Extraction Of Sulfated Polysaccharide From Marine Macro Alga Gracilaria Edulis And Its Biological Applications
DOI:
https://doi.org/10.48165/Keywords:
Antibacterial activity, anticancer, antioxidant, Gracilaria edulis, sulfated polysaccharideAbstract
In present study, a red macroalga Gracilaria edulis, collected from Rameshwaram Tamil Nadu (India) was used to extract sulfated polysaccharide by using cold acidic extraction method. The yield of sulfated polysaccharide was 2.35 g from 10 g dry powdered G. edulis. The extracted sulfated polysaccharide was analysed for antibacterial, anticancer, antioxidant and anticoagulant activities. The antibacterial activity of crude sulfated polysaccharide was examined on three pathogenic bacteria viz., Escherichia coli, Klebsiella pneumoniae and Staphylococcus aureus using well diffusion method. The zone of inhibition was 20 ± 0.05 mm in case of S. aureus, followed by E. coli and K. pneumoniae with zone of inhibitions of 15 ± 0.04 and 12 ±0.02 mm, respectively, at 50 μg mL-1sulfated polysaccharide. The anticancer activity was performed using HeLa cancer cell line and inhibitory concentration (IC50) was 57.1 μg mL−1 as per MTT assay method. The antioxidant activity was analysed by performing 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid (ABTS) assay and the inhibitory concentration (IC50) value of 174.62 μg mL-1 was observed. The anticoagulant assay was tested and partial thromboplastin time (PTT) increased by 4.5 sec at 40 μg mL-1 of sulfated polysaccharide using activated partial thromboplastin time (APTT) assay method.
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Arunkumar, K., Raja, R., Kumar, V.S., Joseph, A., Shilpa, T. and Carvalho, I.S. 2021. Antioxidant and cytotoxic activities of sulfated polysaccharides from five different edible seaweeds. Journal of Food Measurement and Characterization, 15: 567-576.
Balakumaran, M.D., Ramachandran, R. and Kalaichelvan, P.T. 2015. Exploitation of endophytic fungus, Guignardia mangiferae for extracellular synthesis of silver nanoparticles and their in vitro biological activities. Microbiological Research, 178: 9-17.
Balakumaran, M.D., Ramachandran, R., Balashanmugam, P., Mukeshkumar, D.J. and Kalaichelvan, P.T. 2016. Mycosynthesis of silver and gold nanoparticles: optimization, characterization and antimicrobial activity against human pathogens. Microbiological Research, 182: 8-20.
Bhuyar, P., Sundararaju, S., Rahim, M.H.A., Unpaprom, Y., Maniam, G.P. and Govindan, N. 2021. Antioxidative study of polysaccharides extracted from red (Kappaphycus alvarezii), green (Kappaphycus striatus) and brown (Padina gymnospora) marine macroalgae/seaweed. SN Applied Sciences, 3: 1-9.
Chaluvanahalli S.T., Krishnamoorthy, E., Remadevi Gopakumar, L., Ebeneezar, S., Devi, H.M., Korpulliyil Kasim, A. and Mathew, S. 2021. Valorisation of brown seaweed (Sargassum wightii) waste as a feed ingredient in rohu (Labeo rohita): Effects on growth and metabolism. Aquaculture Nutrition, 27(6): 2662-2672.
Chen, X., Song, L., Wang, H., Liu, S., Yu, H., Wang, X. and Li, P. 2019. Partial characterization, the immune modulation and anticancer activities of sulfated polysaccharides from filamentous microalgae Tribonema sp. Molecules, 24(2): 322. [doi.org/10.3390/molecules24020322].
Dhargalkar, V.K. and Kavlekar, D.P. 2004. Seaweeds - A Field Manual. National Institute of Oceanography, Dona Paula, Goa, India
Fidelis, M., de Moura, C., Kabbas Junior, T., Pap, N., Mattila, P., Mäkinen, S. and Granato, D. 2019. Fruit seeds as sources of bioactive compounds: Sustainable production of high value-added ingredients from by-products within circular economy. Molecules, 24(21): 3854. [doi.org/10.3390/molecules24213854].
Gomez, K.A. and Gomez, A.A. 1984. Statistical Procedures for Agricultural Research (2nd edn.). John Wiley & Sons, New York, USA.
Hemalatha, V., Silambarasan, T. and Dhandapani, R. 2021. Extraction, purification, characterization and in vitro Antibacterial properties of sulfated polysaccharide extracted using Sargassum sp. Research Journal of Pharmacy and Technology, 14(9): 4991-4998.
Jasem, M., Merai, A.A. and Nizam, A.A. 2022. Characterization, and in vitro antibacterial activity of sulfated polysaccharides from freshwater algae Cladophora crispata. Access Microbiology, 5 (37): 1-15.
Karthick, V., Akhila, C., Kumar, V.G., Subashini, D., Dhas, T.S., Govindaraju, K. and Vasanth, K. 2019. In vitro anticancer activity of Sargassum sp. polysaccharides against MCF-7 cell lines. Indian Journal of Geo Marine Sciences, 48(8): 1267-1273
V. Hemalatha et al.
Li, X., Xiong, F., Liu, Y., Liu, F., Hao, Z. and Chen, H. 2018. Total fractionation and characterization of the water-soluble polysaccharides isolated from Enteromorpha intestinalis. International Journal of Biological Macromolecules, 111: 319-325.
Meinita, M.D.N., Akromah, N., Andriyani, N., Setijanto, S., Harwanto, D. and Liu, T. 2021. Molecular identification of Gracilaria species (Gracilariales, Rhodophyta) obtained from the South Coast of Java Island, Indonesia. Biodiversitas Journal of Biological Diversity, 22(7): 3046- 3056.
Mendhulkar, V.D., Shetye, L.A. and Khot, O. 2020. Modulation of the anti-cancer activity of sulfated polysaccharides, synthesized in Spirulina platensis, due to varying degree of sulfation induced by nutrient and physical stress. Journal of Biologically Active Products from Nature, 10(4): 275- 284.
Mousavian, Z., Safavi, M., Azizmohseni, F., Hadizadeh, M. and Mirdamadi, S. 2022. Characterization, antioxidant and anticoagulant properties of exopolysaccharide from marine microalgae. AMB Express, 12(1): 27. [doi.org/10.1186/s13568-022-01365-2].
Muthukumar, J., Chidambaram, R. and Sukumaran, S. 2021. Sulfated polysaccharides and its commercial applications in food industries - A review. Journal of Food Science and Technology, 58: 2453-2466.
Padmanaban, D., Samuel, A., Sahayanathan, G.J., Raja, K. and Chinnasamy, A. 2022. Anticancer effect of marine bivalves derived polysaccharides against human cancer cells. Biocatalysis and Agricultural Biotechnology, 39: 102240. [doi.org/10.1016/j.bcab.2021.102240].
Palani, K., Balasubramanian, B., Malaisamy, A., Maluventhen, V., Arumugam, V.A., Al-Dhabi, N. A. and Arumugam, M. 2022. Sulfated polysaccharides derived from Hypnea valentiae and their potential of antioxidant, antimicrobial, and anticoagulant activities with in silico docking. Evidence-Based Complementary and Alternative Medicine, 37(6): 1-15.
Safavi, S.V., Kenari, A.A., Tabarsa, M. and Esmaeili, M. 2019. Effect of sulfated polysaccharides extracted from marine macroalgae (Ulva intestinalis and Gracilariopsis persica) on growth performance, fatty acid profile, and immune response of rainbow trout (Oncorhynchus mykiss). Journal of Applied Phycology, 31: 4021-4035.
Saidani, K., Ziani, N., Touati, N., Merzouk, H. and Bedjou, F. 2021. Anticoagulant activity of sulfated polysaccharides and polyphenols extracted from marine algae. Current Bioactive Compounds, 17(3): 246-255.
Souza, R.B., Frota, A.F., Silva, J., Alves, C., Neugebauer, A.Z., Pinteus, S. and Benevides, N.M.B. 2018. In vitro activities of kappa-carrageenan isolated from red marine alga Hypnea musciformis: Antimicrobial, anticancer and neuroprotective potential. International Journal of Biological Macromolecules, 112: 1248-1256.
Susanti, F., Adharini, R.I., Rahmi, K.A., Kartika Sari, D.W. and Kandasamy, G. 2022. Identification of Gracilaria spp. in Gunungkidul Regency, Yogyakarta Indonesia based on DNA barcoding target cytochrome oxidase subunit 1. Indonesian Journal of Marine Sciences, 27(3): 189-198.
Wang, N., Dai, L., Chen, Z., Li, T., Wu, J., Wu, H. and Xiang, W. 2021. Extraction optimization, physicochemical characterization, and antioxidant activity of polysaccharides from Rhodosorus sp. SCSIO-45730. Journal of Applied Phycology, 34: 285-299.
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