Phytal Proteins Of Jackfruit (Artocarpus Heterophyllus Lam.) And  Their Immuno-Compatibility Assessment With Indoor  Fungal Antigens And Human Antibody

Jayaprada Rao Chunduri; Mohak Prabhu

doi:10.48165/

Authors

Jayaprada Rao Chunduri Department of Biotechnology, Mithibai College of Arts, Chauhan Institute of Science and Amrutben Jivanlal College of Commerce and Economics, Vile Parle (West), Mumbai – 400 056, Maharashtra (India)
Mohak Prabhu Department of Biotechnology, Mithibai College of Arts, Chauhan Institute of Science and Amrutben Jivanlal College of Commerce and Economics, Vile Parle (West), Mumbai – 400 056, Maharashtra (India)

DOI:

https://doi.org/10.48165/

Keywords:

Allergens, jackfruit, immuno-binding, phytochemicals

Abstract

Several phytal extracts in Ayurveda are used in controlling certain allergies and bronchial disorders. In present study, the suspected anti-allergic activity of a tropical perennial tree, jackfruit (Artocarpus heterophyllus Lam), roots were evaluated against allergens that cause bronchial allergy/asthma. The chemical composition of phytal extracts indicated the presence of alkaloids, flavonoids, phytosterols, etc. with suspected anti-allergic capabilities. The binding capabilities between extracts, indoor fungal antigens and human antibodies (Ig E) based on different immunoassays indicated that jackfruit root protein of 75 kDa can be considered as possible and novel treatment option for common respiratory allergies.

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References

Abedin, M.S., Nuruddin, M.M., Hossain, A., Ahmed, K.U. and Hossain, A. 2012. Nutritive compositions of locally available jackfruit seeds (Artocarpus heterophyllus) in Bangladesh. International Journal of Bioscience, 2(8): 1-7.

Agarwal, S.S., and Singh, V.K. 1999. Immuno-modulators: A review of studies on Indian medicinal plants and synthetic peptides. PINSAB, 65: 377-392.

Ashok, K.R. and Ramaswamy, M. 2014. Phytochemical screening analysis by FT-IR spectroscopic analysis of selected Indian medicinal plants. International Journal of Current Microbiology and Applied Sciences. 3(1): 395-406.

Bello, M. and Falade, O.S. 2008. Studies on the chemical compositions and nutrients of some lesser known Nigeria fruits. African Journal of Biotechnology, 7: 3972-3979.

Bersabal, G., De Claro, C.G.D., Licatan, B.K.P. and Alaza, A.B. 2012. .Bronchodilating activity of formulated syrup of jackfruit (Artocarpus heterophyllus Moraceae) root extract in Albino rats. UIC Research Journal, 18: 247-262.

Chunduri, J.P. 2014. Indoor fungal populations inhabiting cement structures - Remedial measures. Journal of environmental Science, Toxicology and Food Science, 8(4): 9-24. Coates, J. 2000. Interpretation of infrared spectra, a practical approach. pp. 10815-10837. In: Encyclopedia of Analytical Chemistry. John Wiley, Chichester, UK.

Collins, C.H., Patricia, M.L., Grange, J.M. and Falkinham, J.O. 2004. Collins and Lyne’s Microbiological Methods (8th edn.). Arnold, London, UK.

Csako, G. 2019. Immunoelectrophoresis: A method with many faces. pp. 339-359. In: Electrophoretic Separation of Proteins. Methods in Molecular Biology, Vol. 1855 (eds. B. Kurien and R. Scofield). Humana Press, New York, USA.

Halilu, M.E., Abubakar, A., Garba, M.K. and Isah, A.A. 2012. Antimicrobial and preliminary phytochemical studies of methanol extract of root bark of Crossopteryx febrifuga (Rubiaceae) Journal of Applied Pharmaceutical Sciences, 2(12): 66-70.

Hornok, L. and Szecsi, A. 1977. Demonstration of antifungal antibodies in hyperimmune mouse ascitic fluid by means of counter immuno-electrophoresis. Journal of General Microbiology, 100: 213-216.

Jayaprada Rao Chunduri and Mohak Prabhu

Indumathi, K., Pushpa, N. and Anchana Devi, C. 2008. Application of counter immuno electrophoresis in the detection of antibodies to infectious bursal disease of poultry and its use in heterologous detections. Cauvery Research Journal, 2: 53-56.

Jagtap, U.B. and Bapat, V.A. 2010. Artocarpus: A review of its traditional uses, phytochemistry and pharmacology. Journal of Ethnopharmacology. 129: 142-166.

Kanwar, S. and Verma, M. 2006. Principles and applications of immuno-diffusion, immuno electrophoresis, immuno-fluorescence, ELISA, Western blotting, minimal inhibitory concentration, Kirby-Bauer method and Widal test. Immunology and Medical Microbiology (https://pdfs.semanticscholar.org/2a2d/84b822e75946f80e195b4ea41862e034df7c.pdf).

Manik, G., Barij, N.S., Julio, A. Seijas, M.P., Vázquez, T. and Xesús, F. 2014. Flavonoids and phenolic compounds from Litsea polyantha Juss. bark. In: 18th International Electronic Conference on Synthetic Organic Chemistry. 1-30 Nov., 2014. University of Santiago de Compostela, Spain. [http://www.sciforum.net/conference/ecsoc-18/].

Mayer, R.J. and Walker, J.H. 1987 Immunochemical Methods in Cell and Molecular Biology. Academic Press, London, UK.

Ozcan, T., Akpinar, B.A., Yilmaz, E.L. and Delikanli, B. 2014. Phenolics in human health. International Journal of Chemical Engineering and Application, 5: 393-396. Pravin, S., Diliprao, D., Nikita, D. and Jyoti, V. 2014. Preclinical evaluation and antiasthmatic acti vity of Euphorbia hirta L. International Journal of Pure & Applied Biosciences, 2: 262-266. Prem, J.V., Sajeshkumar, N.K., Jiby. J.M., Ajesh, C., Kuriakose, B.A., Renjith, J.M., Alen, N.A., Deenamol, T., Riny, S.T., Nijamol, V. and Sophyiamol, J. 2015. Chemistry and medicinal properties of jackfruit (Artocarpus heterophyllus): A review on current status of knowledge. International Journal of Innovative Research and Review, 3: 83-95.

Rao, J. 2013. Antioxidant and nutritional analysis of edible Cucurbitaceae vegetables of India. International Journal of Bioaasays, 2: 1128-1129.

Rosenberg, I.M. 2004. Protein Analysis and Purification Benchtop Technique (2nd edn). Birkhäuser, Boston, USA.

Sahu, N. and Saxena, J. 2013. Phytochemical analysis of Bougainvillea Glabra by FTIR and UV VIS spectroscopic analysis. International Journal of Pharmaceutical Sciences, 21: 196-198. Sawant, S.D. 2011. FT-IR spectroscopy: Principle, techniques and mathematics. International Journal of Pharma and Bioscience, 2: 514-519.

Saxena, P. and Saxena Priyanka 2014. In-vitro and in-vivo evaluation of anti-asthmatic activity of rhizome extracts of Acorus calamus in guinea pigs. Research Journal of Pharmaceutical Sciences, 3(5): 1-6.

Shrikant, B.S., Thakor, N.J., Haldankar, P.M. and Kalse, S.S.B. 2012. Jackfruit and its many functional components as related to human health: A reviews. Comprehensive Reviews in Food Science and Food Safety, 11: 565-576.

Starlin, T., Arul, R.C., Ragavendran, P. and Gopalakrishna, V.K. 2012. Phytochemical screening, functional groups and element analysis of Tylophora pauciflora Wight and Arn. International Research Journal of Pharmacy, 3: 180-183.

Ullman, D.E., German, T.L., Gunasinghe, U.B. and Ebesu, R.H. 1989. Serology of Closterovirus like particle associated with mealy bug wilt of pineapple. The American Phytopathological Society,79: 1341-1344.

Venkataraman, K. 1972. Wood phenolics in the chemotaxonomy of the Moraceae. Phytochemistry, 11: 1571-1586.

Valipour, N.R., Moulood, V.N. and Masoumeh, A. 2015. Determination of protein concentration using Bradford microplate protein quantification assay. International Electronic Journal of Medicine, 4: 11-17.

Yadav, M., Sanjukta, C., Sharad, K.G. and Geeta, W. 2014. Preliminary phytochemical screening of six medicinal plants used in traditional medicine. International Journal of Pharmacy and Pharmaceutical Sciences, 6: 539-542.