Mutagenic Impact Of Ethyl Methane Sulphonate On  Morphological And Cytological Effects In Berseem  (Trifolium Alexandrinum L.)

Girjesh Kumar; Kaushal Tripathi; Moni Mishra

doi:10.48165/

Authors

Girjesh Kumar Naithani Plant Genetics Laboratory, Department of Botany, University of Allahabad, Prayagraj - 211 002 (India)
Kaushal Tripathi Naithani Plant Genetics Laboratory, Department of Botany, University of Allahabad, Prayagraj - 211 002 (India)
Moni Mishra Naithani Plant Genetics Laboratory, Department of Botany, University of Allahabad, Prayagraj - 211 002 (India)

DOI:

https://doi.org/10.48165/

Keywords:

Berseem, chlorophyll mutants, cytological abnormalities, ethyl methane sulphonate, mutagenesis, mutation breeding

Abstract

Induced mutagenesis has been effectively demonstrated as one of the best possible methodology for the genetic improvement of crop species. Egyptian clover (Trifolium alexandrinum L.), an important green fodder plant species of the family Fabaceae with immense agricultural value, was selected for the present study to improve its genetics by using the strategy of induced mutagenesis. Dry and healthy seeds of Egyptian clover cv. ‘Wardan’ were exposed to different doses (0.1, 0.3 and 0.5%) of Ethyl methane sulphonate (EMS) for variable time (3 and 5 h). The germination and survival percentages were recorded in M1 generation and 0.3% EMS at 5 h was determined as LD50 dose; whereas frequency of chlorophyll mutants and viable mutants along with cytological observations were screened in M2 generation. A broad spectrum of chlorophyll and viable mutants with different frequencies were observed. Cytological abnormalities increased along with rising EMS concentrations and mutagen exposure time. The findings are encouraging and show that significant genetic variability had been induced by EMS. Thus, tcomprehensive selection of the desirable mutants may bring about advancement of improved and high yielding mutants of T. alexandrinum in upcoming generation.

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References

Agarwal, R. and Ansari, M.Y.K. 2001. The effect of aniline on root tip cells of Vicia faba L. Journal of Cytology and Genetics, 2: 129-134.

Ahloowalia, B.S., Maluszynski, M. and Nichterlein, K. 2004. Global impact of mutation-derived varieties. Euphytica, 135: 187-204.

Ambarkar, A.S. 1997. Mutagenesis Studies in Chickpea. M.Sc. thesis, Botany Department, College of Agriculture, Pune, India.

Anitha, V., Ando, A. and Montalvan, R. 2005. Estimation of diosgenin in seeds of induced autoploid Trigonellafoenum-graecum. Fitoterapia, 58: 51-52.

Arisha, M.H., Shah, S.N., Gong, Z.H., Jing, H., Li, C. and Zhang, H.X. 2015. Ethyl methane sulfonate induced mutations in M2 generation and physiological variations in M1 generation of peppers (Capsicum annuum L.). Frontiers in Plant Science, 6: 399-410.

Baptista-Giacomelli, F.R., Pagliarini, M.S. and de Almeida, J.L. 2000. Meiotic behavior in several Brazilian oat cultivars (Avena sativa L.). Cytologia, 65: 371-378.

Bhat, T.A., Khan, A.H. and Parveen, S. 2006. Effect of gamma rays on certain cytomorphological parameters in two varieties of Vicia faba (L.). Advances in Plant Sciences, 19: 227-235. Dhakshanamoorthy, D., Selvaraj, R. and Chidambaram, A. 2010. Physical and chemical mutagenesis in Jatropha curcas L. to induce variability in seed germination, growth and yield traits. Romanian Journal of Plant Biology, 55: 113-125.

Gnanamurthy, S. and Dhanavel, D. 2014. Effect of EMS on induced morphological mutants and chromosomal variation in cowpea (Vigna unguiculata (L.) Walp). International Letters in Natural Sciences, 17: 33-43.

Jabee, F. and Ansari, M.Y.K. 2005. Mutagenic effectiveness and efficiency of hydrazine sulphate (HS) in inducing cytomorphological mutation in Cicer arietinum L. var. K. 850. Journal of Cytology and Genetics, 6:161-166.

Jabee, F., Ansari, M.Y.K. and Shahab, D. 2008. Studies on the effect of maleic hydrazide on root tip cells and pollen fertility in Trigonellafoenum-graecum L. Turkish Journal of Botany, 32: 337- 344.

Kavina, J., Ranjith, V.S. and Sathya, B. 2020. Effect of EMS on chlorophyll mutagen in fenugreek (Trigonellafoenum-graecum L.). Journal of Medicinal Plants, 8: 1-5.

Kharkwal, M.C. 1998. Induced mutations in chickpea (Cicer arietinum L.) II. Frequency and spectrum of chlorophyll mutations. Indian Journal of Genetics and Plant Breeding, 58: 465-474. Kolar, F., Pawar, N. and Dixit, G. 2011 Induced chlorophyll mutations in Delphinium malabaricum (Huth) Munz. Journal of Applied Horticulture, 13: 18-24.

Kumar, G. and Gupta, P. 2009. Induced karyomorphological variations in three phenodeviants of Capsicum annuum L. Turkish Journal of Biology, 33: 123-128.

Kumar, G. and Rai, P.K. 2007. EMS induced karyomorphological variations in maize (Zea mays L.) inbreds. Turkish Journal of Biology, 31: 187-195.

Marks, G.E. 1954. Anaceto-carmine glycerol jelly for use in pollen-fertility counts. Stain Technology, 29: 277-277.

Mehta, R.K. and Swaminathan, M.S. 1975. Pusa giant berseem. Indian Farming, 15: 4-6. Nirmala, A. and Rao, P.N. 1996. Genetics of chromosome numerical mosaism in higher plants. Nucleus, 39: 151-175.

Girjesh Kumar et al.

Pathak, S., Malaviya, D.R., Kumar Roy, A., Dwivedi, K. and Kaushal, P. 2015. Multifoliate leaf formation in induced tetraploids of Trifoliumalexandrinum L. Cytologia, 80: 59-66. Rajoriya, C.M., Rayees, A., Rawat, R.S. and Jat, B.L. 2016. Studies on induction of mutation in fenugreek (Trigonellafonum-graecum). International Journal for Research in Applied Science and Engineering Technology, 4: 333-373.

Rao, P.N., Ranganadham, P. and Nirmala, A. 1990. Behaviour of a sticky-desynaptic mutant in pearl millet. Genetica, 81: 221-227.

Reddy, P. and Munirajappa, M. 2012. Gamma ray induced meiotic abnormalities in S13 mulberry. International Journal of Science and Nature, 3: 170-172.

Roychowdhury, R. and Tah, J. 2011. Chemical mutagenic action on seed germination and related agro metrical traits in M1 Dianthus generation. Current Botany, 2: 19-23.

Shah, T.M., Mirza, J.I., Haq, M.A. and Atta, B.M. 2006. Induced genetic variability in chickpea (Cicer arietinum L.). I. Frequency and spectrum of chlorophyll mutations. Pakistan Journal of Botany, 38: 1217-1226.

Sikora, P., Chawade, A., Larsson, M., Olsson, J. and Olsson, O. 2011. Mutagenesis as a tool in plant genetics, functional genomics, and breeding. International Journal of Plant Genomics, 2011: 1- 13. [doi:10.1155/2011/314829].

Singh, V.P., Man, S. and Lal, J.P. 2000. Gamma ray and EMS induced genetic variability for quantitative traits in urdbean (Vigna mungo L. Hepper). Indian Journal of Genetics & Plant Breeding, 60: 89-96.

Solanki, I.S. and Sharma, B. 2001. Frequency and spectrum of chlorophyll mutations in macrosperma lentil (Lens culinaris Medik.). Indian Journal of Genetics and Plant Breeding, 61: 283-286. Srivastava, A. and Kapoor, K. 2008. Seed yield is not impaired by chromosome stickiness in sodium azide treated Trigonellafoenum-graecum. Cytologia, 73: 115-121.

Verma, A.K., Dhanasekar, P., Choudhary, S., Meena, R.D. and Lal, G. 2018. Estimation of induced variability in M2 generation of fennel (Foeniculum vulgare Mill,). Journal of Pharmacognosy and Phytochemistry, 7: 430-436.

Yaqoob, M. 2001. Induced mutation studies in some mung bean (Vigna radiata L.) Wilczek cultivars. Online Journal of Biological Sciences, 1: 805-808.