In Vitro Evaluation Of Antioxidant And Anticancer Properties Of Methanolic Extracts Of Ulva Paschima Against A549 And H1299 Lung Cancer Cell Lines

Authors

  • Digvijay Singh Yadav Department ofCentral University of Punjab, Ghudda, Bathinda – 151 401, Punjab (India) Botany,
  • Alka Rani Department of Botany, Central University of Punjab, Ghudda, Bathinda – 151 401, Punjab (India)
  • Amit Kumar Laboratory of Natural Products, Department of Pharmaceutical Sciences and Natural Products, Central University of Punjab, Ghudda, Bathinda – 151 401, Punjab (India)
  • Vikas Jaitak Laboratory of Natural Products, Department of Pharmaceutical Sciences and Natural Products, Central University of Punjab, Ghudda, Bathinda – 151 401, Punjab (India)
  • Felix Bast Department of Botany,Central University of Punjab, Ghudda, Bathinda – 151 401, Punjab (India)

Keywords:

Crude extract, GC-MS, mitochondrial membrane potential, seaweed, Ulva paschima

Abstract

The present study focused on the antiproliferative effect ofmethanolicextractsofUlvapaschim(MEUP) on lung cancer cell lines A549 and H1299. The  superoxide dismutase, catalase and glutathione reductase activities in MEUP  wereassayed.Thintracellular reactive oxygen species, antioxidant potential,  mitochondrial membrane potential and cell proliferation ability were also determineusing standard methods while the extracts were analysed by GCMS. The study showed that MEUP had selective antiproliferative effect on cancer cell lines with IC50 0.187 ± 0.120 µg µL-1for A549 and 0.130 ± 0.031 µg µL-1for H1299  cells as determined by 3-(4,5-dimethylthiazol-2-cyl)-2,5-diphenyltetrazolium  bromide (MTT) assay. MEUP induced oxidative stress in dose-dependent  manner as measured by dihydro-ethidium (DHE) and 2′,7′-dichloro-dihydro fluorescein diacetate (H2DCFDA) assays. Due to the induced oxidative stress by  MEUP treatment, a rise in intracellular levels of antioxidant enzymes  (glutathione reductase, superoxide dismutase, and catalase) was observed. A  dose-dependent depolarization of mitochondrial membrane potential by 38%  for A549 and 41% for H1299 cell lines was observed. The 2,2-diphenyl-1- picrylhydrazyl (DPPH) radical scavenging activity was 61.69% as compared to  the ascorbic acid with EC50 of 176.7 ± 0.09 µg mL-1. GC-MS analysis of MEUP showed the presence of 1-dodecanol, 5-eicosene, neophytadiene, stearic acid,  lauric acid, hexadecanoic acid, margaric acid, linoleic acid, L-α-terpineol, oleic  acid, 9-octadecenamide, myristic acid and phytol known for their anticancerous  properties. The phytochemical profile was evaluated for potential for serving as  novel anticancer drug candidates. 

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Published

2023-02-02

Issue

Vol. 25 No. 1 (2023): Applied Biological Research (Jan) 2023

Section

Review Articles

How to Cite

In Vitro Evaluation Of Antioxidant And Anticancer Properties Of Methanolic Extracts Of Ulva Paschima Against A549 And H1299 Lung Cancer Cell Lines . (2023). Applied Biological Research, 25(1), 19–28. Retrieved from https://acspublisher.com/journals/index.php/abr/article/view/11621