Detection Of Aflatoxin Producing Fungi In Rice And Peanut, And Determination Of Aflatoxin By Hplc-Fld Method
DOI:
https://doi.org/10.48165/Keywords:
Aflatoxin, HPLC-FLD, identification, peanut, riceAbstract
Peanut and rice are the widely consumed food material all over the globe. The contamination of these commodities with aflatoxin is a big challenge and a major health concern. In present study, two aflatoxin producing fungi were the isolated from rice and peanut and identified as Penicillium charlesii and Aspergillus candidus on the basis of morpho-molecular characteristics A sensitive, rapid and robust analytical method was developed and validated to determine the aflatoxin contamination level in aforementioned peanut and rice matrices. The method involved methanolic extraction of aflatoxin, immune affinity column clean-up followed by derivatisation with Kobra cell and fluorescence detection. The analysis of contaminated samples revealed only two peanut samples and one rice sample possessed aflatoxin B1 and traces of aflatoxin B2. The method showed 0.05 µg kg-1 as the limit of quantification which was below the maximum levels set by various global regulations. The method performance was in line with the performance criteria established by numerous regulatory bodies such as EU and SANTE.
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Ahmadi, B., Hashemi, S.J., Zaini, F., Shidfar, M.R., Moazeni, M., Mousavi, B., Noorbakhsh, F., Gheramishoar, M., Rezaie, S. and Rezaie, S. 2012. A case of onychomycosis caused by Aspergillus candidus. Medical Mycology Case Reports, 1(1): 45-48.
Ainiza, W.W., Jinap, S. and Sanny, M. 2015. Simultaneous determination of aflatoxins and ochratoxin A in single and mixed spices. Food Control, 50: 913-918.
Allwood, J.G., Wakeling, L.T., Post, L.S. and Bean, D.C. 2023. Food safety considerations in the production of traditional fermented products: Japanese rice Koji and Miso. Journal of Food Safety, 13048. [https://onlinelibrary.wiley.com/doi/pdfdirect/10.1111/jfs.13048].
Barnett, H.L. and Hunter, B.B. 1998. Illustrated Genera of Imperfect Fungi. American Phytopathological Society, Philadelphia, USA.
Bills, G.F. and Polishook, J.D. 1991. Microfungi from Carpinus caroliniana. Canadian Journal of Botany, 9: 1477-1482.
Caicedo, N.H., Davalos, A.F., Puente, P.A., Rodríguez, A.Y. and Caicedo, P.A. 2019. Antioxidant activity of exo‐metabolites produced by Fusarium oxysporum: An endophytic fungus isolated from leaves of Otoba gracilipes. Microbiology Open, 8(10): 903. [https://onlinelibrary.wiley.com/doi/pdfdirect/10.1002/mbo3.903].
Chen, Y.C., Liao, C.D., Lin, H.Y., Chiueh, L.C. and Shih, D.Y.C. 2013. Survey of aflatoxin contamination in peanut products in Taiwan from 1997 to 2011. Journal of Food and Drug Analysis, 21(3): 247-252.
Chayya, R.S., Membré, J.M., Nag, R. and Cummins, E. 2023. Farm-to-fork risk assessment of aflatoxin M1 in milk under climate change scenarios – A comparative study of France and Ireland. Food Control, 109713. [https://www.sciencedirect.com/science/article/pii/ S0956713523001135].
Codex Alimentarius. Codex Standard 193-1995. Available online: [http://www.fao.org/fileadmin/user_upload/agns/pdf/CXS_193e.pdf]. (accessed on 5Oct., 2022). Commission regulation (EC) No. 1881/2006. Official Journal of the European Union. L 364: 5-24. [https://eurlex.europa.eu/legalcontent/DE/AUTO/?uri=CELEX:02006R1881-20180319]. Dhanshetty, M. and Banerjee, K. 2019. Simultaneous direct analysis of aflatoxins and ochratoxin A in cereals and their processed products by ultra-high performance liquid chromatography with fluorescence detection. Journal of AOAC International, 102: 1666-1672.
Dhanshetty, M.P. and Malli, G.V. 2021. Isolation, characterisation of AF producing fungi from contaminated peanut and rice, their fate on AF production. YMER, 20(11): 1-11. Ellis, M. 1971. Dematiaceous Hyphomycetes. Commonwealth Mycological Institute. Kew, Surrey, UK.
European Commission. 2006. Commission Regulation (EC) No 401/2006 of 23 February 2006: Laying Down the Methods of Sampling and Analysis for the Official Control of Levels of Mycotoxins in Foodstuff. [https://eur-lex.europa.eu/legal-content/EN/TXT/PDF/?uri1⁄4CELEX: 32006R0401 &from1⁄4en] (accessed on 5 Oct., 2022).
Food Safety and Standards (Contaminants, Toxins and Residues) Regulations, 2011 (compendium) [https://www.fssai.gov.in/upload/uploadfiles/files/Compendium_Contaminants_Regulations_28 _01_2022.pdf] (accessed on 5 October 2022).
Gomez, A.A., Terán Baptista, Z.P., Mandova, T., Barouti, A., Kritsanida, M., Grougnet, R., Vattuone, M.A. and Sampietro, D.A. 2020. Antifungal and antimycotoxigenic metabolites from native plants of northwest Argentina: Isolation, identification and potential for control of Aspergillus species. Natural Product Research, 34(22): 3299-3302.
González-Salgado A., Gonzalez-Jaen T. and Vazquez C. 2008. Highly sensitive PCR-based detection method specific for Aspergillus flavus in wheat flour. Food Additive and Contaminates, 25: 758- 764.
Hassan, H.F., Kordahi, R., Dimassi, H., El Khoury, A., Daou, R., Alwan, N., Merhi, S., Haddad, J. and Karam, L. 2022. Aflatoxin B1 in rice: Effects of storage duration, grain type and size, production site, and season. Journal of Food Protection, 85(6): 938-944.
IARC. 1993. Some naturally occurring substances: Food items and constituents, heterocyclicaromatic amines and mycotoxins. pp. 489-521. In: IARC Monographs on the Evaluation of Carcinogenic Risks to Humans. IARC, Geneva, Switzerland.
Madden, T. 2003. The BLAST sequence analysis tool. The NCBI Handbook. NCBI, USA. Marchler-Bauer, A., Derbyshire, M.K., Gonzales, N.R., Lu, S., Chitsaz, F., Geer, L.Y., Geer, R.C., He, J., Gwadz, M., Hurwitz, D.I. and Lanczycki, C.J. 2015. CDD: NCBI's conserved domain database. Nucleic Acids Research, 43(D1): D222-D226. [https://academic.oup.com/nar/article/ 43/D1/D222/2439461].
Moss, M.O. 1998. Recent studies of mycotoxins. Journal of Applied Microbiology Symposium Supplement, 84: 62-76.
Mutegi, C.K., Wagacha, J.M., Christie, M.E., Kimani, J. and Karanja, L. 2013. Effect of storage conditions on quality and aflatoxin contamination of peanuts (Arachis hypogaea L.). International Journal of AgriScience, 3(10): 746-758.
Naumenko, N.V., Potoroko, I.Y., Malinin, A.V., Tsaturov, A.V. and Nazarova, O.D. 2019. Grain aflatoxins and ways to minimize accumulation risks. Bulletin of the South Ural University. Food and Biotechnology Series, 7: 70-80.
Ok, H.E., Chung, S.H., Lee, N. and Chun, H.S. 2015. Simple high-performance liquid chromatography method for the simultaneous analysis of aflatoxins, ochratoxin A, and zearalenone in dried and ground red pepper. Journal of Food Protection, 78(6): 1226-1231.
Oulkar, D., Goon, A., Dhanshetty, M., Khan, Z., Satav, S. and Banerjee, K. 2018. High-sensitivity direct analysis of AFs in peanuts and cereal matrices by ultra-performance liquid chromatography with fluorescence detection involving a large volume flow cell. Journal of Environmental Science and Health, Part B, 53(4): 255-260.
Ozbey, F. and Kabak, B. 2012. Natural co-occurrence of aflatoxins and ochratoxin A in spices. Food Control, 28(2): 354-361.
Raghuwanshi, S., Misra, S. and Saxena, R.K. 2014. Treatment of wheat straw using tannase and white-rot fungus to improve feed utilization by ruminants. Journal of Animal Science and Biotechnology, 5(1): 1-8.
Roncero, M.I.G., Hera, C., Ruiz-Rubio, M., Maceira, F.I.G., Madrid, M.P., Caracuel, Z., Calero, F., Delgado-Jarana, J., Roldán-Rodrıguez, R., Martınez-Rocha, A.L. and Velasco, C. 2003. Fusarium as a model for studying virulence. Physiological and Molecular Plant Pathology, 62(2): 87-98.
SANTE 2016. Guidance document on identification of mycotoxin in food and feed SANTE/12089/2016. [https://ec.europa.eu/food/sites/food/files/safety/docs/cs_contaminants_ sampling_guid-doc-ident-mycotoxins.pdf] (accessed on 5 Oct., 2022).
Sieber, P., Schorderet, M., Ryser, U., Buchala, A., Kolattukudy, P., Métraux, J.P. and Nawrath, C. 2000. Transgenic Arabidopsis plants expressing a fungal cutinase show alterations in the structure and properties of the cuticle and postgenital organ fusions. The Plant Cell, 12(5): 721- 737.
Sutton, B.C. 1980. The Coelomycetes. CMI, Kew, Surrey, England.
Manisha Dhanshetty and Gajanan Vishu Mali
Tamura, K., Stecher, G., Peterson, D., Filipski, A. and Kumar, S. 2013. MEGA6: Molecular evolutionary genetics analysis version 6.0. Molecular Biology and Evolution, 30(12): 2725- 2729.
Thathana, M.G., Murage, H., Abia, A.L.K. and Pillay, M. 2017. Morphological characterization and determination of aflatoxin-production potentials of Aspergillus flavus isolated from maize and soil in Kenya. Agriculture, 7(10): 80-81.
Uka, V., Moore, G.G., Arroyo-Manzanares, N., Nebija, D., De Saeger, S. and Diana Di Mavungu, J. 2019. Secondary metabolite dereplication and phylogenetic analysis identify various emerging mycotoxins and reveal the high intra-species diversity in Aspergillus flavus. Frontiers in Microbiology, 10: 667. [https://www.frontiersin.org/articles/10.3389/fmicb.2019.00667/full].
Zhang, Y., Li, M., Liu, Y., Guan, E. and Bian, K. 2020. Reduction of aflatoxin B1 in corn by water assisted microwaves treatment and its effects on corn quality. Toxins, 12(9): 605-608.
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