects of L-carnitine and/or inulin supplementation in energy depressed diets on growth performance, carcass traits, visceral organs and some blood biochemical parameters in broilers. Revue de Medecine Veterinaire, 11: 519-525.
Kuhajda, F.P. and Ronnett, G.V. 2007. Modulation of carnitine palmitoyltransferase-1 for the treatment of obesity. Current Opinion in Investigational Drugs, 8: 312-317. Kumaran, S., Savitha, S., Devi, M.A. and Panneerselvam, C. 2004. L-carnitine and DL-α-lipoic acid reverse the age-related deficit in glutathione redox state in skeletal muscle and heart tissues. Mechanisms of Ageing and Development, 125: 507-512.
Lawlor, J.B., Sheehan, E.M., Delahunty, C.M., Morrissey, P.A. and Kerry, J.P. 2003. Oxidative stability of cooked chicken breast burgers obtained from organic free range and conventionally reared animals. International Journal of Poultry Science, 2: 398-403.
Minami, M. and Yoshikawa, H.A. 1979. Simplified assay method of superoxide dismutase activity for clinical use. International Journal of Clinical Chemistry, 92: 337-342.
Moron, M.S., Depierre, J.W. and Mannervik, B. 1979. Levels of glutathione reductase and glutathione-S-transferase activities in rat lung and liver. Biochimica et Biophysica Acta, 582: 67-68.
Neuman, S.L., Lin, T.L. and Hester, P.Y. 2002. The effect of dietary carnitine on semen traits of White Leghorn roosters. Poultry Science, 81: 495-503.
NRC. 1994. Nutrient Requirements of Poultry (9th edn.). National Academy Press, Washington, USA. Parsaeimehr, K., Farhoomand, P. and Najafi, R. 2012. The effects of L-carnitine with animal fat on performance, carcass characteristics and some blood parameters of broiler chickens. Annals of Biological Research, 3(7): 3663-3666.
Resnik, A.Z., Kagan, V.E., Ramsey, R., Tsuchiya. M., Khwaja. S., Serbinova, E.A. and Packer, L. 1992. Antiradical effects of L-propionyl carnitine protection of the heart against ischemia reperfusion injury: The possible role of iron chelation. Archives of Biochemistry and Biophysics, 296: 394-401.
Snedecor, G.W. and Cochran, W.G. 1994. Statistical Methods. The Iowa State University Press, Ames, Iowa, USA.
SPSS. 16.0.2008. Statistical Procedures Companion. Prentice Hall Press, India. Tallentire, C.W., Leinonen, I. and Kyriazakis, I. 2018. Artificial selection for improved energy efficiency is reaching its limits in broiler chickens. Scientific Reports, 8: 1168. Thirumalaisamy, G., Muralidharan, J., Senthilkumar, S., Hema Sayee, R. and Priyadharsini, M. 2016. Cost-effective feeding of poultry. International Journal of Science, Environment and Technology, 6: 3997-4005.
Wang, J., Du, R., Qin, J., Wang, S., Wang, W., Li, H. and Pang, Q. 2003. Effect of yeast chromium and L-carnitine on lipid metabolism of broiler chickens. Asian-Australian Journal of Animal Science, 16(12):1809-1815.
Wang, Y.W., Ning, D., Peng, Y.Z. and Guo, Y.M. 2013. Effects of dietary L-carnitine supplementation on growth performance, organ weight, biochemical parameters and ascites susceptibility in broilers reared under low temperature environment. Asian-Australian Journal of Animal Science, 26: 233-240.
Zeyner, A. and Harmeyer, J. 1999. Metabolic functions of L‐carnitine and its effects as feed additive in horses. A review. Archives of Animal Nutrition, 52: 115-138.