Parental Polymorphism In Iron- And Zinc-Rich Rice  Varieties (Swarna And Type 3) Using Ssr Markers

D Shivani; K Suman; P Madhubabu; Ramya Rathod; C Cheralu; V  Gouri Shankar; C N Neeraja

doi:10.48165/

Authors

D Shivani Indian Institute of Rice Research, Rajendranagar, Hyderabad - 500 030, Telangana (India)
K Suman Indian Institute of Rice Research, Rajendranagar, Hyderabad - 500 030, Telangana (India)
P Madhubabu Indian Institute of Rice Research, Rajendranagar, Hyderabad - 500 030, Telangana (India)
Ramya Rathod Department of Genetics & Plant Breeding, College of Agriculture, Professor Jayashankar Telangana State Agricultural University, Rajendranagar, Hyderabad – 500 030, Telangana (India)
C Cheralu Department of Genetics & Plant Breeding, College of Agriculture, Professor Jayashankar Telangana State Agricultural University, Rajendranagar, Hyderabad – 500 030, Telangana (India)
V Gouri Shankar Department of Genetics & Plant Breeding, College of Agriculture, Professor Jayashankar Telangana State Agricultural University, Rajendranagar, Hyderabad – 500 030, Telangana (India)
C N Neeraja Indian Institute of Rice Research, Rajendranagar, Hyderabad - 500 030, Telangana (India)

DOI:

https://doi.org/10.48165/

Keywords:

Biofortification, iron, microsatellite markers, parental polymorphism, rice, zinc

Abstract

A microsatellite is a specific sequence of DNA bases which contains repeats. In present study, rice microsatellite (RM)markers were used to study the parental polymorphism between two parents. The experiment was conducted at Indian Institute of Rice Research, Rajendranagar Hyderabad (India). ‘Swarna’ and ‘Type 3’ rice varieties dense in grain iron and zinc were used to study the parental polymorphism. Out of 171 rice markers used for parental polymorphism, 52 markers showed polymorphism and 119 were monomorphic with expected base pair ranging from 84 to 383 bp. Therefore, these polymorphic rice microsatellite markers can be used in fine mapping of iron- and zinc-rich micronutrient genes to study the mapping populations of crosses obtained from these parents.

Downloads

Download data is not yet available.

References

Bouis, H.E. 2000. Enrichment of food staples through plant breeding: A new strategy for fighting micronutrient malnutrition. Nutrition, 16: 701-704.

Cakmak, I. 2002. Plant nutrition research: Priorities to meet human needs for food in sustainable ways. Plant and Soil, 247: 3-24.

Chen, X., Temnykh, S., Xu, Y., Cho, Y.G and McCouch, S.R. 1997. Development of a microsatellite framework map providing genome wide coverage in rice. Theoretical and Applied Genetics, 95: 553-567.

Cho, Y.G., Ishii, T., Temnykh, S. and Chen, X. 2000. Diversity of microsatellites derived from genomic libraries and. GenBank sequences in rice. Theoretical and Applied Genetics, 100: 713- 722.

Copenhagen Consensus. 2008. Press Release, Copenhagen Consensus Centre, Copenhagen Business. Gangaprasad, C., Ranjitkumar, N., Surapaneni, M., Deborah, D.A., Vipparla, A., Anuradha, G. and Vemireddy, L.R. 2013. Molecular genetic diversity of major Indian rice cultivars over decadal periods. PLoS ONE, 8: 66-197.

Kiranmayi, S.L., Manorama, K., Venkata, V.G.N.T., Radhika, K., Cheralu, C., Roja, V. and Sarla, N. 2014. Identification of markers associated with iron and zinc concentration in recombinant inbred lines of brown rice. Indian Journal of Genetics and Plant Breeding, 74: 423-429.

Phattarakul, N., Rerkasem, B., Li, L.J., Wu, L.H., Zou, C.Q., Ram, H., Sohu, V.S., Kang, B.S., Surek, H., Kalayci, M. and Yazici, A. 2012. Biofortification of rice grain with zinc through zinc fertilization in different countries. Plant and Soil, 361: 131-141.

Rahman, S.N., Islam, M.S., Alam, M.S. and Nasiruddin, K.M. 2007. Genetic polymorphism in rice (Oryza sativa L.) through RAPD analysis. Indian Journal of Biotechnology, 6: 224-229. Sambrook, J. 1989. Isolation of high-molecular-weight DNA from mammalian cells. Molecular Cloning, 44: 9-14.

Shankar, I. and Sarla, N. 2010. Microsatellite marker polymorphism in rice varieties rich in iron and zinc endosperm. Asian Journal of Experimental Biological Science, 1: 751-757. Stein, A.J. 2010. Global impacts of human mineral malnutrition. Plant and Soil, 335: 133-154. Wang, Y., Xue, Y. and Li, J. 2005. Towards molecular breeding and improvement of rice in China. Trends in Plant Science, 10: 610-614.

Welch, R.M. and Graham, R.D. 2004. Breeding for micronutrients in staple food crops from a human nutrition perspective. Journal of Experimental Botany, 55: 353-364.

White, P.J. and Broadley, M.R. 2009. Biofortification of crops with seven mineral elements often lacking in human diets – iron, zinc, copper, calcium, magnesium, selenium and iodine. New Phytologist, 182: 49-84.

Xu, Y. and Crouch, J.H. 2008. Marker-assisted selection in plant breeding: From publications to practice. Crop Science, 48: 391-407.